Expression of hup Genes in Azorhizobium caulinodans and Their Coregulation With RuBP Carboxylase

1998 ◽  
Vol 7 (2) ◽  
pp. 115-118
Author(s):  
Sandhya Sanghi ◽  
M. L. Lodha
Keyword(s):  
Azorhizobium Caulinodans ◽  
Rubp Carboxylase ◽  
Hup Genes

scholarly journals FixJ family regulator AcfR of Azorhizobium caulinodans is involved in symbiosis with the host plant

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Wei Liu ◽  
Xue Bai ◽  
Yan Li ◽  
Haikun Zhang ◽  
Xiaoke Hu
Keyword(s):  
Signal Transduction ◽  
Host Plant ◽  
Type Strain ◽  
Wild Type Strain ◽  
Response Regulator ◽  
Azorhizobium Caulinodans ◽  
Wild Type ◽  
Response Regulators ◽  
Free Living ◽  
Two Component

Abstract Background A wide variety of bacterial adaptative responses to environmental conditions are mediated by signal transduction pathways. Two-component signal transduction systems are one of the predominant means used by bacteria to sense the signals of the host plant and adjust their interaction behaviour. A total of seven open reading frames have been identified as putative two-component response regulators in the gram-negative nitrogen-fixing bacteria Azorhizobium caulinodans ORS571. However, the biological functions of these response regulators in the symbiotic interactions between A. caulinodans ORS571 and the host plant Sesbania rostrata have not been elucidated to date. Results In this study, we identified and investigated a two-component response regulator, AcfR, with a phosphorylatable N-terminal REC (receiver) domain and a C-terminal HTH (helix-turn-helix) LuxR DNA-binding domain in A. caulinodans ORS571. Phylogenetic analysis showed that AcfR possessed close evolutionary relationships with NarL/FixJ family regulators. In addition, six histidine kinases containing HATPase_c and HisKA domains were predicted to interact with AcfR. Furthermore, the biological function of AcfR in free-living and symbiotic conditions was elucidated by comparing the wild-type strain and the ΔacfR mutant strain. In the free-living state, the cell motility behaviour and exopolysaccharide production of the ΔacfR mutant were significantly reduced compared to those of the wild-type strain. In the symbiotic state, the ΔacfR mutant showed a competitive nodule defect on the stems and roots of the host plant, suggesting that AcfR can provide A. caulinodans with an effective competitive ability for symbiotic nodulation. Conclusions Our results showed that AcfR, as a response regulator, regulates numerous phenotypes of A. caulinodans under the free-living conditions and in symbiosis with the host plant. The results of this study help to elucidate the involvement of a REC + HTH_LuxR two-component response regulator in the Rhizobium-host plant interaction.

Restoration of the wild-type locus in an RuBP carboxylase/oxygenase mutant of Synecbocystis PCC 6803 via targeted gene recombination

1992 ◽  
Vol 235 (2-3) ◽  
pp. 247-252 ◽  
Author(s):  
Doron Amichay ◽  
Meir Sheffer ◽  
Michael Gurevitz
Keyword(s):  
Gene Recombination ◽  
Wild Type ◽  
Rubp Carboxylase ◽  
Type Locus ◽  
Pcc 6803

Phage specificity and lipopolysaccarides of stem- and root-nodulating bacteria (Azorhizobium caulinodans, Sinorhizobium spp., and Rhizobium spp.) of Sesbania spp.

2007 ◽  
Vol 189 (4) ◽  
pp. 411-418 ◽  
Author(s):  
Radhey Shyam Sharma ◽  
Vandana Mishra ◽  
Asif Mohmmed ◽  
Cherukuri Raghavendra Babu
Keyword(s):  
Azorhizobium Caulinodans ◽  
Rhizobium Spp ◽  
Sesbania Spp

Inhibition of Assimilatory Nitrate Uptake by Ammonium Ions in Azorhizobium caulinodans IRBG 46

1996 ◽  
Vol 5 (2) ◽  
pp. 119-121 ◽  
Author(s):  
K. Siva Raju ◽  
N. D. Sharma ◽  
M. L. Lodha
Keyword(s):  
Nitrate Uptake ◽  
Azorhizobium Caulinodans ◽  
Ammonium Ions

scholarly journals COLONIZATION of Rubus glaucus BENTH BY AZORHIZOBIUM CAULINODANS ORS571 USIG A FLAVONOIDE NARINGENINA

2016 ◽  
Vol 14 (2) ◽  
pp. 15
Author(s):  
Giovani Orlando Cancino Escalante ◽  
S E Cancino ◽  
Enrique Quevedo Garcia
Keyword(s):  
Bacterial Colonization ◽  
Lateral Roots ◽  
Plant Growth Promoting Bacteria ◽  
Azorhizobium Caulinodans ◽  
Intensity Parameters ◽  
Plant Growth Promoting ◽  
Measuring Frequency ◽  
Northwest Region ◽  
Azorhizobium Caulinodans Ors571

Root systems of two Andean blackberry materials (thorn and thornless) of Rubus glaucus Benth cultured in vitro in the presence of five treatments (four flavonoids and one control) were inoculated with Azorhizobium caulinodans ORS571 (pXLGD4)  (a strain carrying the lacZ reporter gene which facilitated the detection of bacterial colonization). Evaluation of colonization effectiveness for each treatment was done by means of application of experimental design measuring frequency and intensity parameters. Statistical analysis showed differences at comparing flavonoids vs. control and the overall higher effectiveness of the flavonoid naringenin. Observation of colonization was made by light and electron microscope confirming internal colonization of Andean blackberry roots by A. caulinodans. This is the first work demonstrating root colonization of R.glaucus by azorhizobia and therefore settling the basis for future investigations and scientific applications related to interaction with plant growth-promoting bacteria under the effect of flavonoids, along with possible implications of common benefit for non-legume crops in the northwest region of Colombia.  Key Words: Azorhizobium caulinodans ORS571, Andean blackberry, flavonoids, LacZ, lateral roots, naringenin. 

scholarly journals Cloning of an Azorhizobium caulinodans endoglucanase gene and analysis of its role in symbiosis.

1995 ◽  
Vol 61 (9) ◽  
pp. 3304-3310 ◽  
Author(s):  
D Geelen ◽  
M van Montagu ◽  
M Holsters
Keyword(s):  
Azorhizobium Caulinodans ◽  
Endoglucanase Gene

scholarly journals Abscisic acid as a factor in regulation of photosynthetic carbon metabolism of pea seedlings

2014 ◽  
Vol 51 (2) ◽  
pp. 229-240 ◽  
Author(s):  
Maria Faltynowicz ◽  
Waldemar Lechowicz ◽  
Jerzy Poskuta
Keyword(s):  
Abscisic Acid ◽  
Carbon Metabolism ◽  
Rubp Carboxylase ◽  
Pep Carboxylase ◽  
Pea Seedlings ◽  
Photosynthetic Carbon ◽  
Photosynthetic Carbon Metabolism ◽  
Sugar Phosphates ◽  
Reduced Activity ◽  
Endogenous Aba

The influence of abscisic acid (ABA) on carbon metabolism and the activity of ribulosebisphosphate (RuBP) and phosphoenolpyruvate (PEP) carboxylases in 8-day-old pea seedlings was investigated. It was endeavoured to correlate the changes observed in metabolic processes with the endogenous ABA level. In plants treated with ABA incorporation of labeled carbon into sucrose, glucose, fructose and sugar phosphates was depressed, while <sup>14</sup>C incorporation into starch, ribulose and malic acid was enhanced. The activity of RuBP carboxylase was considerably lowered, whereas that of PEP carboxylase was slightly increased. It is considered that inhibition of photosynthesis due to the action of ABA is caused to a great extent by the obstruction of the C-3 pathway and reduced activity of RuBP carboxylase, whereas (β-carboxylation was not blocked.

Genetic engineering in agriculture: hydrogen uptake (hup) genes

1980 ◽  
Vol 5 (6) ◽  
pp. 167-170 ◽  
Author(s):  
S.T. Lim ◽  
K. Andersen ◽  
R. Tait ◽  
R.C. Valentine
Keyword(s):  
Genetic Engineering ◽  
Hydrogen Uptake ◽  
Hup Genes

Nucleotide sequence of the fixABC region of Azorhizobium caulinodans ORS571: similarity of the fixB product with eukaryotic flavoproteins, characterization of fixX, and identification of nifW

1991 ◽  
Vol 225 (3) ◽  
pp. 514-520 ◽  
Author(s):  
Fabrizio Arigoni ◽  
P. Alexandre Kaminski ◽  
Hauke Hennecke ◽  
Claudine Elmerich
Keyword(s):  
Nucleotide Sequence ◽  
Azorhizobium Caulinodans ◽  
Azorhizobium Caulinodans Ors571
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