<p><em>TaPRP</em>, a proline rich protein (PRP) cDNA, was cloned by RT-PCR from winter wheat. Nucleotide sequence analysis showed <em>TaPRP</em> is composed of 1137 bp (378 amino acid residues with a Mr of 42.19 kD).<em> TaPRP</em> shows 92.6%, 89.3%, 73.0%, and 73.3% sequence homologies with <em>PRP</em> genes from wheat, sorghum, rice, and maize, respectively. The deduced protein includes 170 prolines, presenting a normal <em>PRP</em> primary structure. Expression vector pBI<em>-TaPRP</em> was constructed, in which <em>TaPRP</em> was driven by CaMV35S promoter and stopped by NospolyA. Tobaccos were transformed by<em> Agrobacterium</em> containing the constructed vectors. Three transgenic lines were confirmed by PCR detection and Southern blot. Under the same low temperature stress conditions, transgenic plants had lower conductivity rate compared with the non-transgenic plants, suggesting that cold tolerance in transgenic tobacco plants was improved. However, the different transgenic plants showed significant differences in cold resistant, and there also existed significant interactions between plant and treatment temperature. <em>TaPRP </em>might have an important role in wheat in cold adaptation process.</p>
Genetic Enhancement of Cold Tolerance by Expression of a Gene for Chloroplast [omega]-3 Fatty Acid Desaturase in Transgenic Tobacco