Ring trial validation of single and multiplex real-time PCR methods for the detection and quantification of the allergenic food ingredients mustard, celery, soy, wheat and rye

2016 ◽  
Vol 12 (1) ◽  
pp. 55-72 ◽  
Author(s):  
Hans-Ulrich Waiblinger ◽  
Britta Boernsen ◽  
Carina Geppert ◽  
Anja Demmel ◽  
Verena Peterseil ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Allergenic Food ◽  
Food Ingredients ◽  
Ring Trial ◽  
Detection And Quantification

First ring-trial validation of real-time PCR methods for the quantification of allergenic food ingredients

2012 ◽  
Vol 235 (4) ◽  
pp. 619-630 ◽  
Author(s):  
Marion Siegel ◽  
Karin Schnur ◽  
Britta Boernsen ◽  
Klaus Pietsch ◽  
Hans-Ulrich Waiblinger
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Allergenic Food ◽  
Food Ingredients ◽  
Ring Trial

Food matrix standards for the quantification of allergenic food ingredients using real-time PCR

2013 ◽  
Vol 237 (2) ◽  
pp. 185-197 ◽  
Author(s):  
Marion Siegel ◽  
A. Mutschler ◽  
B. Boernsen ◽  
Klaus Pietsch ◽  
Hans-Ulrich Waiblinger
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Allergenic Food ◽  
Food Matrix ◽  
Food Ingredients

scholarly journals Collaborative ring trial of two real-time PCR assays for the detection of porcine- and chicken-derived material in meat products

PLoS ONE ◽  
2018 ◽  
Vol 13 (10) ◽  
pp. e0206609 ◽  
Author(s):  
Qiang Wang ◽  
Yicun Cai ◽  
Yuping He ◽  
Litao Yang ◽  
Liangwen Pan
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Meat Products ◽  
Ring Trial ◽  
Pcr Assays

scholarly journals Detection and quantification of Streptococcus pneumoniae from Iranian patients with pneumonia and individual carriers by real time PCR

2011 ◽  
Vol 10 (60) ◽  
pp. 12826-12832 ◽  
Author(s):  
Nomanpour Bizhan ◽  
Ghodousi Arash ◽  
Babaei Toraj ◽  
AliJavad Mousavi Seyd ◽  
Asadi Soroor ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Real Time ◽  
Real Time Pcr ◽  
Iranian Patients ◽  
Detection And Quantification

scholarly journals Detection of Peanut Allergen Traces with a Real Time PCR Assay - The Challenge to Protect Food-Allergic Consumers

2017 ◽  
Vol 7 (1) ◽  
pp. 32 ◽  
Author(s):  
Dimitra Houhoula ◽  
Stamatios Koussissis ◽  
Vladimiros Lougovois ◽  
John Tsaknis ◽  
Dimitra Kassavita ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Food Products ◽  
Molecular Techniques ◽  
Food Allergens ◽  
Pcr Assay ◽  
Peanut Allergen ◽  
Pcr Method ◽  
Detection And Quantification

The aim of the present study was the implementation of molecular techniques in the detection and quantification of allergic substances of peanut in various kinds of food products, e.g., breakfast cereals, chocolates and biscuits that are frequently related to allergies. In some cases, the presence of peanuts can be due to contamination during production and are not declared on the label. A total of 152 samples were collected from supermarkets and were analysed by a Real Time PCR method. The results indicated that 125 samples (83,3%) were found positive in peanut traces but the most important finding is that from the 84 samples that had no allergen declaration for peanuts, 48 (57,1%) of them were found positive. In conclusion, Real Time PCR can be a very important tool for the rapid detection and quantification of food allergens.

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