Transgenic Tobacco Plants Over-expressing Arabidopsis Transcriptional Factor CBF1 Show Morphological and Biochemical Characteristics Associated with Cold Tolerance

2006 ◽  
Vol 5 (6) ◽  
pp. 932-939 ◽  
Author(s):  
Shanjun Wei . ◽  
Zhenyuan Sun . ◽  
Lei Han . ◽  
Longjiang Yu .
Keyword(s):  
Cold Tolerance ◽  
Transgenic Tobacco ◽  
Transcriptional Factor ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Biochemical Characteristics

Cloning of an antifreeze protein gene from carrot and its influence on cold tolerance in transgenic tobacco plants

2002 ◽  
Vol 21 (4) ◽  
pp. 296-301 ◽  
Author(s):  
Y. Fan ◽  
B. Liu ◽  
H. Wang ◽  
S. Wang ◽  
J. Wang
Keyword(s):  
Cold Tolerance ◽  
Transgenic Tobacco ◽  
Protein Gene ◽  
Antifreeze Protein ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants

Transgenic tobacco plants over expressing cold regulated protein CbCOR15b from Capsella bursa-pastoris exhibit enhanced cold tolerance

2012 ◽  
Vol 169 (14) ◽  
pp. 1408-1416 ◽  
Author(s):  
Lihua Wu ◽  
Mingqi Zhou ◽  
Chen Shen ◽  
Jing Liang ◽  
Juan Lin
Keyword(s):  
Cold Tolerance ◽  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants

Production of an active recombinant thrombomodulin derivative in transgenic tobacco plants and suspension cells

2005 ◽  
Vol 14 (3) ◽  
pp. 251-259 ◽  
Author(s):  
Helga Schinkel ◽  
Andreas Schiermeyer ◽  
Raphael Soeur ◽  
Rainer Fischer ◽  
Stefan Schillberg
Keyword(s):  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Suspension Cells ◽  
Tobacco Plants ◽  
Recombinant Thrombomodulin

scholarly journals Overexpression of the pitaya phosphoethanolamine N-methyltransferase gene (HpPEAMT) enhanced simulated drought stress in tobacco

2021 ◽  
Author(s):  
Ai-Hua Wang ◽  
Lan Yang ◽  
Xin-Zhuan Yao ◽  
Xiao-Peng Wen
Keyword(s):  
Drought Stress ◽  
Stress Response ◽  
Transgenic Plants ◽  
Drought Tolerance ◽  
Transgenic Tobacco ◽  
Sequence Similarity ◽  
Amino Acid Sequence Similarity ◽  
Transgenic Tobacco Plants ◽  
Wild Type ◽  
Tobacco Plants

AbstractPhosphoethanolamine N-methyltransferase (PEAMTase) catalyzes the methylation of phosphoethanolamine to produce phosphocholine and plays an important role in the abiotic stress response. Although the PEAMT genes has been isolated from many species other than pitaya, its role in the drought stress response has not yet been fully elucidated. In the present study, we isolated a 1485 bp cDNA fragment of HpPEAMT from pitaya (Hylocereus polyrhizus). Phylogenetic analysis showed that, during its evolution, HpPEAMT has shown a high degree of amino acid sequence similarity with the orthologous genes in Chenopodiaceae species. To further investigate the function of HpPEAMT, we generated transgenic tobacco plants overexpressing HpPEAMT, and the transgenic plants accumulated significantly more glycine betaine (GB) than did the wild type (WT). Drought tolerance trials indicated that, compared with those of the wild-type (WT) plants, the roots of the transgenic plants showed higher drought tolerance ability and exhibited improved drought tolerance. Further analysis revealed that overexpression of HpPEAM in Nicotiana tabacum resulted in upregulation of transcript levels of GB biosynthesis-related genes (NiBADH, NiCMO and NiSDC) in the leaves. Furthermore, compared with the wild-type plants, the transgenic tobacco plants displayed a significantly lower malondialdehyde (MDA) accumulation and higher activities of the superoxide dismutase (SOD) and peroxidase (POD) antioxidant enzymes under drought stress. Taken together, our results suggested that HpPEAMT enhanced the drought tolerance of transgenic tobacco.

Overexpression of an Apocynum venetum flavonols synthetase gene confers salinity stress tolerance to transgenic tobacco plants

2021 ◽  
Author(s):  
Meng Wang ◽  
Tingting Ren ◽  
Ruihuan Huang ◽  
Yiqiang Li ◽  
Chengsheng Zhang ◽  
...  
Keyword(s):  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Salinity Stress ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Apocynum Venetum ◽  
Salinity Stress Tolerance

scholarly journals Expression of a tuber-specific storage protein in transgenic tobacco plants: demonstration of an esterase activity

1987 ◽  
Vol 6 (5) ◽  
pp. 1155-1159 ◽  
Author(s):  
S. Rosahl ◽  
J. Schell ◽  
L. Willmitzer
Keyword(s):  
Transgenic Tobacco ◽  
Storage Protein ◽  
Esterase Activity ◽  
Transgenic Tobacco Plants ◽  
Specific Storage ◽  
Tobacco Plants

scholarly journals Transgenic Tobacco Plants Expressing Pea Chloroplast Nmdh cDNA in Sense and Antisense Orientation (Effects on NADP-Malate Dehydrogenase Level, Stability of Transformants, and Plant Growth)

1997 ◽  
Vol 115 (2) ◽  
pp. 705-715 ◽  
Author(s):  
M. Faske ◽  
J. E. Backhausen ◽  
M. Sendker ◽  
M. Singer-Bayrle ◽  
R. Scheibe ◽  
...  
Keyword(s):  
Plant Growth ◽  
Transgenic Tobacco ◽  
Malate Dehydrogenase ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Orientation Effects ◽  
Antisense Orientation
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