rol-Gene expression in transgenic aspen (Populus tremula) plants results in accelerated growth and improved stem production index

Trees ◽  
1999 ◽  
Vol 14 (2) ◽  
pp. 49-54 ◽  
Author(s):  
T. Tzfira ◽  
Alexander Vainstein ◽  
Arie Altman
Keyword(s):  
Gene Expression ◽  
Populus Tremula ◽  
Production Index ◽  
Transgenic Aspen ◽  
Accelerated Growth ◽  
Rol Gene ◽  
Stem Production

scholarly journals Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions

2020 ◽  
Author(s):  
Elena O. Vidyagina ◽  
Natalia M. Subbotina ◽  
Vladimir A. Belyi ◽  
Vadim G. Lebedev ◽  
Konstantin V. Krutovsky ◽  
...  
Keyword(s):  
Cell Wall ◽  
Decomposition Rate ◽  
Transgenic Line ◽  
Carbohydrate Composition ◽  
Penicillium Canescens ◽  
Natural Conditions ◽  
Biochemical Traits ◽  
Transgenic Aspen ◽  
Transgenic Lines ◽  
Accelerated Growth

Abstract Background: Recombinant carbohydrases genes are used to produce transgenic woody plants with improved phenotypic traits. However, cultivation of such plants in open field is challenging due to by a number of problems. Therefore, additional research is needed to alleviate them. Results: Results of successful cultivation of the transgenic aspens ( Populus tremula ) carrying the recombinant xyloglucanase gene ( sp-Xeg ) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time. Change of carbohydrate composition of wood was observed in transgenic aspens carrying the sp-Xeg gene. The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt. The accelerated growth was observed also in the transgenic line Xeg-1-1c. Thicker cell-wall and longer xylem fiber were also observed in both these transgenic lines. Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines. The decomposition rate was approximately twice as lower for the transgenic line Xeg-2-3b in comparison with the control untransformed line Pt. Conclusion: A direct dependence of the phenotypic and biochemical traits on the expression of the recombinant gene sp-Xeg was demonstrated. The higher was the level of the sp-Xeg gene expression, the more markedly were changes in the phenotypic and biochemical traits. All lines showed phenotypic changes in the leave traits. Our results showed that the plants carrying the recombinant sp-Xeg gene do not demonstrate a decrease in growth parameters in semi-natural conditions. In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness and a decrease in the rate of decomposition of wood were observed.

scholarly journals Transgenic aspens (Populus tremula) with the xyloglucanase gene from Penicillium canescens keep faster growth under semi-natural conditions

2020 ◽  
Author(s):  
Elena O. Vidyagina ◽  
Natalia M. Subbotina ◽  
Vladimir A. Belyi ◽  
Vadim G. Lebedev ◽  
Konstantin V. Krutovsky ◽  
...  
Keyword(s):  
Cell Wall ◽  
Decomposition Rate ◽  
Transgenic Line ◽  
Populus Tremula ◽  
Phenotypic Traits ◽  
Carbohydrate Composition ◽  
Penicillium Canescens ◽  
Natural Conditions ◽  
Transgenic Lines ◽  
Accelerated Growth

Abstract Background Recombinant carbohydrases genes are used to produce transgenic woody plants with improved phenotypic traits. However, cultivation of such plants in open field is challenged by a number of problems. Therefore, additional research is needed to alleviate them. Results Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time. Change of carbohydrate composition of wood was observed in transgenic aspens carrying the sp-Xeg gene. The transformed transgenic line PtXVXeg1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt. The accelerated growth was observed also in the transgenic line PtXIVXeg1c. Thicker cell-wall and longer xylem fiber were also observed in both these transgenic lines. Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines. The decomposition rate was approximately twice as lower for the transgenic line PtXVXeg3b in comparison with the control untransformedline Pt. Conclusion Our results showed that the plants carrying the recombinant sp-Xeg gene do not demonstrate a decrease in growth parameters in semi-natural conditions. However, in some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness and a decrease in the rate of decomposition of wood are observed.

Phenotypic manifestation of gene expression encoding xyloglucanase from Penicillium canescens in transgenic aspen plants

2012 ◽  
Vol 59 (5) ◽  
pp. 618-625 ◽  
Author(s):  
K. A. Shestibratov ◽  
A. S. Podresov ◽  
M. A. Salmova ◽  
Yu. A. Kovalitskaya ◽  
E. O. Vidyagina ◽  
...  
Keyword(s):  
Gene Expression ◽  
Penicillium Canescens ◽  
Transgenic Aspen ◽  
Phenotypic Manifestation

scholarly journals Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Elena O. Vidyagina ◽  
Natalia M. Subbotina ◽  
Vladimir A. Belyi ◽  
Vadim G. Lebedev ◽  
Konstantin V. Krutovsky ◽  
...  
Keyword(s):  
Cell Wall ◽  
Decomposition Rate ◽  
Transgenic Line ◽  
Carbohydrate Composition ◽  
Penicillium Canescens ◽  
Natural Conditions ◽  
Biochemical Traits ◽  
Transgenic Aspen ◽  
Transgenic Lines ◽  
Accelerated Growth

Abstract Background Recombinant carbohydrases genes are used to produce transgenic woody plants with improved phenotypic traits. However, cultivation of such plants in open field is challenging due to a number of problems. Therefore, additional research is needed to alleviate them. Results Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time. Change of carbohydrate composition of wood was observed in transgenic aspens carrying the sp-Xeg gene. The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt. The accelerated growth was observed also in the transgenic line Xeg-1-1c. Thicker cell-wall and longer xylem fiber were also observed in both these transgenic lines. Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines. The decomposition rate was approximately twice as lower for the transgenic line Xeg-2-3b in comparison with the control untransformed line Pt. Conclusion A direct dependence of the phenotypic and biochemical traits on the expression of the recombinant gene sp-Xeg was demonstrated. The higher was the level of the sp-Xeg gene expression, the more pronounced were changes in the phenotypic and biochemical traits. All lines showed phenotypic changes in the leave traits. Our results showed that the plants carrying the recombinant sp-Xeg gene do not demonstrate a decrease in growth parameters in semi-natural conditions. In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.

scholarly journals Transgenic aspens (Populus tremula) with the xyloglucanase gene from Penicillium canescens keep faster growth under semi-natural conditions

2019 ◽  
Author(s):  
Elena O. Vidyagina ◽  
Natalia M. Subbotina ◽  
Vladimir A. Belyi ◽  
Vadim G. Lebedev ◽  
Konstantin V. Krutovsky ◽  
...  
Keyword(s):  
Cell Wall ◽  
Decomposition Rate ◽  
Transgenic Line ◽  
Populus Tremula ◽  
Phenotypic Traits ◽  
Carbohydrate Composition ◽  
Penicillium Canescens ◽  
Natural Conditions ◽  
Transgenic Lines ◽  
Accelerated Growth

Abstract Background Recombinant carbohydrases genes are used to produce transgenic woody plants with improved phenotypic traits. However, cultivation of such plants in open field is challenged by a number of problems. Therefore, additional research is needed to alleviate them. Results Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time. Change of carbohydrate composition of wood was observed in transgenic aspens carrying the sp-Xeg gene. The transformed transgenic line PtXVXeg1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt. The accelerated growth was observed also in the transgenic line PtXIVXeg1c. Thicker cell-wall and longer xylem fiber were also observed in both these transgenic lines. Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines. The decomposition rate was approximately twice as lower for the transgenic line PtXVXeg3b in comparison with the control untransformedline Pt. Conclusion Our results showed that the plants carrying the recombinant sp-Xeg gene do not demonstrate a decrease in growth parameters in semi-natural conditions. However, in some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness and a decrease in the rate of decomposition of wood are observed.

scholarly journals Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions

2020 ◽  
Author(s):  
Elena O. Vidyagina ◽  
Natalia M. Subbotina ◽  
Vladimir A. Belyi ◽  
Vadim G. Lebedev ◽  
Konstantin V. Krutovsky ◽  
...  
Keyword(s):  
Cell Wall ◽  
Decomposition Rate ◽  
Transgenic Line ◽  
Carbohydrate Composition ◽  
Penicillium Canescens ◽  
Natural Conditions ◽  
Biochemical Traits ◽  
Transgenic Aspen ◽  
Transgenic Lines ◽  
Accelerated Growth

Abstract Background: Recombinant carbohydrases genes are used to produce transgenic woody plants with improved phenotypic traits. However, cultivation of such plants in open field is challenging due to by a number of problems. Therefore, additional research is needed to alleviate them. Results: Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time. Change of carbohydrate composition of wood was observed in transgenic aspens carrying the sp-Xeg gene. The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt. The accelerated growth was observed also in the transgenic line Xeg-1-1c. Thicker cell-wall and longer xylem fiber were also observed in both these transgenic lines. Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines. The decomposition rate was approximately twice as lower for the transgenic line Xeg-2-3b in comparison with the control untransformed line Pt. Conclusion: A direct dependence of the phenotypic and biochemical traits on the expression of the recombinant gene sp-Xeg was demonstrated. The higher was the level of the sp-Xeg gene expression, the more markedly were changes in the phenotypic and biochemical traits. All lines showed phenotypic changes in the leave traits. Our results showed that the plants carrying the recombinant sp-Xeg gene do not demonstrate a decrease in growth parameters in semi-natural conditions. In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness and a decrease in the rate of decomposition of wood were observed.

Growth-phase-dependent gene expression profiling of poplar (Populus alba × Populus tremula var. glandulosa) suspension cells

2007 ◽  
Vol 131 (4) ◽  
pp. 599-613 ◽  
Author(s):  
Hyoshin Lee ◽  
Eun-Kyung Bae ◽  
So-Young Park ◽  
Andreas Sjödin ◽  
Jae-Soon Lee ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Growth Phase ◽  
Expression Profiling ◽  
Populus Tremula ◽  
Populus Alba ◽  
Suspension Cells

Bud Regeneration and Growth from Transgenic and Non-Transgenic Aspen (Populus tremula) Root Explants

1997 ◽  
pp. 217-219 ◽  
Author(s):  
B. Vinocur ◽  
T. Tzfira ◽  
A. Vainstein ◽  
A. Altman ◽  
M. Ziv
Keyword(s):  
Populus Tremula ◽  
Root Explants ◽  
Bud Regeneration ◽  
Transgenic Aspen
Sign in / Sign up

Export Citation Format

Share Document