scholarly journals From two stages to one: acceleration of the induced membrane (Masquelet) technique using human acellular dermis for the treatment of non-infectious large bone defects

2020 ◽  
Vol 46 (2) ◽  
pp. 317-327 ◽  
Author(s):  
René Danilo Verboket ◽  
Maximilian Leiblein ◽  
Maren Janko ◽  
Alexander Schaible ◽  
Jan Claas Brune ◽  
...  
Keyword(s):  
Bone Defects ◽  
Induced Membrane ◽  
Acellular Dermis ◽  
Masquelet Technique ◽  
Two Stages ◽  
Large Bone ◽  
Large Bone Defects

Reconstruction of Large Bone Defects Using induced membrane Technique of Masquelet

2021 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
Mohammed soliman ◽  
Yousif Khira ◽  
.mohamed Elzoheiry ◽  
ahmad abdelwahab
Keyword(s):  
Bone Defects ◽  
Induced Membrane ◽  
Membrane Technique ◽  
Induced Membrane Technique ◽  
Large Bone ◽  
Large Bone Defects

Reconstruction of Large Bone Defects and Complex Non-Unions Using a Free Fibular Bone Graft and a Supplementary Allograft

2020 ◽  
Author(s):  
Selina Gaida ◽  
Uwe Schweigkofler ◽  
Wibke Moll ◽  
Michael Sauerbier ◽  
Reinhard Hoffmann
Keyword(s):  
Bone Graft ◽  
Case Series ◽  
Bone Defects ◽  
Bone Transplantation ◽  
Patient Comfort ◽  
Masquelet Technique ◽  
Specialized Center ◽  
Free Fibula ◽  
Large Bone ◽  
Large Bone Defects

AbstractLarge bone defects or complex pseudarthrosis represent an interdisciplinary challenge. Established surgical procedures include autogenous cancellous bone graft, the Masquelet technique or bone transfer via segment transport as well as free microvascular bone transplantation. However, the successful use of all these techniques requires a specialized center with great interdisciplinary expertise. In the following case series we describe the technique of free fibula transplantation and additional allograft. In both cases a good functional result with full mechanical strength of the affected extremity and satisfactory patient comfort has been achieved. In the second case, implant failure with the necessity of revision endoprosthetics occurred during the procedure.

scholarly journals Fabrication, vascularization and osteogenic properties of a novel synthetic biomimetic induced membrane for the treatment of large bone defects

Bone ◽  
2014 ◽  
Vol 64 ◽  
pp. 173-182 ◽  
Author(s):  
Liling Ren ◽  
Yunqing Kang ◽  
Christopher Browne ◽  
Julius Bishop ◽  
Yunzhi Yang
Keyword(s):  
Bone Defects ◽  
Induced Membrane ◽  
Large Bone ◽  
Large Bone Defects

scholarly journals Immunohistochemical Analysis Revealed the Expression of Bone Morphogenetic Proteins-4, 6, 7, and 9 in Human Induced Membrane Samples Treated With the Masquelet Technique

2021 ◽  
Author(s):  
Takahiro Niikura ◽  
Takahiro Oda ◽  
Naoe Jimbo ◽  
Masato Komatsu ◽  
Keisuke Oe ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Bone Morphogenetic Proteins ◽  
Immunohistochemical Analysis ◽  
Bone Defects ◽  
Positive Control ◽  
Cement Spacer ◽  
Induced Membrane ◽  
Masquelet Technique ◽  
Human Bone Tissue ◽  
Large Bone

Abstract BackgroundInduced membrane (IM) is the key component of Masquelet reconstruction surgery for the treatment of bone defects. It is formed around the cement spacer and is known to secrete growth factors and osteoinductive factors. However, information on the presence of osteoinductive factors in IM is not enough in the literature. The purpose of this study was to investigate the existence of bone morphogenetic proteins (BMPs) in the IM harvested from patients during the treatment of bone defects using the Masquelet technique.MethodsWe included six patients whose bone defects were treated using the Masquelet technique. The affected bone was the femur in three patients and the tibia in three patients. During the second-stage surgery, 1-cm2 pieces of IM were harvested. Histological sections of IM were immunostained with anti-BMP-4, 6, 7, and 9 antibodies. Human bone tissue served as the positive control.ResultsThe existence of BMP-4, 6, 7, and 9 was observed in all IM samples. Further, immunolocalization of BMP-4, 6, 7, and 9 was observed in blood vessels and fibroblasts of all IM samples. Immunolocalization of BMP-4, 6, 7, and 9 was also observed in bone tissue within the IM in one sample, in which osteogenesis inside the IM was observed.ConclusionsThis study revealed that osteoinductive factors BMP-4, 6, 7, and 9 were present in the IM harvested from patients. This helps explain how the Masquelet technique effectively contributes to the healing of large bone defects. It may thus be possible for surgeons to omit the addition of BMPs to bone grafts give the endogenous secretion of BMPs from the IM.Trial registrationNot applicable.

scholarly journals MEMBRANE INDUCED OSTEOGENESIS (MASQUELET TECHNIQUE) VERSUS BONE TRANSPORT IN MANAGEMENT OF LARGE BONE DEFECTS OF THE LOWER LIMB

2020 ◽  
Vol 71 (1) ◽  
pp. 161-170
Author(s):  
Salah Abou-Seif ◽  
Mootaz Thakeb ◽  
Ahmed Yousry ◽  
Mahmoud Mahran ◽  
Tamer Fayyad ◽  
...  
Keyword(s):  
Lower Limb ◽  
Bone Defects ◽  
Bone Transport ◽  
Masquelet Technique ◽  
Large Bone ◽  
Large Bone Defects

scholarly journals Immunohistochemical analysis revealed the expression of bone morphogenetic proteins-4, 6, 7, and 9 in human induced membrane samples treated with the Masquelet technique

2022 ◽  
Vol 17 (1) ◽  
Author(s):  
Takahiro Niikura ◽  
Takahiro Oda ◽  
Naoe Jimbo ◽  
Masato Komatsu ◽  
Keisuke Oe ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Bone Morphogenetic Proteins ◽  
Immunohistochemical Analysis ◽  
Bone Defects ◽  
Positive Control ◽  
Cement Spacer ◽  
Induced Membrane ◽  
Masquelet Technique ◽  
Human Bone Tissue ◽  
Large Bone

Abstract Background Induced membrane (IM) is the key component of Masquelet reconstruction surgery for the treatment of bone defects. IM is formed around the cement spacer and is known to secrete growth factors and osteoinductive factors. However, there is limited evidence available concerning the presence of osteoinductive factors in IM. This study aimed to investigate the existence of bone morphogenetic proteins (BMPs) in IM harvested from patients during the treatment of bone defects using the Masquelet technique. Methods This study involved six patients whose bone defects had been treated using the Masquelet technique. The affected sites were the femur (n = 3) and the tibia (n = 3). During the second-stage surgery, 1 cm2 pieces of IM were harvested. Histological sections of IM were immunostained with anti-BMP-4, 6, 7, and 9 antibodies. Human bone tissue served as the positive control. Results The presence of BMP-4, 6, 7, and 9 was observed in all IM samples. Further, immunolocalization of BMP-4, 6, 7, and 9 was observed in blood vessels and fibroblasts in all IM samples. Immunolocalization of BMP-4, 6, 7, and 9 was also observed in bone tissue within the IM in one sample, in which osteogenesis inside the IM was observed. Conclusions This study showed that osteoinductive factors BMP-4, 6, 7, and 9 were present in the IM harvested from patients, providing evidence indicating that the Masquelet technique effectively contributes to healing large bone defects. Therefore, it may be possible for surgeons to omit the addition of BMPs to bone grafts, given the endogenous secretion of BMPs from the IM.

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