Identification of putative quantitative trait loci associated with a flavonoid related to resistance to cabbage seedpod weevil (Ceutorhynchus obstrictus) in canola derived from an intergeneric cross, Sinapis alba × Brassica napus

AbstractIntrogression of cabbage seedpod weevil, Ceutorhynchus obstrictus (Marsham) (Coleoptera: Curculionidae), resistance from Sinapis alba L. to susceptible Brassica napus L. (Brassicaceae) has produced genetic lines resistant to the weevil in replicated field trials. In the current study, weevil feeding and oviposition on S. alba and on resistant novel lines developed by crossing S. alba × B. napus were less frequent than on susceptible germplasm. Development times were greater and biomass was less when larvae were reared on resistant lines or S. alba. Oocyte development was faster in post-diapause springtime adult female weevils caged on susceptible plants than in those on a resistant line, S. alba, or an early-season food host, Thlaspi arvense L (Brassicaceae). Our results suggest that antixenosis resistance and antibiosis resistance are expressed by resistant lines. These results and previous chemical analyses of these lines also suggest that resistance is potentially influenced by attractive and (or) feeding-stimulant effects of 2-phenylethyl glucosinolate and antifeedant or toxic effects of 1-methoxy-3-indolylmethyl glucosinolate.

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Comparative mapping of quantitative trait loci involved in heterosis for seedling and yield traits in oilseed rape (Brassica napus L.)

Theoretical and Applied Genetics ◽

10.1007/s00122-009-1133-z ◽

2009 ◽

Vol 120 (2) ◽

pp. 271-281 ◽

Cited By ~ 103

Author(s):

P. Basunanda ◽

M. Radoev ◽

W. Ecke ◽

W. Friedt ◽

H. C. Becker ◽

...

Keyword(s):

Brassica Napus ◽

Quantitative Trait Loci ◽

Oilseed Rape ◽

Quantitative Trait ◽

Comparative Mapping ◽

Yield Traits ◽

Brassica Napus L ◽

Trait Loci

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Linkage Analysis of Molecular Markers and Quantitative Trait Loci in Populations of Inbred Backcross Lines of Brassica napus L.

Genetics ◽

10.1093/genetics/153.2.949 ◽

1999 ◽

Vol 153 (2) ◽

pp. 949-964 ◽

Cited By ~ 2

Author(s):

David V Butruille ◽

Raymond P Guries ◽

Thomas C Osborn

Keyword(s):

Genetic Variation ◽

Brassica Napus ◽

Quantitative Trait Loci ◽

Qtl Analysis ◽

Quantitative Trait ◽

Agronomic Traits ◽

Pedigree Structure ◽

Inbred Backcross Lines ◽

Trait Loci ◽

Inbred Backcross

Abstract Backcross populations are often used to study quantitative trait loci (QTL) after they are initially discovered in balanced populations, such as F2, BC1, or recombinant inbreds. While the latter are more powerful for mapping marker loci, the former have the reduced background genetic variation necessary for more precise estimation of QTL effects. Many populations of inbred backcross lines (IBLs) have been developed in plant and animal systems to permit simultaneous study and dissection of quantitative genetic variation introgressed from one source to another. Such populations have a genetic structure that can be used for linkage estimation and discovery of QTL. In this study, four populations of IBLs of oilseed Brassica napus were developed and analyzed to map genomic regions from the donor parent (a winter-type cultivar) that affect agronomic traits in spring-type inbreds and hybrids. Restriction fragment length polymorphisms (RFLPs) identified among the IBLs were used to calculate two-point recombination fractions and LOD scores through grid searches. This information allowed the enrichment of a composite genetic map of B. napus with 72 new RFLP loci. The selfed and hybrid progenies of the IBLs were evaluated during two growing seasons for several agronomic traits. Both pedigree structure and map information were incorporated into the QTL analysis by using a regression approach. The number of QTL detected for each trait and the number of effective factors calculated by using biometrical methods were of similar magnitude. Populations of IBLs were shown to be valuable for both marker mapping and QTL analysis.

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