Identification and mapping of a novel blackleg resistance locus LepR4 in the progenies from Brassica napus × B. rapa subsp. sylvestris

2012 ◽  
Vol 126 (2) ◽  
pp. 307-315 ◽  
Author(s):  
Fengqun Yu ◽  
Richard K. Gugel ◽  
H. Randy Kutcher ◽  
Gary Peng ◽  
S. Roger Rimmer
Keyword(s):  
Brassica Napus ◽  
Resistance Locus ◽  
Blackleg Resistance

Identification and mapping of a third blackleg resistance locus in Brassica napus derived from B. rapa subsp. sylvestris

Genome ◽  
2008 ◽  
Vol 51 (1) ◽  
pp. 64-72 ◽  
Author(s):  
Fengqun Yu ◽  
Derek J. Lydiate ◽  
S. Roger Rimmer
Keyword(s):  
Brassica Napus ◽  
Linkage Group ◽  
Resistance Gene ◽  
Leptosphaeria Maculans ◽  
Resistance Locus ◽  
Dominant Allele ◽  
Breeding Lines ◽  
Disease Reaction ◽  
Blackleg Resistance ◽  
Map Location

The spectrum of resistance to isolates of Leptosphaeria maculans and the map location of a new blackleg resistance gene found in the canola cultivar Brassica napus ‘Surpass 400’ are described. Two blackleg resistance genes, LepR1 and LepR2, from B. rapa subsp. sylvestris and introgressed in B. napus were identified previously. ‘Surpass 400’ also has blackleg resistance introgressed from B. rapa subsp. sylvestris. Using 31 diverse isolates of L. maculans, the disease reaction of ‘Surpass 400’ was compared with those of the resistant breeding lines AD9 (which contains LepR1), AD49 (which contains LepR2), and MC1-8 (which contains both LepR1 and LepR2). The disease reaction on ‘Surpass 400’ was different from those observed on AD9 and MC1-8, indicating that ‘Surpass 400’ carries neither LepR1 nor both LepR1 and LepR2 in combination. Disease reactions of ‘Surpass 400’ to most of the isolates tested were indistinguishable from those of AD49, which suggested ‘Surpass 400’ might contain LepR2 or a similar resistance gene. Classical genetic analysis of F1 and BC1 plants showed that a dominant allele conferred resistance to isolates of L. maculans in ‘Surpass 400’. The resistance gene, which mapped to B. napus linkage group N10 in an interval of 2.9 cM flanked by microsatellite markers sR12281a and sN2428Rb and 11.7 cM below LepR2, was designated LepR3. A 9 cM region of the B. napus genome containing LepR3 was found to be syntenic with a segment of Arabidopsis chromosome 5.

scholarly journals In-silico identification and differential expression of putative disease resistance-related genes within the collinear region of Brassica napus blackleg resistance locus LepR2’ in Brassica oleracea

2020 ◽  
Vol 61 (5) ◽  
pp. 879-890 ◽  
Author(s):  
Mohammad Rashed Hossain ◽  
Mostari Jahan Ferdous ◽  
Jong-In Park ◽  
Arif Hasan Khan Robin ◽  
Sathishkumar Natarajan ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Brassica Napus ◽  
Resistance Locus ◽  
R Gene ◽  
R Genes ◽  
Blackleg Resistance ◽  
B Genome ◽  
Expression Of Genes ◽  
C Genome ◽  
R Loci

Abstract Blackleg disease, caused by Leptosphaeria maculans, greatly affects the production of cabbage (Brassica oleracea). However, definitive R-gene(s) are yet to be identified in this crop. In contrast, a number of R-loci have been identified in A- or B-genome crops. Identification of few resistant cabbage genotypes indicates the presence of R-genes in this C-genome crop. High ancestral synteny between Brassica genomes suggests that the collinear regions of known A- or B-genome R-loci may also contain functional R-genes in the C-genome. Strong resistance was observed in the cotyledons of cabbage inbred line SCNU-98 against two L. maculans isolates, 03–02 s and 00–100 s. We investigated the collinear region of the Brassica napus blackleg resistance locus LepR2’ in B. oleracea since both isolates of L. maculans contain corresponding avirulence genes. The locus was collinear to a 5.8 Mbp genomic segment of B. oleracea chromosome C09 containing 13 genes that have putative disease resistance-related domains. High expression of genes Bo9g117290 and Bo9g111510 against isolate 00–100 s, and high expression of genes Bo9g126150 and Bo9g111490 against both isolates in the resistant-line SCNU-98 indicate their putative roles in blackleg resistance, which remained to be functionally verified. This work enhances our understanding of R-gene-mediated resistance to blackleg in cabbage.

scholarly journals Recent Findings Unravel Genes and Genetic Factors Underlying Leptosphaeria maculans Resistance in Brassica napus and Its Relatives

2020 ◽  
Vol 22 (1) ◽  
pp. 313
Author(s):  
Aldrin Y. Cantila ◽  
Nur Shuhadah Mohd Saad ◽  
Junrey C. Amas ◽  
David Edwards ◽  
Jacqueline Batley
Keyword(s):  
Brassica Napus ◽  
Genetic Factors ◽  
Quantitative Resistance ◽  
Genetic Resistance ◽  
Leptosphaeria Maculans ◽  
Gene Interaction ◽  
R Gene ◽  
R Genes ◽  
Blackleg Resistance ◽  
Avr Gene

Among the Brassica oilseeds, canola (Brassica napus) is the most economically significant globally. However, its production can be limited by blackleg disease, caused by the fungal pathogen Lepstosphaeria maculans. The deployment of resistance genes has been implemented as one of the key strategies to manage the disease. Genetic resistance against blackleg comes in two forms: qualitative resistance, controlled by a single, major resistance gene (R gene), and quantitative resistance (QR), controlled by numerous, small effect loci. R-gene-mediated blackleg resistance has been extensively studied, wherein several genomic regions harbouring R genes against L. maculans have been identified and three of these genes were cloned. These studies advance our understanding of the mechanism of R gene and pathogen avirulence (Avr) gene interaction. Notably, these studies revealed a more complex interaction than originally thought. Advances in genomics help unravel these complexities, providing insights into the genes and genetic factors towards improving blackleg resistance. Here, we aim to discuss the existing R-gene-mediated resistance, make a summary of candidate R genes against the disease, and emphasise the role of players involved in the pathogenicity and resistance. The comprehensive result will allow breeders to improve resistance to L. maculans, thereby increasing yield.

Development of an agronomically superior blackleg resistant canola cultivar in Brassica napus L. using doubled haploidy

1995 ◽  
Vol 75 (2) ◽  
pp. 437-439 ◽  
Author(s):  
G. R. Stringam ◽  
V. K. Bansal ◽  
M. R. Thiagarajah ◽  
D. F. Degenhardt ◽  
J. P. Tewari
Keyword(s):  
Brassica Napus ◽  
Doubled Haploid ◽  
Leptosphaeria Maculans ◽  
Resistance Breeding ◽  
Breeding Method ◽  
Brassica Napus L ◽  
Breeding Lines ◽  
University Of Alberta ◽  
Blackleg Resistance ◽  
The University

The doubled haploid breeding method and greenhouse screening using cotyledon bio-assay were successfully applied to transfer blackleg resistance from the Australian cultivar Maluka (Brassicas napus), into susceptible advanced B. napus lines from the University of Alberta. This approach for blackleg resistance breeding was effective and efficient as several superior blackleg resistant breeding lines were identified within 4 yr from the initial cross. One of these lines (91–21864NA) was entered in the 1993 trials of the Western Canada Canola/Rapeseed Recommending Committee. Key words: Blackleg resistance, Leptosphaeria maculans, doubled haploid, Brassica napus

scholarly journals The Brassica napus wall‐associated kinase‐like (WAKL) gene Rlm9 provides race‐specific blackleg resistance

2020 ◽  
Vol 104 (4) ◽  
pp. 892-900 ◽  
Author(s):  
Nicholas J. Larkan ◽  
Lisong Ma ◽  
Parham Haddadi ◽  
Miles Buchwaldt ◽  
Isobel A.P. Parkin ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Blackleg Resistance

Detecting and estimating segregation distortion and linkage between glufosinate tolerance and blackleg resistance in Brassica napus L.

Euphytica ◽  
2006 ◽  
Vol 148 (3) ◽  
pp. 217-225 ◽  
Author(s):  
Rong-Cai Yang ◽  
Mohan R. Thiagarajah ◽  
Vipan K. Bansal ◽  
Gary R. Stringam ◽  
M. Habibur Rahman
Keyword(s):  
Brassica Napus ◽  
Segregation Distortion ◽  
Brassica Napus L ◽  
Blackleg Resistance

Identification of two blackleg resistance genes and fine mapping of one of these two genes in a Brassica napus canola cultivar ‘Surpass 400’

2011 ◽  
Vol 122 (6) ◽  
pp. 1223-1231 ◽  
Author(s):  
Yunming Long ◽  
Zining Wang ◽  
Zudong Sun ◽  
Dilantha W. G. Fernando ◽  
Peter B. E. McVetty ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Resistance Genes ◽  
Fine Mapping ◽  
Blackleg Resistance

scholarly journals Complexities of Chromosome Landing in a Highly Duplicated Genome: Toward Map-Based Cloning of a Gene Controlling Blackleg Resistance in Brassica napus

Genetics ◽  
2005 ◽  
Vol 171 (4) ◽  
pp. 1977-1988 ◽  
Author(s):  
Reinhold Mayerhofer ◽  
Kris Wilde ◽  
Marion Mayerhofer ◽  
Derek Lydiate ◽  
Vipan K. Bansal ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Blackleg Resistance

Identification of two novel genes for blackleg resistance in Brassica napus

2005 ◽  
Vol 110 (5) ◽  
pp. 969-979 ◽  
Author(s):  
F. Yu ◽  
D. J. Lydiate ◽  
S. R. Rimmer
Keyword(s):  
Brassica Napus ◽  
Novel Genes ◽  
Blackleg Resistance
Sign in / Sign up

Export Citation Format

Share Document