Cloning and characterization of an extracellular serine protease from the nematode-trapping fungus Arthrobotrys conoides

2007 ◽  
Vol 188 (2) ◽  
pp. 167-174 ◽  
Author(s):  
Jinkui Yang ◽  
Juan Li ◽  
Lianming Liang ◽  
Baoyu Tian ◽  
Ying Zhang ◽  
...  
Keyword(s):  
Serine Protease ◽  
Arthrobotrys Conoides ◽  
Extracellular Serine Protease

Molecular and biochemical characterization of an extracellular serine-protease from Vibrio metschnikovii J1

2009 ◽  
Vol 36 (7) ◽  
pp. 939-948 ◽  
Author(s):  
Kemel Jellouli ◽  
Ali Bougatef ◽  
Laila Manni ◽  
Rym Agrebi ◽  
Rayda Siala ◽  
...  
Keyword(s):  
Serine Protease ◽  
Biochemical Characterization ◽  
Vibrio Metschnikovii ◽  
Extracellular Serine Protease

Purification and biochemical characterization of the haloalkaliphilic archaeonNatronococcus occultus extracellular serine protease

2001 ◽  
Vol 41 (6) ◽  
pp. 375-383 ◽  
Author(s):  
Claudia Alicia Studdert ◽  
Maria Karina Herrera Seitz ◽  
Maria Ines Plasencia Gil ◽  
Jorge Julian Sanchez ◽  
Rosana Esther de Castro
Keyword(s):  
Serine Protease ◽  
Biochemical Characterization ◽  
Extracellular Serine Protease

scholarly journals Cloning and characterization of the gene for an additional extracellular serine protease of Bacillus subtilis.

1991 ◽  
Vol 173 (21) ◽  
pp. 6889-6895 ◽  
Author(s):  
A Sloma ◽  
G A Rufo ◽  
K A Theriault ◽  
M Dwyer ◽  
S W Wilson ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Serine Protease ◽  
Extracellular Serine Protease

scholarly journals Purification and characterization of a thermostable, haloalkaliphilic extracellular serine protease from the extreme halophilic archaeonHalogeometricum borinquensestrain TSS101

Archaea ◽  
2006 ◽  
Vol 2 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Malashetty Vidyasagar ◽  
S. Prakash ◽  
Carol Litchfield ◽  
K. Sreeramulu
Keyword(s):  
Serine Protease ◽  
Cetyltrimethylammonium Bromide ◽  
High Thermal Stability ◽  
Purification And Characterization ◽  
Stationary Phase Culture ◽  
Phase Culture ◽  
The Stability ◽  
Ph Range ◽  
Extracellular Serine Protease

A novel haloalkaliphilic, thermostable serine protease was purified from the extreme halophilic archaeon,Halogeometricum borinquensestrain TSS101. The protease was isolated from a stationary phase culture, purified 116-fold with 18% yield and characterized biochemically. The molecular mass of the purified enzyme was estimated to be 86 kDa. The enzyme showed the highest activity at 60 °C and pH 10.0 in 20% NaCl. The enzyme had high activity over the pH range from 6.0 to 10.0. Enzymatic activity was strongly inhibited by 1 mM phenyl methylsulfonyl fluoride, but activity was increased 59% by 0.1% cetyltrimethylammonium bromide. The enzyme exhibited relatively high thermal stability, retaining 80% of its activity after 1 h at 90 °C. Thermostability increased in the presence of Ca2+. The stability of the enzyme was maintained in 10% sucrose and in the absence of NaCl.

Characterization of an Extracellular Serine Protease Gene from the Nematophagous Fungus Lecanicillium psalliotae

2005 ◽  
Vol 27 (17) ◽  
pp. 1329-1334 ◽  
Author(s):  
Jinkui Yang ◽  
Xiaowei Huang ◽  
Baoyu Tian ◽  
Hui Sun ◽  
Junxin Duan ◽  
...  
Keyword(s):  
Serine Protease ◽  
Nematophagous Fungus ◽  
Protease Gene ◽  
Lecanicillium Psalliotae ◽  
Serine Protease Gene ◽  
Extracellular Serine Protease
Sign in / Sign up

Export Citation Format

Share Document