Cell patterning via optimized dielectrophoretic force within hexagonal electrodes in vitro for skin tissue engineering

2019 ◽  
Vol 105 (12) ◽  
pp. 4899-4907 ◽  
Author(s):  
Zhijie Huan ◽  
Weicheng Ma ◽  
Min Xu ◽  
Zhixiong Zhong ◽  
Xiangpeng Li ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Patterning ◽  
Skin Tissue ◽  
Dielectrophoretic Force ◽  
Skin Tissue Engineering

Needleless electrospinning of PVP/PGS fibrous scaffolds for skin tissue engineering applications

2018 ◽  
Author(s):  
Antonios Keirouz ◽  
Giuseppino Fortunato ◽  
Anthony Callanan ◽  
Norbert Radacsi
Keyword(s):  
Tissue Engineering ◽  
Tensile Modulus ◽  
Dermal Fibroblasts ◽  
Skin Tissue ◽  
Skin Substitute ◽  
Electrospinning Technique ◽  
Skin Tissue Engineering ◽  
Needleless Electrospinning ◽  
High Concentrations

Scaffolds and implants used for tissue engineering need to be adapted for their mechanical properties with respect to their environment within the human body. Therefore, a novel composite for skin tissue engineering is presented by use of blends of Poly(vinylpyrrolidone) (PVP) and Poly(glycerol sebacate) (PGS) were fabricated via the needleless electrospinning technique. The formed PGS/PVP blends were morphologically, thermochemically and mechanically characterized. The morphology of the developed fibers related to the concentration of PGS, with high concentrations of PGS merging the fibers together plasticizing the scaffold. The tensile modulus appeared to be affected by the concentration of PGS within the blends, with an apparent decrease in the elastic modulus of the electrospun mats and an exponential increase of the elongation at break. Ultraviolet (UV) crosslinking of PGS/PVP significantly decreased and stabilized the wettability of the formed fiber mats, as indicated by contact angle measurements. In vitro examination showed good viability and proliferation of human dermal fibroblasts over the period of a week. The present findings provide important insights for tuning the elastic properties of electrospun material by incorporating this unique elastomer, as a promising future candidate for skin substitute constructs.

scholarly journals 075 Generation and quantification of oxidized squalene to develop an acne testing model in vitro based on skin tissue engineering

2016 ◽  
Vol 136 (9) ◽  
pp. S173 ◽  
Author(s):  
N. Esselin ◽  
C. Capallere ◽  
C. Meyrignac ◽  
C. Plaza ◽  
C. Coquet ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Skin Tissue ◽  
Testing Model ◽  
Skin Tissue Engineering

WITHDRAWN: Skin Tissue Engineering—In Vivo and In Vitro Applications

2012 ◽  
Vol 39 (1) ◽  
pp. 33-58 ◽  
Author(s):  
Florian Groeber ◽  
Monika Holeiter ◽  
Martina Hampel ◽  
Svenja Hinderer ◽  
Katja Schenke-Layland
Keyword(s):  
Tissue Engineering ◽  
Skin Tissue ◽  
Skin Tissue Engineering

scholarly journals Poly(L-lactide-co-glycolide) thin films can act as autologous cell carriers for skin tissue engineering

2014 ◽  
Vol 19 (2) ◽  
Author(s):  
Aleksandra Zuber ◽  
Julia Borowczyk ◽  
Eliza Zimolag ◽  
Malgorzata Krok ◽  
Zbigniew Madeja ◽  
...  
Keyword(s):  
Thin Films ◽  
Tissue Engineering ◽  
Human Skin ◽  
Skin Tissue ◽  
Aliphatic Polyesters ◽  
Skin Tissue Engineering ◽  
Cytoskeleton Organization ◽  
Skin Keratinocytes ◽  
Cell Carriers

AbstractDegradable aliphatic polyesters such as polylactides, polyglycolides and their copolymers are used in several biomedical and pharmaceutical applications. We analyzed the influence of poly(L-lactide-co-glycolide) (PLGA) thin films on the adhesion, proliferation, motility and differentiation of primary human skin keratinocytes and fibroblasts in the context of their potential use as cell carriers for skin tissue engineering. We did not observe visible differences in the morphology, focal contact appearance, or actin cytoskeleton organization of skin cells cultured on PLGA films compared to those cultured under control conditions. Moreover, we did not detect biologically significant differences in proliferative activity, migration parameters, level of differentiation, or expression of vinculin when the cells were cultured on PLGA films and tissue culture polystyrene. Our results indicate that PLGA films do not affect the basic functions of primary human skin keratinocytes and fibroblasts and thus show acceptable biocompatibility in vitro, paving the way for their use as biomaterials for skin tissue engineering.

Efficient in vitro transduction of epithelial cells and keratinocytes with improved adenoviral gene transfer for the application in skin tissue engineering

2002 ◽  
Vol 9 (2-4) ◽  
pp. 323-329 ◽  
Author(s):  
Cornelia Doebis ◽  
Thomas Ritter ◽  
Christine Brandt ◽  
Bernd Schönberger ◽  
Hans-Dieter Volk ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Gene Transfer ◽  
Epithelial Cells ◽  
Skin Tissue ◽  
Adenoviral Gene Transfer ◽  
Skin Tissue Engineering

In vitro characterisation of 3D printed platelet lysate-based bioink for potential application in skin tissue engineering

2021 ◽  
Vol 123 ◽  
pp. 286-297
Author(s):  
Luciana Y. Daikuara ◽  
Zhilian Yue ◽  
Danielle Skropeta ◽  
Gordon G. Wallace
Keyword(s):  
Tissue Engineering ◽  
Potential Application ◽  
Platelet Lysate ◽  
Skin Tissue ◽  
Skin Tissue Engineering ◽  
3D Printed
Sign in / Sign up

Export Citation Format

Share Document