Optimized fed-batch fermentation of L-β-hydroxy isobutyric acid by Yarrowia lipolytica

1999 ◽  
Vol 20 (3) ◽  
pp. 189-193
Author(s):  
H. S. Kim ◽  
J. Y. Ju ◽  
J. H. Suh ◽  
C. S. Shin
Keyword(s):  
Yarrowia Lipolytica ◽  
Batch Fermentation ◽  
Isobutyric Acid ◽  
Fed Batch ◽  
Fed Batch Fermentation

Improvement of Yarrowia lipolytica lipase production by fed-batch fermentation

2008 ◽  
Vol 49 (2) ◽  
pp. 212-215 ◽  
Author(s):  
Patrick Fickers ◽  
Jacqueline Destain ◽  
Philippe Thonart
Keyword(s):  
Yarrowia Lipolytica ◽  
Batch Fermentation ◽  
Lipase Production ◽  
Fed Batch ◽  
Fed Batch Fermentation

Repeated fed-batch fermentation using biosensor online control for citric acid production by Yarrowia lipolytica

2011 ◽  
Vol 153 (3-4) ◽  
pp. 133-137 ◽  
Author(s):  
Lucie Moeller ◽  
Mario Grünberg ◽  
Andreas Zehnsdorf ◽  
Andreas Aurich ◽  
Thomas Bley ◽  
...  
Keyword(s):  
Citric Acid ◽  
Yarrowia Lipolytica ◽  
Acid Production ◽  
Batch Fermentation ◽  
Online Control ◽  
Citric Acid Production ◽  
Fed Batch ◽  
Fed Batch Fermentation

scholarly journals Efficient Conversion of Cane Molasses Towards High-Purity Isomaltulose and Cellular Lipid Using an Engineered Yarrowia lipolytica Strain in Fed-Batch Fermentation

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1228 ◽  
Author(s):  
Zhi-Peng Wang ◽  
Qin-Qing Wang ◽  
Song Liu ◽  
Xiao-Fang Liu ◽  
Xin-Jun Yu ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
High Purity ◽  
Batch Fermentation ◽  
Low Cost ◽  
Lipid Level ◽  
Corn Steep Liquor ◽  
Biodiesel Production ◽  
Fed Batch ◽  
Cane Molasses ◽  
Fed Batch Fermentation

: Cane molasses is one of the main by-products of sugar refineries, which is rich in sucrose. In this work, low-cost cane molasses was introduced as an alternative substrate for isomaltulose production. Using the engineered Yarrowia lipolytica, the isomaltulose production reached the highest (102.6 g L−1) at flask level with pretreated cane molasses of 350 g L−1 and corn steep liquor of 1.0 g L−1. During fed-batch fermentation, the maximal isomaltulose concentration (161.2 g L−1) was achieved with 0.96 g g−1 yield within 80 h. Simultaneously, monosaccharides were completely depleted, harvesting the high isomaltulose purity (97.4%) and high lipid level (12.2 g L−1). Additionally, the lipids comprised of 94.29% C16 and C18 fatty acids, were proved suitable for biodiesel production. Therefore, the bioprocess employed using cane molasses in this study was low-cost and eco-friendly for high-purity isomaltulose production, coupling with valuable lipids.

scholarly journals Dissolved-oxygen feedback control fermentation for enhancing β-carotene in engineered Yarrowia lipolytica

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Peng Jun Lv ◽  
Shan Qiang ◽  
Liang Liu ◽  
Ching Yuan Hu ◽  
Yong Hong Meng
Keyword(s):  
Dissolved Oxygen ◽  
Yarrowia Lipolytica ◽  
Batch Fermentation ◽  
Utilization Rate ◽  
Crabtree Effect ◽  
Fed Batch ◽  
Promising Strategy ◽  
Fed Batch Fermentation ◽  
Β Carotene ◽  
Gene Expression Levels

Abstract The DO-stat fed-batch fermentation was carried out to explore the volumetric productivity of β-carotene in engineered Yarrowia lipolytica C11 strain. Using DO-stat fed-batch fermentation, we achieved 94 g/L biomass and 2.01 g/L β-carotene. Both biomass and β-carotene were about 1.28-fold higher than that in fed-batch fermentation. The ATP, NADP+/NADPH, and gene expression levels of tHMG, GGS1, carRA, and carB were promoted as compared to that in fed-batch fermentation. As for as the kinetic parameters in DO-stat fed-batch fermentation, μm′, Yx/s′, and Yp/s′ was 0.527, 0.353, and 0.158, respectively. The μm′ was elevated 4.66-fold than that in fed-batch fermentation. These data illustrate that more dissolved oxygen increased the biomass. The Yx/s′ and Yp/s′ were increased 1.15 and 22.57-fold, which suggest that the DO-stat fed-batch fermentation reduced the Crabtree effect and improved the utilization rate of glucose. Therefore, DO-stat fed-batch fermentation is a promising strategy in the industrialized production of β-carotene.

Glycerol Synthesis Attenuation ofCandida glycerinogenesin Fed-batch Fermentation

2012 ◽  
Vol 18 (5) ◽  
pp. 791
Author(s):  
Xiaoyun DING ◽  
Bin ZHUGE ◽  
Huiying FANG ◽  
Hong ZONG ◽  
Xiaoxiao LIU ◽  
...  
Keyword(s):  
Batch Fermentation ◽  
Fed Batch ◽  
Fed Batch Fermentation ◽  
Glycerol Synthesis
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