RFLP analysis and genomic in situ hybridization (GISH) in somatic hybrids and their progeny between Lycopersicon esculentum and Solanum lycopersicoides

1998 ◽  
Vol 96 (6-7) ◽  
pp. 719-726 ◽  
Author(s):  
A. Escalante ◽  
S. Imanishi ◽  
M. Hossain ◽  
N. Ohmido ◽  
K. Fukui
Keyword(s):  
In Situ Hybridization ◽  
Lycopersicon Esculentum ◽  
Somatic Hybrids ◽  
Rflp Analysis ◽  
Genomic In Situ Hybridization ◽  
Solanum Lycopersicoides

Characterization by RFLP analysis and genomic in situ hybridization of a recombinant and a monosomic substitution plant derived from Hordeum vulgare L. × Hordeum bulbosum L. crosses

Genome ◽  
1997 ◽  
Vol 40 (2) ◽  
pp. 195-200 ◽  
Author(s):  
R. A. Pickering ◽  
A. M. Hill ◽  
R. G. Kynast
Keyword(s):  
In Situ Hybridization ◽  
Hordeum Vulgare ◽  
Rflp Analysis ◽  
Leaf Sheath ◽  
Genomic In Situ Hybridization ◽  
Barley Chromosome ◽  
Interspecific Crosses ◽  
Hordeum Bulbosum ◽  
Hordeum Vulgare L

Interspecific crosses in Hordeum have been made with the aim of transferring desirable traits, such as disease resistance, from a wild species, Hordeum bulbosum, into cultivated barley (Hordeum vulgare). Interspecific recombinants have previously been identified using several methods, but there are limitations with all the techniques. We improved our ability to characterize progeny from H. vulgare × H. bulbosum crosses by using genomic in situ hybridization (GISH). The plant material comprised a recombinant and a monosomic alien substitution plant derived from H. vulgare × H. bulbosum crosses. The recombinant possesses a pubescent leaf sheath conferred by a gene transferred from H. bulbosum into barley cultivar Golden Promise. The use of GISH on a plant homozygous for the pubescence gene confirmed the presence of H. bulbosum DNA located distally on two barley chromosomes and we mapped the introgression to barley chromosome 4HL using RFLP analysis. Furthermore, by means of an allelism test we found that the transferred gene for pubescence is allelic or closely linked to a gene for pubescence (Hs) located on barley chromosome 4HL. The presence of a single H. bulbosum chromosome in the monosomic substitution plant was confirmed by GISH. A distal introgression of H. bulbosum DNA was also observed on one barley chromosome, which was located on chromosome 3HL by RFLP analysis.Key words: Hordeum vulgare, Hordeum bulbosum, interspecific hybrid, gene introgression, genomic in situ hybridization.

Identification of alien chromosomes through GISH and RFLP analysis and the potential for establishing potato lines with monosomic additions of tomato chromosomes

Genome ◽  
1997 ◽  
Vol 40 (5) ◽  
pp. 666-673 ◽  
Author(s):  
F. Garriga-Calderé ◽  
D. J. Huigen ◽  
F. Filotico ◽  
E. Jacobsen ◽  
M. S. Ramanna
Keyword(s):  
In Situ Hybridization ◽  
Somatic Hybrids ◽  
Rflp Analysis ◽  
Chromosome Elimination ◽  
Variable Number ◽  
Substitution Lines ◽  
Chromosome Addition ◽  
Tomato Chromosome ◽  
Complete Series

To increase the potential for establishing a complete series of tomato chromosome addition–sbstitution lines in a potato background, six new BC1 progeny were produced. All of them originated from crosses between three different hexaploid potato (+) tomato fusion hybrids. Three different somatic hybrids, viz., C31-17-5, C31-17-24, and C31-17-51, were used as female parents, and four different tetraploids, viz., Katahdin, Frieslander, 6704-1, and AM66.42 were used as male parents. A characterisation of the genomes of the three fusion hybrids and the six BC1 progenies (6739, 2001, 2002, 2003, 2004, and 2005) through genomic in situ hybridization and restriction fragment length polymorphism (RFLP) analysis indicated that there was preferential tomato chromosome elimination in the fusion hybrids. Similar analyses of the six BC1 progeny indicated that a variable number of the alien tomato chromosomes (6–11) were present in individual plants. RFLP analysis using chromosome specific DNA probes indicated that BC1 progenies had retained all 12 tomato chromosomes, albeit in different individual plants. This means that the available BC1 progenies have the potential for establishing a complete series of tomato chromosome addition–substitution lines in a potato background.Key words: protoplast fusion hybrids, Solanum tuberosum, Lycopersicon esculentum, BC1 progeny, in situ hybridization, RFLP analysis.

Cytological characterization of potato - Solanum etuberosum somatic hybrids and their backcross progenies by genomic in situ hybridization

Genome ◽  
1999 ◽  
Vol 42 (5) ◽  
pp. 987-992 ◽  
Author(s):  
F Dong ◽  
R G Novy ◽  
J P Helgeson ◽  
J Jiang
Keyword(s):  
In Situ Hybridization ◽  
Dna Marker ◽  
Somatic Hybrids ◽  
Molecular Cytogenetics ◽  
Genomic In Situ Hybridization ◽  
Preferential Pairing ◽  
Potato Germplasm ◽  
Solanum Etuberosum

Four somatic hybrids derived from a diploid wild species Solanum etuberosum and a diploid tuber-bearing Solanum clone 463-4, together with five BC1 and three BC2 plants, were analyzed by genomic in situ hybridization (GISH). None of the four somatic hybrids had the expected chromosome constitutions, i.e., 24 chromosomes from each fusion parent. Either one chromosome from S. etuberosum or one from the potato parent 463-4 was lost in the hybrids. Three BC1 plants had exactly one set of S. etuberosum chromosomes. The other two BC1 plants either had one extra or one fewer S. etuberosum chromosome, possibly because their somatic hybrid parents had an extra or had lost one S. etuberosum chromosome. The presence of one set, or close to one set, of S. etuberosum chromosomes in all BC1 plants suggests a preferential pairing and segregation of the S. etuberosum chromosomes in the somatic hybrids. Two of the three BC2 plants had 52 chromosomes, deviating significantly from the expected chromosome number of 48. These results suggest poor pairing between S. etuberosum and S. tuberosum chromosomes in the BC1 plants. The present study demonstrates the importance of combining GISH and DNA marker analysis for a thorough characterization of potato germplasm containing chromosomes from different species.Key words: potato germplasm, Solanum etuberosum, molecular cytogenetics.

Genome organization of Brassica napus and Lesquerella fendleri and analysis of their somatic hybrids using genomic in situ hybridization

Genome ◽  
1998 ◽  
Vol 41 (5) ◽  
pp. 691-701 ◽  
Author(s):  
Marina Skarzhinskaya ◽  
Jan Fahleson ◽  
Kristina Glimelius ◽  
Armand Mouras
Keyword(s):  
In Situ Hybridization ◽  
Brassica Napus ◽  
Genome Organization ◽  
Somatic Hybrids ◽  
Genomic In Situ Hybridization ◽  
Lesquerella Fendleri

Detection of specific chromosome reduction in rice somatic hybrids with the A, B, and C genomes by multi-color genomic in situ hybridization

1998 ◽  
Vol 97 (7) ◽  
pp. 1013-1018 ◽  
Author(s):  
R. Shishido ◽  
S. Apisitwanich ◽  
N. Ohmido ◽  
Y. Okinaka ◽  
K. Mori ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Somatic Hybrids ◽  
Genomic In Situ Hybridization ◽  
Specific Chromosome

Characterization of euploid backcross progenies derived from interspecific hybrids between Oryza sativa and O. eichingeri by restriction fragment length polymorphism (RFLP) analysis and genomic in situ hybridization (GISH)

Genome ◽  
2001 ◽  
Vol 44 (1) ◽  
pp. 86-95 ◽  
Author(s):  
Huihuang Yan ◽  
Guoqing Liu ◽  
Zhukuan Cheng ◽  
Shaokai Min ◽  
Lihuang Zhu
Keyword(s):  
Oryza Sativa ◽  
In Situ Hybridization ◽  
Restriction Fragment Length Polymorphism ◽  
Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Brown Planthopper ◽  
Rflp Analysis ◽  
Genomic In Situ Hybridization ◽  
Restriction Fragment Length

Restriction fragment length polymorphism (RFLP) analysis and GISH (genomic in situ hybridization) were performed on euploid plants derived from crosses between Oryza sativa (2n = 24, AA) and two brown planthopper-resistant accessions of O. eichingeri (2n = 24, CC). After screening with 164 RFLP markers, 60 of the 67 euploid plants were identified as introgression lines, each carrying 1–6 small O. eichingeri segments integrated on chromosomes 1, 2, 6, or 10. In the somatic chromosome preparations of F1 hybrid, O. eichingeri chromosomes, fluorescing greenish-yellow in the sequential GISH, appeared to be longer and to contain more heterochromatin than O. sativa ones, and this karyotypic polymorphism can be used to detect some introgressed O. eichingeri segments in euploid plants. In addition, GISH identification presented direct evidence for the transfer of small segments from O. eichingeri to O. sativa chromosome(s) which were subsequently recognized according to their condensation pattern, arm ratio, and chromosome length. The present results would contribute to the molecular mapping and selection of O. eichingeri - derived brown planthopper-resistant gene and positive yield QTLs.Key words: Oryza sativa, Oryza eichingeri, introgression lines, RFLP, genomic in situ hybridization (GISH).

scholarly journals Genomic in situ hybridization (GISH) and RFLP analysis for the identification of alien chromosomes in the backcross progeny of potato (+) tomato fusion hybrids

Heredity ◽  
1995 ◽  
Vol 74 (3) ◽  
pp. 250-257 ◽  
Author(s):  
E Jacobsen ◽  
J H de Jong ◽  
S A Kamstra ◽  
P M M M van den Berg ◽  
M S Ramanna
Keyword(s):  
In Situ Hybridization ◽  
Backcross Progeny ◽  
Rflp Analysis ◽  
Genomic In Situ Hybridization
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