scholarly journals Preparation and characterization of large-format macroporous cryogel disks for use in affinity chromatography and biotechnological applications

2018 ◽  
Vol 410 (29) ◽  
pp. 7765-7771
Author(s):  
Wim Noppe ◽  
Roxanne Cordier ◽  
Pieter Baatsen ◽  
Hans Deckmyn
Keyword(s):  
Affinity Chromatography ◽  
Biotechnological Applications ◽  
Large Format

The Immunological Characterization of Human Antibodies to Factor VIII Isolated by Immuno-Affinity Chromatography

1981 ◽  
Vol 45 (01) ◽  
pp. 060-064 ◽  
Author(s):  
M L Kavanagh ◽  
C N Wood ◽  
J F Davidson
Keyword(s):  
Factor Viii ◽  
Affinity Chromatography ◽  
Gel Filtration ◽  
Immunological Characterization ◽  
Human Antibodies ◽  
Heavy Chains ◽  
Light Chain Type ◽  
Antibody Preparations ◽  
Chain Type

SummaryNine human antibodies to factor VIII were isolated from haemophilic plasmas by affinity chromatography and gel filtration and six were subsequently subjected to immunological characterization. Three partially purified preparations were similarly characterized. Eight of the antibodies were characterized as being exclusively IgG and one preparation was found to contain IgM. Seven of the antibodies contained only a single light chain type, four being of type lambda and three of type kappa. Two antibody preparations contained both kappa and lambda light chains. In four of the preparations, only a single heavy chain sub-class could be demonstrated, three of IgG3 and one of IgG4. Of the remainder, three were a mixture of IgG3 and IgG4 sub-classes and one contained both IgG2 and IgG4. IgG sub-classification could not be achieved with the IgM-containing preparation. These results demonstrate a restricted heterogeneity of light and heavy chains in human antibodies to factor VIII.

scholarly journals CHARACTERIZATION OF MODERN CCD AND CMOS SENSORS FOR SKY SURVEYS

2021 ◽  
Vol 53 ◽  
pp. 190-197
Author(s):  
S. Karpov ◽  
A. Christov ◽  
A. Bajat ◽  
R. Cunniffe ◽  
M. Prouza
Keyword(s):  
Laboratory Testing ◽  
Frame Rate ◽  
Wide Field ◽  
Large Format ◽  
Ccd Cameras ◽  
Sky Survey ◽  
Monitoring Applications ◽  
Cmos Sensors ◽  
Robotic Telescopes

Here we review the efforts we take in a newly established laboratory inside Institute of Physics in Prague in order to characterize modern large-format CCD and CMOS sensors for sky survey applications. While the laboratory is primarily established in order to participate in low-level CCD sensor characterization for LSST project, we also managed to perform a thorough laboratory testing of recently released Andor Marana sCMOS (which is especially interesting for wide-field sky monitoring applications due to its large format, backilluminated design, high achievable frame rate and low read-out noise), as well as detailed measurements of response non-linearity of Moravian Instruments G4-16000 CCD cameras (based on large-format Kodak KAF-16803 chip) used in several robotic telescopes. We briefly review the results acquired on these cameras, as well as hardware and software we developed for the laboratory.

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