A genetic linkage map of water yam (Dioscorea alata L.) based on AFLP markers and QTL analysis for anthracnose resistance

2002 ◽  
Vol 105 (5) ◽  
pp. 726-735 ◽  
Author(s):  
H. Mignouna ◽  
R. Mank ◽  
T. Ellis ◽  
N. van den Bosch ◽  
R. Asiedu ◽  
...  
PLoS ONE ◽  
2018 ◽  
Vol 13 (10) ◽  
pp. e0197717 ◽  
Author(s):  
Ranjana Bhattacharjee ◽  
Christian O. Nwadili ◽  
Christopher A. Saski ◽  
Agre Paterne ◽  
Brian E. Scheffler ◽  
...  

2018 ◽  
Author(s):  
Ranjana Bhattacharjee ◽  
Christian O Nwadili ◽  
Christopher A Saski ◽  
Agre Paterne ◽  
Brian E. Scheffler ◽  
...  

AbstractWater yam (Dioscorea alata L.) is one of the most important food yams with wide geographical distribution in tropics. One of the major constraints to water yam production is anthracnose disease caused by a fungus, Colletotrichum gloesporioides (Penz.). There are no economically feasible solutions as chemical sprays or cultural practices, such as crop rotation are seldom convenient for smallholder farmers for sustainable control of the disease. Breeding for development of durable genetic resistant varieties is known to offer lasting solution to control endemic disease threats to crop production. However, breeding for resistance to anthracnose has been slow considering the biological constraints related to the heterozygous and vegetative propagation of the crop. The development of saturated linkage maps with high marker density, such as SSRs, followed by identification of QTLs can accelerate the speed and precision of resistance breeding in water yam. A total of 380 EST-SSRs were used to generate a saturated linkage map. About 60.19% of SSRs showed Mendelian segregation pattern, however, it had no effect on the construction of linkage map. All 380 EST-SSRs were mapped into 20 linkage groups covering a total length of 2559.66 cM, which agrees with the diploid nature (2n = 2x = 20) of the parents used in the cross. Majority of the markers were mapped on linkage group 1 comprising of 97 EST-SSRs. This is the first genetic linkage map of water yam constructed using EST-SSRs. QTL localization was based on phenotypic data collected over a 3-year period of inoculating the mapping population with the most virulent strain of C. gloeosporoides from West Africa. Using the mean permutation value of LOD scores as threshold value for declaring a putative QTL on all linkage groups, one QTL was consistently observed on linkage group (LG) 14 in all the three years and average score data. This QTL was found at position interval of 71.12 – 84.76 cM explaining 68.94% of the total phenotypic variation in the average score data. The high marker density allowed identification of QTLs and association for anthracnose disease, which could be validated in other mapping populations and used in marker-assisted breeding in D. alata improvement programmes.


2005 ◽  
Vol 110 (4) ◽  
pp. 669-677 ◽  
Author(s):  
A. Hamwieh ◽  
S. M. Udupa ◽  
W. Choumane ◽  
A. Sarker ◽  
F. Dreyer ◽  
...  

2018 ◽  
pp. 25-30
Author(s):  
D. Torello Marinoni ◽  
N. Valentini ◽  
E. Portis ◽  
A. Acquadro ◽  
C. Beltramo ◽  
...  

2019 ◽  
Vol 99 (5) ◽  
pp. 599-610
Author(s):  
Junhuan Zhang ◽  
Haoyuan Sun ◽  
Li Yang ◽  
Fengchao Jiang ◽  
Meiling Zhang ◽  
...  

A high-density genetic map of apricot (Prunus armeniaca L.) was constructed using an F1 population constructed by crossing two main Chinese cultivars ‘Chuanzhihong’ and ‘Luotuohuang’, coupled with a recently developed reduced representation library (RRL) sequencing. The average sequencing depth was 38.97 in ‘Chuanzhihong’ (female parent), 33.05 in ‘Luotuohuang’ (male parent), and 8.91 in each progeny. Based on the sequencing data, 12 451 polymorphic markers were developed and used in the construction of the genetic linkage map. The final map of apricot comprised eight linkage groups, including 1991 markers, and covered 886.25 cM of the total map length. The average distance between adjacent markers was narrowed to 0.46 cM. Gaps larger than 5 cM only accounted for <0.33%. To our knowledge, this map is the densest genetic linkage map that is currently available for apricot research. It is a valuable linkage map for quantitative trait loci (QTLs) identification of important agronomic traits. Moreover, the high marker density and well-ordered markers that this linkage map provides will be useful for molecular breeding of apricot as well. In this study, we applied this map in the QTL analysis of an important agronomic trait, pistil abortion. Several QTLs were detected and mapped respectively to the middle regions of LG5 (51.005∼59.4 cM) and LG6 (72.884∼76.562 cM), with nine SLAF markers closely linked to pistil abortion. The high-density genetic map and QTLs detected in this study will facilitate marker-assisted breeding and apricot genomic study.


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