scholarly journals Analysis of paralytic shellfish toxins using high-field asymmetric waveform ion mobility spectrometry with liquid chromatography-mass spectrometry

2015 ◽  
Vol 407 (9) ◽  
pp. 2473-2484 ◽  
Author(s):  
Daniel G. Beach ◽  
Jeremy E. Melanson ◽  
Randy W. Purves
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
High Field ◽  
Asymmetric Waveform ◽  
Liquid Chromatography Mass Spectrometry ◽  
Shellfish Toxins ◽  
Paralytic Shellfish Toxins ◽  
Paralytic Shellfish

scholarly journals Semiquantitation of Paralytic Shellfish Toxins by Hydrophilic Interaction Liquid Chromatography-Mass Spectrometry Using Relative Molar Response Factors

Toxins ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 398
Author(s):  
Jiangbing Qiu ◽  
Elliott J. Wright ◽  
Krista Thomas ◽  
Aifeng Li ◽  
Pearse McCarron ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Electrospray Ionization ◽  
Liquid Chromatography Mass Spectrometry ◽  
Response Factors ◽  
Shellfish Toxins ◽  
Paralytic Shellfish Toxins ◽  
Chromatography Mass Spectrometry ◽  
Paralytic Shellfish ◽  
Relative Molar Response

Paralytic shellfish toxins (PSTs) are a complex class of analogs of the potent neurotoxin saxitoxin (STX). Since calibration standards are not available for many PSTs, including C-11 hydroxyl analogs called M-toxins, accurate quantitation by liquid chromatography–mass spectrometry (LC-MS) can be challenging. In the absence of standards, PSTs are often semiquantitated using standards of a different analog (e.g., STX), an approach with a high degree of uncertainty due to the highly variable sensitivity between analytes in electrospray ionization. Here, relative molar response factors (RMRs) were investigated for a broad range of PSTs using common LC-MS approaches in order to improve the quantitation of PSTs for which standards are unavailable. First, several M-toxins (M1-M6, M9 and dcM6) were semipurified from shellfish using preparative gel filtration chromatography and quantitated using LC-charged aerosol detection (LC-CAD). The RMRs of PST certified reference materials (CRMs) and M-toxins were then determined using selective reaction monitoring LC-MS/MS and full scan LC-high-resolution MS (LC-HRMS) methods in positive and negative electrospray ionization. In general, RMRs for PSTs with similar chemical structures were comparable, but varied significantly between subclasses, with M-toxins showing the lowest sensitivity. For example, STX showed a greater than 50-fold higher RMR than M4 and M6 by LC-HRMS. The MS instrument, scan mode and polarity also had significant impacts on RMRs and should be carefully considered when semiquantitating PSTs by LC-MS. As a demonstration of their utility, the RMRs determined were applied to the semiquantitation of PSTs in contaminated mussels, showing good agreement with results from calibration with CRMs.

Calculation of ion mobilities from electrospray ionization high-field asymmetric waveform ion mobility spectrometry mass spectrometry

2001 ◽  
Vol 114 (23) ◽  
pp. 10270-10277 ◽  
Author(s):  
Roger Guevremont ◽  
David A. Barnett ◽  
Randy W. Purves ◽  
Larry A. Viehland
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
High Field ◽  
Asymmetric Waveform
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