Molecular tagging and genetic mapping of the disease resistance gene RppQ to southern corn rust

2003 ◽  
Vol 108 (5) ◽  
pp. 945-950 ◽  
Author(s):  
C. X. Chen ◽  
Z. L. Wang ◽  
D. E. Yang ◽  
C. J. Ye ◽  
Y. B. Zhao ◽  
...  
Genome ◽  
1999 ◽  
Vol 42 (4) ◽  
pp. 735-743 ◽  
Author(s):  
A Joyeux ◽  
M G Fortin ◽  
R Mayerhofer ◽  
A G Good

Genetic mapping of plants traditionally involves the analysis of large segregating populations. However, not all individuals in a population contribute equal amounts of genetic information. It is thus possible to achieve rough mapping using a subset of the most informative individuals in the population. We have designed a minimal Brassica napus mapping population of 23 doubled-haploid plants and have tested this method using this population in the mapping of disease resistance gene homologues in B. napus. Several groups have identified such homologues in soybean and potato by amplifying sequences corresponding to conserved nucleotide-binding sites from known resistance genes. However, the sequence conservation in the leucine-rich repeat domain that is present in most of the disease resistance genes isolated has not been exploited via the polymerase chain reaction (PCR). We present the genetic mapping of Brassica napus DNA sequences amplified with primers corresponding to both the nucleotide-binding site and the leucine rich-repeat domain of the Arabidopsis thaliana RPS2 gene. We also describe a method for the quick mapping of resistance gene homologues using the polymerase chain reaction.Key words: Brassica napus, disease resistance genes, minimal mapping population, RFLP markers.


2012 ◽  
Vol 34 (1) ◽  
pp. 56
Author(s):  
Ling CHEN ◽  
Hao ZHANG ◽  
Xian-Qin QIU ◽  
Hui-Jun YAN ◽  
Qi-Gang WANG ◽  
...  

Genetics ◽  
2002 ◽  
Vol 162 (4) ◽  
pp. 1961-1977
Author(s):  
Michelle A Graham ◽  
Laura Fredrick Marek ◽  
Randy C Shoemaker

Abstract PCR amplification was previously used to identify a cluster of resistance gene analogues (RGAs) on soybean linkage group J. Resistance to powdery mildew (Rmd-c), Phytophthora stem and root rot (Rps2), and an ineffective nodulation gene (Rj2) map within this cluster. BAC fingerprinting and RGA-specific primers were used to develop a contig of BAC clones spanning this region in cultivar “Williams 82” [rps2, Rmd (adult onset), rj2]. Two cDNAs with homology to the TIR/NBD/LRR family of R-genes have also been mapped to opposite ends of a BAC in the contig Gm_Isb001_091F11 (BAC 91F11). Sequence analyses of BAC 91F11 identified 16 different resistance-like gene (RLG) sequences with homology to the TIR/NBD/LRR family of disease resistance genes. Four of these RLGs represent two potentially novel classes of disease resistance genes: TIR/NBD domains fused inframe to a putative defense-related protein (NtPRp27-like) and TIR domains fused inframe to soybean calmodulin Ca2+-binding domains. RT-PCR analyses using gene-specific primers allowed us to monitor the expression of individual genes in different tissues and developmental stages. Three genes appeared to be constitutively expressed, while three were differentially expressed. Analyses of the R-genes within this BAC suggest that R-gene evolution in soybean is a complex and dynamic process.


BMC Genomics ◽  
2011 ◽  
Vol 12 (1) ◽  
Author(s):  
Alessandra F Ribas ◽  
Alberto Cenci ◽  
Marie-Christine Combes ◽  
Hervé Etienne ◽  
Philippe Lashermes

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