Action mechanism of small and large molecule surfactant-based clove oil nanoemulsions against food-borne pathogens and real-time detection of their subpopulations

2014 ◽  
Vol 197 (1) ◽  
pp. 35-45 ◽  
Author(s):  
Hamid Majeed ◽  
John Antoniou ◽  
Charles F. Shoemaker ◽  
Zhong Fang
Keyword(s):  
Real Time ◽  
Large Molecule ◽  
Clove Oil ◽  
Action Mechanism ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Real Time Detection

Real-time PCR based detection of a panel of food-borne pathogens on a centrifugal microfluidic “LabDisk” with on-disk quality controls and standards for quantification

2014 ◽  
Vol 6 (7) ◽  
pp. 2038 ◽  
Author(s):  
Oliver Strohmeier ◽  
Nico Marquart ◽  
Daniel Mark ◽  
Günter Roth ◽  
Roland Zengerle ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Quality Controls ◽  
Food Borne Pathogens ◽  
Food Borne

scholarly journals Development of Simple Multiplex Real-Time PCR Assays for Foodborne Pathogens Detection and Identification On Lightcycler

2017 ◽  
Vol 40 (1) ◽  
pp. 53-58 ◽  
Author(s):  
Avo Karus ◽  
Fabrizio Ceciliani ◽  
Armand Sanches Bonastre ◽  
Virge Karus
Keyword(s):  
Real Time ◽  
Foodborne Pathogens ◽  
Real Time Pcr ◽  
Simultaneous Detection ◽  
Melting Curve ◽  
Food Contamination ◽  
Shigella Boydii ◽  
Food Borne Pathogens ◽  
Detection And Identification ◽  
Food Borne

Abstract Most acute intestinal diseases are caused by food-borne pathogens. A fast and simple real-time PCR-based procedure for simultaneous detection of food contamination by any of the five food-borne pathogens: Campylobacter jejuni, Mycobacterium bovis, Enterobacter sakazaki, Shigella boydii, Clostridium perfrigens using multiplex EvaGreen real-time PCR for LightCycler was developed and evaluated. Real-time qPCR showed excellent sensitivity. Tm calling and Melting Curve Genotyping (MCG) were used for analysis of PCR product melting curves. The Melting Curve Genotyping option showed good performance for discrimination of positive samples containing DNA of single pathogen or pathogen mixtures from negative samples.

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