Random amplified polymorphic DNA (RAPD) analysis reveals genetic relationships among the annual Cicer species

1999 ◽  
Vol 98 (3-4) ◽  
pp. 657-663 ◽  
Author(s):  
F. Ahmad
Keyword(s):  
Rapd Analysis ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Cicer Species

Phylogenetic relationships of five morphological groups of hexaploid wheat (Triticum aestivum L. em Thell.) based on RAPD analysis

Genome ◽  
2000 ◽  
Vol 43 (4) ◽  
pp. 724-727 ◽  
Author(s):  
Wenguang Cao ◽  
G Scoles ◽  
P Hucl ◽  
R N Chibbar
Keyword(s):  
Hexaploid Wheat ◽  
Phylogenetic Relationships ◽  
Genetic Similarity ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Triticum Aestivum L ◽  
Similarity Coefficients ◽  
Morphological Classification ◽  
Wild Wheat

The genetic relationships among the five groups of hexaploid wheat: common, spelta, macha, vavilovii, and semi-wild wheat (SWW) are not clear. Random amplified polymorphic DNA (RAPD) analysis was used to assess phylogenetic relationships among these five morphological groups of hexaploid wheat. RAPD data were analyzed using the NTSYS-PC computer program to generate Jaccard genetic similarity coefficients. A dendrogram based on RAPD analysis grouped 15 accessions into five distinct clusters. These results are in agreement with those based on morphological classification, suggesting that common wheat is most closely related to SWW, followed by spelta, vavilovii, and macha.Key words: RAPD, macha, spelta, vavilovii, semi-wild wheat, phylogenetic relationships.

Genetic-Relationships as Assessed by Molecular Markers and Cross-Infection Among Strains of Colletotrichum gloeosporioides

1994 ◽  
Vol 42 (1) ◽  
pp. 9 ◽  
Author(s):  
HL Hayden ◽  
KG Pegg ◽  
EAB Aitken ◽  
JAG Irwin
Keyword(s):  
Rapd Markers ◽  
Colletotrichum Gloeosporioides ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Colletotrichum Acutatum ◽  
Fruit Species ◽  
Pathogenicity Tests ◽  
Colletotrichum Musae ◽  
Highly Virulent

Morphological characterisation allows isolates of Colletotrichum gloeosporioides, Colletotrichum musae and Colletotrichum acutatum to be identified only to species level. Pathogenicity tests and random amplified polymorphic DNA (RAPD) markers distinguished a mango biotype of C. gloeosporioides from eight other isolates of C gloeosporioides obtained from five different fruit species. Using these procedures, it was also possible to distinguish C. acutatum and C. musae both from each other, and from the C. gloeosporioides isolates. In cross-infectivity studies, isolates of C. gloeosporioides displayed a wide host range with the exception of isolates from mango, which were highly virulent on mango only. Teleomorphic isolates of C. gloeosporioides were clustered together by RAPD analysis. This work has demonstrated the existence of a biotype of C. gloeosporioides which shows specialisation to mango.

Application of the random amplified polymorphic DNA technique for the detection of polymorphism among wild and cultivated tetraploid wheats

Genome ◽  
1993 ◽  
Vol 36 (3) ◽  
pp. 602-609 ◽  
Author(s):  
Chandrashekhar P. Joshi ◽  
Henry T. Nguyen
Keyword(s):  
Molecular Markers ◽  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Triticum Turgidum ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Emmer Wheat ◽  
Tetraploid Wheats

Development of a high-density genetic linkage map of cultivated wheats using conventional molecular markers has lagged behind the other major food crops such as rice and tomato because of the large genome size and limited levels of genetic polymorphisms. Recently, random amplified polymorphic DNAs (RAPDs) have been suggested to provide an alternative to visualize more polymorphism. For the construction of a genetic linkage map in tetraploid wheats, one can use a strategy of intersubspecific crosses between the most dissimilar wild and cultivated tetraploid wheats that are easy to hybridize and result in fertile progeny. An assessment of the level of RAPDs among different accessions and varieties of wild and cultivated tetraploid wheats is required to fulfill this objective. We present here the data obtained using RAPD analysis of 40 primers in 20 accessions of wild tetraploid emmer wheats (Triticum turgidum L. ssp. dicoccoides) and 10 genotypes of cultivated tetraploid durum wheats (Triticum turgidum L. ssp. durum) selected from geographically diverse locations. We have observed a higher level of polymorphism among different accessions of wild emmer wheat from Israel, Turkey, and Jordan than the group of cultivated American, Turkish, and Syrian durum wheats. These data have been used to generate a dendrogram suggesting the genetic relationships among these genotypes, and the most dissimilar genotypes are identified for future mapping and gene tagging work.Key words: durum wheat, emmer wheat, genetic similarity, molecular markers, RAPD analysis.

scholarly journals Identification of the Edible Fig `Bianco del Cilento' by Random Amplified Polymorphic DNA Analysis

HortScience ◽  
1999 ◽  
Vol 34 (7) ◽  
pp. 1263-1265 ◽  
Author(s):  
U. Galderisi ◽  
M. Cipollaro ◽  
G. Di Bernardo ◽  
L. De Masi ◽  
G. Galano ◽  
...  
Keyword(s):  
Dna Analysis ◽  
Southern Italy ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Accurate Identification ◽  
Banding Patterns ◽  
Campania Region ◽  
Quality Of Products

Random amplified polymorphic DNA (RAPD) analysis is currently used to estimate genetic relationships in plants. We have used RAPD analysis to distinguish six different cultivars of Ficus carica, and several of their clones, that are widespread in the Campania Region of Southern Italy. Among these cultivars, `Bianco del Cilento' has unique characteristics, and is particularly useful for drying and for the manufacture of syrups. The protection of this cultivar is important to the Campania Region. We have utilized molecular markers to allow accurate identification of this cultivar, making it possible to control the quality of products and prevent fraudulent commerce. DNA was extracted from leaves and amplified by PCR using random oligonucleotide primers. The amplification patterns obtained with five decamer primers were useful for distinguishing all six cultivars analyzed. `Bianco del Cilento' was identified by two primers. The banding patterns were scored and used in similarity value calculations to estimate genetic relationships.

Genetic Similarity Among Broccoli Inbreds “Essentially Derived” from Commercial Cultivars

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 546e-546 ◽  
Author(s):  
Mark W. Farnham ◽  
Jiang Lu ◽  
Julie M. Villand
Keyword(s):  
Rapd Markers ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Doubled Haploids ◽  
Threshold Level ◽  
Pedigree Information ◽  
Dimensional Scaling ◽  
Similarity Indices ◽  
Related Group

DNA markers can assess close genetic relationships between individuals of a crop as when one variety is developed (i.e., “essentially derived”) from another. An acceptable threshold, based on empirical results, should be established for a crop to indicate what constitutes an “essentially derived” variety in the absence of clear pedigree information. Empirical data could help settle infringements of intellectual property rights, but appropriate data are not being generated for most crops. Thus, our objectives were to characterize genetic relationships among broccoli varieties “essentially derived” from known parents using random amplified polymorphic DNA (RAPD) markers as a measure of genotype and to provide an empirical basis for threshold levels in this crop. Six F1 broccoli hybrids and three inbred lines (doubled-haploids) developed from each of the hybrids (24 entries) were evaluated by RAPD analysis. RAPD assays were conducted using 23 different oligonucleotide 10-mers. Of 179 RAPD bands scored, 94 were polymorphic among the entries. Similarity indices were computed from RAPD data, and a multi-dimensional scaling (MDS) plot was constructed. Similarity indices for all pairwise comparisons ranged from 0.40 to 0.90. `High Sierra' and it's derived lines were the most closely related group with indices from 0.81 to 0.90. With `High Sierra', `Sultan', and `Marathon', the three derived lines were more closely related to their respective parental hybrids than were any other entries. The hybrids `Futura', `Everest', and `Viking' were more genetically similar to other entries than to their derived lines. A threshold level based on data from `High Sierra', `Marathon', `Sultan', and their derived lines would not identify “essentially derived” lines developed from other hybrids.

RAPD variation in populations of Cardamine lilacina (Brassicaceae)

1996 ◽  
Vol 9 (3) ◽  
pp. 291 ◽  
Author(s):  
MF Nolan ◽  
ML Skotnicki ◽  
AJ Gibbs
Keyword(s):  
Population Structure ◽  
Host Plant ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Heterogeneous Population ◽  
Natural Host ◽  
Endemic Plant ◽  
Plant Variation ◽  
Recent Immigrant

Random amplified polymorphic DNA (RAPD) analysis of DNA samples clearly distinguished between Cardantine hirsuta L. and two variants of Cardamine lilacina Hook. found in Australia. Cardarnine hirsuta, a recent immigrant, was less variable than C. lilacina, robust alpine snow-patch variant (C. lilacina-RASPV), which is an endemic plant that grows in three distinct regions in the Kosciusko alpine area and C. lilacina, intermedia variant (C. lilacina-IV), which is an endemic plant that is widespread in the same area. Comparison of RAPDs showed that, in the populations of C. lilacina-RASPV, some plants found near each other were closely related, but there was no detectable largerscale population structure. It is most likely that the present populations of C. lilacina-RASPV are relics of a single larger heterogeneous population. Cardarnine lilacina-RASPV is the only known natural host of turnip yellow mosaic tymovirus in Australia. Although in this study RAPD analysis did not indicate whether the host plant variation is influencing the variability of populations of this virus it did provide an understanding of the genetic relationships in these populations of C. lilacina.

scholarly journals The Use of ISSR and RAPD Markers for Genetic Diversity among South Tunisian Barley

ISRN Agronomy ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Ferdaous Guasmi ◽  
Walid Elfalleh ◽  
Hédia Hannachi ◽  
Khadija Fères ◽  
Leila Touil ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Resolving Power ◽  
Poor Correlation ◽  
Rapd And Issr ◽  
South Tunisia ◽  
Issr Primers

Random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) were assayed to determine the genetic diversity of 80 barley specimens from South Tunisia. The ISSR primers showed variation in the percentage of polymorphism, band informativeness (Ib), and resolving power (Rp). The percentage of polymorphism is 66.67%, the average Ib ranged from 0.24 to 0.39, while Rp ranged from 0.74 to 1.16. In RAPD analysis, three primers yielded a total of 17 scorable bands, which are all polymorphic. The three polymorphic primers exhibited variation with regard to average band informativeness (AvIb) and resolving power (Rp). RAPD and ISSR marker systems were found to be useful for the genetic diversity among the barley specimens. The two dendrograms obtained through these markers show different clustering of 80 barely specimens, but we noted that some clusters were similar in some cases. A poor correlation () was found between both sets of genetic similarity data, suggesting that both sets of markers revealed unrelated estimates of genetic relationships. Therefore, the ISSR and RAPD molecular markers show two genetic grouping of studied barely specimens.

scholarly journals SNP and SCAR Markers for Specific Discrimination of Antler-Shaped Ganoderma lucidum

2019 ◽  
Vol 7 (1) ◽  
pp. 12 ◽  
Author(s):  
O-Chul Kwon ◽  
Chang-Soo Lee ◽  
Young-Jin Park
Keyword(s):  
Ganoderma Lucidum ◽  
Gene Sequence ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Morphological Characteristics ◽  
Pcr Analysis ◽  
Scar Markers ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Specific Fragment

In this study we identified single nucleotide polymorphism (SNP) and sequence characteristic amplification region (SCAR) markers for specific identification of antler-shaped Ganoderma lucidum strains. When the partial mitochondrial SSU rDNA gene sequence of various antler- and kidney-shaped G. lucidum strains were analyzed and aligned, an SNP was found only in the antler-shaped G. lucidum strain at position 456 bp. In addition, this SNP of antler-shaped strains was digested by HinfI restriction enzyme. We further analyzed the polymorphism of various G. lucidum strains by random amplified polymorphic DNA (RAPD) analysis. In RAPD analysis, we isolated and sequenced a fragment, specific for antler-shaped G. lucidum strains. Based on this specific fragment sequence, two sets of specific primer pairs for antler-shaped G. lucidum strains were designed. PCR analysis revealed that two specific bands were observed only from antler-shaped strains. These two molecular markers will be helpful for identification of morphological characteristics of G. lucidum.

Random amplified polymorphic DNA (RAPD) analysis for the verification of hybridity in interspecific crosses of Alstroemeria

2000 ◽  
Vol 119 (5) ◽  
pp. 443-445 ◽  
Author(s):  
L. de Benedetti ◽  
G. Burchi ◽  
A. Mercuri ◽  
N. Pecchioni ◽  
P. Faccioli ◽  
...  
Keyword(s):  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Interspecific Crosses

scholarly journals Isolates of Uromyces appendiculatus with Specific Virulence to Landraces of Phaseolus vulgaris of Andean Origin

Plant Disease ◽  
1999 ◽  
Vol 83 (2) ◽  
pp. 108-113 ◽  
Author(s):  
Craig M. Sandlin ◽  
James R. Steadman ◽  
Carlos M. Araya ◽  
Dermot P. Coyne
Keyword(s):  
Phaseolus Vulgaris ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Rust Fungus ◽  
Distinct Group ◽  
Uromyces Appendiculatus ◽  
Bean Rust ◽  
Banding Patterns ◽  
Highly Virulent ◽  
Specific Virulence

Five isolates of the bean rust fungus Uromyces appendiculatus were shown to be specifically virulent on bean genotypes of Andean origin. This specificity was demonstrated by the virulence of five pairs of isolates on a differential set of 30 Phaseolus vulgaris landraces. Each isolate pair was from a different country in the Americas and consisted of one Andean-specific isolate and one nonspecific isolate. Of the differential P. vulgaris landraces, 15 were of Middle American origin and 15 were of Andean origin. The Andean-specific rust isolates were highly virulent on Andean landraces but not on landraces of Middle American origin. Rust isolates with virulence to Middle American landraces were also generally virulent on Andean material; no truly Middle American-specific isolates were found. Random amplified polymorphic DNA (RAPD) analysis of the rust isolates also distinguished the two groups. Four of the Andean-specific rust isolates formed a distinct group compared to four of the nonspecific isolates. Two of the isolates, one from each of the two virulence groups, had intermediate RAPD banding patterns, suggesting that plasmagomy but not karyogamy occurred between isolates of the two groups.

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