Increased tolerance to methyl viologen by transgenic tobacco plants that over-express the cytosolic glutathione reductase gene fromBrassica campestris

2004 ◽  
Vol 47 (2) ◽  
pp. 111-116 ◽  
Author(s):  
Hyoshin Lee ◽  
Jinki Jo
2003 ◽  
Vol 160 (4) ◽  
pp. 347-353 ◽  
Author(s):  
Suk-Yoon Kwon ◽  
Sun-Mee Choi ◽  
Young-Ock Ahn ◽  
Haeng-Soon Lee ◽  
Hae-Bok Lee ◽  
...  

2000 ◽  
Vol 156 (4) ◽  
pp. 504-509 ◽  
Author(s):  
Byung-Wook Yun ◽  
Gyung-Hye Huh ◽  
Haeng-Soon Lee ◽  
Suk-Yoon Kwon ◽  
Jin-Ki Jo ◽  
...  

2003 ◽  
Vol 160 (11) ◽  
pp. 1305-1311 ◽  
Author(s):  
T.a.o. Yu ◽  
Yang Sheng Li ◽  
Xue Feng Chen ◽  
Jing Hu ◽  
X.u.n. Chang ◽  
...  

2005 ◽  
Vol 14 (3) ◽  
pp. 251-259 ◽  
Author(s):  
Helga Schinkel ◽  
Andreas Schiermeyer ◽  
Raphael Soeur ◽  
Rainer Fischer ◽  
Stefan Schillberg

Author(s):  
Ai-Hua Wang ◽  
Lan Yang ◽  
Xin-Zhuan Yao ◽  
Xiao-Peng Wen

AbstractPhosphoethanolamine N-methyltransferase (PEAMTase) catalyzes the methylation of phosphoethanolamine to produce phosphocholine and plays an important role in the abiotic stress response. Although the PEAMT genes has been isolated from many species other than pitaya, its role in the drought stress response has not yet been fully elucidated. In the present study, we isolated a 1485 bp cDNA fragment of HpPEAMT from pitaya (Hylocereus polyrhizus). Phylogenetic analysis showed that, during its evolution, HpPEAMT has shown a high degree of amino acid sequence similarity with the orthologous genes in Chenopodiaceae species. To further investigate the function of HpPEAMT, we generated transgenic tobacco plants overexpressing HpPEAMT, and the transgenic plants accumulated significantly more glycine betaine (GB) than did the wild type (WT). Drought tolerance trials indicated that, compared with those of the wild-type (WT) plants, the roots of the transgenic plants showed higher drought tolerance ability and exhibited improved drought tolerance. Further analysis revealed that overexpression of HpPEAM in Nicotiana tabacum resulted in upregulation of transcript levels of GB biosynthesis-related genes (NiBADH, NiCMO and NiSDC) in the leaves. Furthermore, compared with the wild-type plants, the transgenic tobacco plants displayed a significantly lower malondialdehyde (MDA) accumulation and higher activities of the superoxide dismutase (SOD) and peroxidase (POD) antioxidant enzymes under drought stress. Taken together, our results suggested that HpPEAMT enhanced the drought tolerance of transgenic tobacco.


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