Abstract
Background: Thlaspi arvense Linn, belonging to the dicotyledonous cruciferous family, is distributed Europe and Asia. In this study, we evaluated for the first time the anti-inflammatory effects of Thlaspi arvense Linn on LPS-stimulated RAW264.7 macrophages, and explored the related mechanism.Methods: The extract was identified and quantified using the HPLC, NMR. The anti-inflammatory activities of crude extracts C11, C12, C13 were screened by xylene-induced ear swelling and carrageenan-induced foot swelling in mice. The inflammatory mediators, pro-inflammatory cytokines and TLR-4-mediated signals in LPS-stimulated RAW264.7 macrophages were determined using NO activity assay, MTS, ELISA and Western blot.Results:The extract of Thlaspi arvense Linn was found to enrich flavonoid(mainly Orientin,Isoorientin,Vitexin;,Isovitexin,Luteolin-7-O-β-D-glucoside,Apigenin-7-O-β-D-glucoside,Luteolin,Apigeni).5,7-dihydroxy-flavone-4′-(6′′-β-O-glucopyranoside)-β-O-D-glucopyranoside was novel, whereas isosapogenin and 8-methoxyvitexin were isolated for the first time from Thlaspi arvense Linn. The extract (flavonoid-enriched) inhibited xylene-induced ear swelling and carrageenan-induced foot swelling in mice. And suppressed LPS-induced overrelease of iNOS,TNF-α,COX-2 and IL-6 in RAW264.7 macrophages. The extract inhibited the inflammatory response through the signaling pathway mediated by TLR-4/NF- kappa B pathway and its downstream signals, IκB-α, NF-κB-P65 and IL-1β in LPS-stimulated macrophages.Conclusions: The present results demonstrate that the extract of Thlaspi arvense Linn inhibit inflammatory responses via the TLR-4/NF-KB-mediated signaling pathway.