soil microflora
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Author(s):  
Sezer Şahin ◽  
Seda Bice Ataklı

Organic matter has a positive effect on the uptake of nutrients in the soil. The increase in mycorrhizal activities in the soil causes an increase in the activities in the rhizosphere region. Vermicompost is among the most useful organic fertilizers that can be added to the soil in recent years. Soil microflora is capable of realizing the return of mineral substances. Akkız cowpea variety was used in the study: The experiment was carried out as a viol study with 3 replications according to the randomized blocks trial design. In the study, seedling development was achieved by mixing vermicompost (0, 15, 30 and 45%) with and without mycorrhiza in a soil containing 16% lime into the viols in order to develop seedlings. Sufficient minerals have been applied to the plants for their growth. Seedlings were harvested when they were 40 days old, and seedling measurement parameters and macronutrients in plant leaves were checked. As the amount of vermicompost increased, it caused an increase in the above-ground fresh and dry weights, root fresh and root dry weights of the seedlings. As the organic fertilizer mixture increased, the nitrogen, phosphorus, potassium and magnesium concentrations of the plant leaves increased. The addition of mycorrhiza to the rearing medium has led to different results in the investigated characteristics. Addition of increasing organic fertilizer ratios in the study, Organic fertilizer and bacteria application of cowpea cultivars were effective in increasing the development of plants in calcareous soils.


2022 ◽  
Vol 07 ◽  
Author(s):  
Punit Kumar ◽  
Sujata Malik ◽  
Kashyap Kumar Dubey

Background: The present world population is about 7.9 billion and it is increasing continuously. Thus, there is an urgent requirement to enhance the agricultural output sustainably. Agricultural approaches such as the use of advanced agriculture methods, high productivity varieties, and enhanced application of fertilizers and pesticides have significantly increased food grain production but in an unsustainable way. Chemical-based conventional fertilizers and pesticides have been found associated with environmental pollution and other unwanted effects on the ecosystem, soil quality, and soil microflora, etc. Nanomaterials may be used to replace conventional fertilizers and pesticides in agriculture. Objective: The aim of this review is to provide information about the harmful effects of chemical fertilizers and pesticides, and the use of nanomaterials in agriculture. Including this, the health risks of nanomaterials are discussed. Method: This review article includes a survey of literature from different online sources (for example, Web of Science, PubMed, and Google Scholar, etc.). Results: The improvement in agricultural output using chemical fertilizers and pesticides is considered unsustainable as it is increasing the cost of production, affecting the soil quality, disturbing nutrient availability in crops, and causing environmental pollution. Nanotechnology is a potent innovative practice and nanomaterials may be used in agriculture as nanofertilizers, nanopesticides, and nanosensors. Although these approaches have the potential to enhance agricultural productivity in a sustainable way, nanomaterials are also assumed to exhibit potential health risks to humans. Reports have indicated that nanomaterials have been found associated with many systematic diseases such as cardiovascular diseases, neurotoxicity, and toxicity to the reproductive system, etc. Conclution: It is well accepted that chemical fertilizers and pesticides in agriculture cause environmental toxicity and affect ecosystem activity. Nanomaterials have the potential to enhance agricultural output, but these are also associated with health risks. Thus, detailed scientific studies must be conducted about the potential health risk of nanomaterials before their commercial applications in agriculture.


Plant Disease ◽  
2022 ◽  
Author(s):  
Maria Lodovica Gullino ◽  
Angelo Garibaldi ◽  
Abraham Gamliel ◽  
Jaacov Katan

This Feature Article tracks 100 years of soil disinfestation: from the goal of eradicating soilborne pathogens and pests to much milder approaches, aimed at establishing a healthier soil, by favoring or enhancing the beneficial soil microflora and introducing biological control agents. The restrictions on use of many chemical fumigants is favoring the adoption of nonchemical strategies, from soilless cultivation to the use of physical or biological control measures, with more focus on maintaining soil microbial diversity, thus enhancing soil and plant health. Such approached are described and discussed, with special focus on their integrated use.


Agriculture ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1214
Author(s):  
Huijie Chen ◽  
Jiamiao Zhao ◽  
Jing Jiang ◽  
Zhiguo Zhao ◽  
Zhiyong Guan ◽  
...  

The production of chrysanthemums is severely hampered by Fusarium wilt, which is exacerbated by monoculture. In this study, the role of inorganic plant nutrition fertilizer (IPN), organic fertilizer (OF) and bio-organic fertilizer (BOF) in avoiding monoculture-related production constraints was evaluated. We conducted a series of greenhouse experiments and studied the growth of chrysanthemum and changes in rhizosphere soil microflora and function. BOF application reduced the incidence of Fusarium wilt by 82.8% and increased the chrysanthemum shoot height and flower ray floret number by 31.4% and 26.1%, respectively. High-throughput Illumina HiSeq2500 sequencing results indicated that BOF and OF treatments increased the values of α-diversity indices of bacteria and fungi. In addition, significant alterations in microbe community structures were found in response to IPN, OF or BOF application. Among the major genera detected after BOF treatments, the levels of Fusarium and Glycomyces decreased while Cladosporium, arbuscular mycorrhizal and endophyte groups increased. In particular, the abundance of Mariniflexile had a positive relationship (R = 0.693, p < 0.05) with the incidence of Fusarium wilt, while Cladosporium showed a significant negative relationship (R = −0.586, p < 0.05). Interestingly, an analysis of microbiomes based on 16S rRNA sequences revealed that the functions of signal transduction, bacterial secretion system, oxidative phosphorylation and the metabolism of carbohydrate, nitrogen and amino acids all increased in both BOF and OF treatments. The results suggested that BOF could be effective for chrysanthemum monoculture soil restoration, potentially by altering the microbial community structures and functions, which affect the physiological and morphological attributes of chrysanthemum in monoculture.


2021 ◽  
Vol 2114 (1) ◽  
pp. 012068
Author(s):  
Wijdan Ahmed Ali ◽  
Rahaf Haineed Hussein ◽  
Wafaa Tali Radef

Abstract Microorganisms are a varied collection of organisms that make up around 60% of the earth’s biomass. The ratio of fungal to plant species is estimated to be around 6 to 1. In order to extrapolate worldwide estimates of 1.5 million fungal species, this ratio is utilized. Because of the activity of soil organisms, soils are very complex systems with numerous components performing many roles. Soil microflora is essential for assessing soil conditions and encouraging plant development. Microorganisms are helpful in enhancing soil fertility because they participate in a variety of biochemical transformation and mineralization processes in soils. Organic matter in the soil that impacts the physical, chemical, and biological characteristics of the soil and serves as a complementary medium for biological processes and life support in the soil environment. Microbes have a very significant role in biodiversity. Organic matter in the soil that influences its physical, chemical, and biological properties and acts as a complimentary medium for biological activities and life support in the soil environment. Microbes play an important part in biodiversity. The study demonstrated the importance of pH and soil texture on the variety of soil fungus species. Three clones were grown on PDA at 28 C° (7 clays) as Aspergillus sp., Penicillium sp., and Fusarium sp.


2021 ◽  
Vol 22 (21) ◽  
pp. 12006
Author(s):  
Spartak S. Khutsishvili ◽  
Alla I. Perfileva ◽  
Olga A. Nozhkina ◽  
Tatjana V. Ganenko ◽  
Konstantin V. Krutovsky

New promising manganese-containing nanobiocomposites (NCs) based on natural polysaccharides, arabinogalactan (AG), arabinogalactan sulfate (AGS), and κ-carrageenan (κ-CG) were studied to develop novel multi-purpose trophic low-dose organomineral fertilizers. The general toxicological effects of manganese (Mn) on the vegetation of potatoes (Solanum tuberosum L.) was evaluated in this study. The essential physicochemical properties of this trace element in plant tissues, such as its elemental analysis and its spectroscopic parameters in electron paramagnetic resonance (EPR), were determined. Potato plants grown in an NC-containing medium demonstrated better biometric parameters than in the control medium, and no Mn accumulated in plant tissues. In addition, the synthesized NCs demonstrated a pronounced antibacterial effect against the phytopathogenic bacterium Clavibacter sepedonicus (Cms) and were proved to be safe for natural soil microflora.


2021 ◽  
Vol 25 (11) ◽  
pp. 34-37
Author(s):  
Anis Naushi ◽  
Ajay Kumar Arya

This investigation was aimed toward assessing the impact of heavy metals on soil microbial cycles. The impacts of lead (Pb) and cadmium (Cd) at various concentrations were researched over a time of about two months. Chloride salts of Pb and Cd were added independently and in blend to soil samples at room temperature (27ºC) in various polythene packs. Samples were taken from the sacks at about fourteen days span and estimations were taken of the microbial biomass carbon (MBC). The outcomes showed that there was a significant reduction in the microbial biomass carbon for all treated soils from the second week to the 6th week. However, on 8th week, increase in microbial biomass carbon was observed. At the 6th week, 2000mgkg-1Pb and 40mgkg-1Cd gave the main reduction (P < 0.05) in microbial biomass carbon of 98%. A critical decrease in biomass carbon in metal contaminated soil demonstrated that this parameter is a decent marker of toxicity of metals on soil microflora.


2021 ◽  
Vol 7 (1) ◽  
pp. 43-48
Author(s):  
Thi Thom Nguyen ◽  
Van Binh Nguyen ◽  
Bang Diep Tran ◽  
Dang Sang Hoang ◽  
Xuan An Tran ◽  
...  

Bacillus thuringiensis (Bt) produces different types of toxin that have potent and specific insecticidal activity. In recent years, Bt toxins have been used as the safe biological control agents to protect crops replacing for chemical insecticides. Bt-based biopesticides that have been commercialized as the alternative products to control pests and insects for sustainable agriculture, contain toxicity crystals and a significant number of spores that affects to the soil microflora. These uncontrollable changes may contaminate the cultivation soil, and eventually cause adverse effects to human and animal health. Therefore, the living cells and spores existing in the Bt-biopesticides should be controlled. This study evaluates the effects of gamma radiation on spore viability, germination and growth of the existing spores after spraying on the soil and the insecticidal effectiveness of a Bt-based biopesticide (VBT) against lepidoptera larvae. We attempted to identify the optimal dose that couldinactivate Bt spores but the toxicity of Bt still retain highly. The results revealed that the dose of 20 kGy is enough to control all living cells and spores in the product that consists of approximately 5.2 × 107 spores in the initial VBT. Though the growth of existing spores after spraying on the soil reduced by 85% or more by irradiation, their insecticidal activity against Heliothis armigera larvae reduced by 20-30% only as compared to that of the initial VBT. It suggested that gamma irradiation can be applied as useful way to control the living cells and spores existing in the commercial Bt-based bio-pesticides, and the radiation dose of 20 kGy is enough to kill all spores in VBT, but still kept its insecticidal effect for Heliothis armigera larvae


2021 ◽  
Author(s):  
Shilpa . ◽  
Parveen Sharma ◽  
Priyanka Bijalwan

As the population of India is continuously rising so we have to adopt some means of sustaining our agricultural growth and it can be done through conservation farming. The best way out is the adoption of the age old practice of mulching in our agricultural fields. Mulching is an agricultural and horticultural technique in which the use of organic materials and synthetic materials for the purpose of increasing soil productivity is involved. In rainfed area, judicious use of water is essential for improving crop productivity. Therefore, mulching has been advocated as an effective means for conserving soil moisture. This technique is very useful in protecting the roots of the plants from heat, cold or drought or to keep fruit free from diseases and insect pests. Mulching is an old age technique to conserve soil moisture, checks evaporation, check the weed growth, moderate soil temperature and provide congenial microclimatic conditions for plant growth and development. This technology is a boon for the horticulture crops not only for increasing growth, development, yield but also a method for soil and water conservation. This review paper deals with the various effects of mulching on plants, soil as well as on soil microflora. This technology has brought a great revolution in the agriculture especially for water conservation and proves to be a fastest growing plasticultural technique in the world.


2021 ◽  
Vol 16 (1) ◽  
pp. 186-194
Author(s):  
Domenico Prisa

Research goal: Based on the information found in the literature on biochar, its ability to improve the quality and yield of productions and to influence soil microflora. In this work, studies were carried out at different concentrations in pots to assess whether the addition of this material to the growing medium can influence the growth and control of biotic and abiotic stresses on Astrophytum myriostigma and Astrophytum capricorne. Materials and Methods: The experiments, started in June 2020, were conducted in the greenhouses of CREA-OF in Pescia (PT), Tuscany, Italy (43°54′N 10°41′E). The experimental groups were: i) group control, irrigated with water and substrate previously fertilized; ii) group with biochar 5%, irrigated with water and substrate previously fertilized; iii) group with biochar 10%, irrigated with water and substrate previously fertilized; iv) group with biochar 15%, irrigated with water and substrate previously fertilized; On June 30, 2021, plant height and circumference, number and length of thorns, vegetative weight, root weight, number of fruits, fruit weight, number of flowers, flower life, plants dead from cold stress, plants dead from Rhizoctonia solani. Results and Discussion: The test showed a significant increase in the agronomic parameters analyzed in biochar-treated plants on Astrophytum myriostigma and Astrophytum capricorne. The test also showed an improvement in plant quality in terms of increased number of thorns, fruits, flowers and increased resistance to cold stress and the pathogen Rhizoctonia solani. The trial also showed that an increase in substrate biochar content of 10%-15% improved plant growth performance compared to lower 5% contents. Conclusions: The experimentation carried out at CREA-OF in Pescia showed how the application of biochar to the cultivation substrate of Astrophytum myriostigma and Astrophytum capricorne can significantly improve the quality and the resistance to biotic and abiotic stresses of the plants. As in other experiments in the literature, the application of this soil conditioner also resulted in positive effects in the control of pathogenic fungi, particularly Rhizoctonia solani. This effect was probably mediated by the presence of microorganisms in the biochar that, in addition to having an effect on plant stimulation, may have a fundamental role in their biocontrol activity of substrate pathogens. Interesting results have also been the increase in the number of thorns, flowers and fruit duration and resistance to cold stress in plants grown with biochar.


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