Improvement of Functional Properties of Jack Bean (Canavalia ensiformis) Flour by Germination and Its Relation to Amino Acids Profile

Jack bean as a source of vegetable protein had not been popular. Seed germination had been known to improve its nutritional quality, especially protein and amino acid profile. This study determined the effect of germination on the color, beany flavor, protein content, functional properties, and amino acid profile of jack bean flour. A complete randomized design was used for this experiment. Germination was carried out for 0, 24, 48, and 72 hours. The seed (control) and germinated jack bean flours were analyzed for oil absorption, water absorption, emulsifying and foaming capacities, as well as the soluble protein content to determine the best germination time. Furthermore, the amino acid profile of the jack bean flour produced from the best germination time was analyzed. The results of this study indicated that the total and soluble protein of the seed and germinated jack bean seeds for 0, 24, 48, 60, and 72 hours were 23.30 and 5.95; 22.61 and 7.61; 21.18 and 10.68; 23.26 and 10.22; 23.98 and 10.81%, respectively. Germination of jack bean improved the functional properties. A germination time of 72 hours increased the oil capacity, water absorption capacity, foaming capacity and decreased the emulsion capacity significantly. The hydrophilic and hydrophobic amino acids of the germinated jack bean flour increased to 3.21 and 2.12% of the seed flour, respectively. The increase of the foaming capacity was related to the increase in hydrophobic amino acids of germinated jack bean flour compared to seed flours, that were glycine 1.23 and 1.01; alanine 1.29 and 1.01; valine 1.16 and 1.00; leucine 1.84 and 1.09%, respectively. Germination of jack bean for 72 hours increased significantly the essential amino acids, namely: leucine, lysine, and valine.

Synthesis, Crystal Structures and Urease Inhibition of 4-Bromo-N’-(1-(pyridin-2-yl)ethylidene)benzohydrazide and Its Dinuclear Copper(II) Complex

A new dinuclear copper(II) complex [Cu2(μ-Br)2L2] · 0.5 MeOH with the benzohydrazone ligand 4-bromo-N’-(1-(pyridin-2-yl)ethylidene)benzohydrazide (HL) has been synthesized and characterized by elemental analysis, IR and UV-Vis spectroscopic studies. Single crystal structures of the complex and the benzohydrazone compound were studied. The Cu atoms in the complex are coordinated by two benzohydrazone ligands and two Br bridging groups, forming square pyramidal coordination. The complex has good inhibitory activity on Jack bean urease, with IC50 value of 1.38 μmol L-1.

Influence of Moisture and Temperature on the Thermal Properties of Jack Bean Seeds (Canavalia ensiformis)

The thermal properties (specific heat capacity, thermal conductivity, and thermal diffusivity) of jack bean seed (Canavalia ensiformis) were determined for usage in designing the equipment necessary for thermal processes. These thermal properties were determined at 5, 10, 15, 20, and 25 % moisture contents (wb) and temperatures at 30, 40, and 50oC using the KD2 Pro thermal analyzer. Results showed that the specific heat capacity ranged from 1.55 to 2.47 kJ/kgK, 1.26 to 1.84 kJ/kgK and 1.32 to 1.99 kJ/kgK; thermal conductivity 0.21 to 0.47 W/mK, 0.34 to 0.52 W/mK, and 0.26 to 0.60 W/mK and thermal diffusivity 0.25 to 0.41 x 10-7 m²/s, 0.32 to 0.57 x 10-7 m²/s, and 0.32 to 0.60 x 10-7 m²/s at 30, 40, and 50°C respectively for the moisture ranges studied. The temperature and moisture content effect were not significant (p>0.05) with specific heat and thermal diffusivity but significant (p<0.05) with thermal conductivity in third-order polynomial. A non-linear relationship was established between the three thermal properties and moisture content within the studied temperature range. The resulting regression models for the thermal properties gave a high coefficient of determinations (R2 ≥ 0.7995) which implies that they can be used to describe the relationships between temperature, moisture, and thermal properties of jack bean seeds.

PENGARUH BAHAN PENGENYAL KOMERSIAL FOOD GRADE DAN TEPUNG KORO PEDANG PRA GERMINASI TERHADAP PENINGKATAN KUALITAS TIWUL INSTAN TINGGI PROTEIN

The aim of the research was to make high protein instant tiwul from mocaf which was substituted with jack bean flour and given a food grade commercial thickener to improve its sensory aspect. The study used a randomized block design. The factors studied were germination treatment on jack bean (G), without pre germination (G1) and with pre germination (G2); Proportion of mocaf: jack bean flour : tapioca (w/w) (F), P1 = 75:15:10, P2 = 70:20:10 and P3 = 65:25:10 and Addition of Commercial food grade thickening agent (w/w; % to flour weight) (K), K1 = 0.5%, K2 = 0.75% and K3 = 1.0%. The variables studied were physicochemical properties (moisture content, ash content, formol value, rehydration ability, for the best treatment, protein and fat content were tested) and sensory properties. Data were analyzed by F test (ANOVA) and continued with DMRT (Duncan Multiple Range Test). Based on the effectiveness index test, the best instant tiwul was G2P3K3 (with pre germination; proportion of mocaf-jack bean flour-tapioca w/w 65:25:10; commercial food grade thickening agent 1%). This instant tiwul has a water content of 4.39% wb; protein 7.05%db (6.74%wb), fat 6.39% db (6.11%wb), ash 0.98%db (0.93%wb) and carbohydrates (by difference) 85.54% db (81.78% wb), coefficient rehydration 3,67 and formol value 0,0383 ml NaOH 0,1N/g bk.

Pasting properties of jack bean (Canavalia ensiformis) modified starch with heat moisture treatment

Jack bean is a local legume that can grow in suboptimal land and potential to be developed in Indonesia. Jack bean seed has 33.32% of protein and 61.15% of carbohydrate so it can become a starch source for the food industry. Native starch has a limited application on food products because of its weak characteristics such as being unstable to heat and shear. Heat Moisture Treatment (HMT) starch modification is needed to overcome the weaknesses. HMT is a natural modification because it does not leave a chemical residue. The objective of this research was to investigate the effects of heat moisture treatment (HMT) with various moisture content and time on the pasting properties (peak, through, breakdown, final, setback viscosity, peak time, and peak temperature) of jack bean modified starch. This research used Factorial Completely Randomized Design (FCRD) with two factors, varying moisture content (27%, 30%, 33%) and time (10 h, 13 h, 16 h). The results show that the various moisture leads to increase peak-time, pasting temperature and decrease peak viscosity, through viscosity, breakdown, final and setback viscosity. In addition, higher HMT time could increase peak time, pasting temperature but decrease peak, trough, breakdown, final and setback viscosity.

Soil urease inhibition by various plant extracts

Urea is the most popular and widely used nitrogenous fertilizer. High soil urease activity rapidly hydrolyses applied urea to ammonia which contributes to soil nitrogen (N) losses and reduces N use efficiency of crop plants. The ammonia losses can be minimized by the inhibition of soil urease activity which has been explored using various potential chemical inhibitors. However, the soil urease activity inhibition potential of plant extracts is rarely explored to date. In the present study, extracts of 35 plant materials were taken and evaluated against jack bean urease. Eleven extracts, showing >50% jack bean urease inhibition, were selected and further investigated in 13 soils collected from various districts of Punjab, Pakistan. Interestingly, except Capsicum annum, Melia azedarach, Citrus reticulata and Quercus infectoria, the plant extracts showed urease inhibition activities in soils, the extent of which was lower as compared to that observed in jack bean urease though. Maximum urea hydrolysis inhibition (70%) was noted with Vachellia nilotica which was 40% more than that of hydroquinone (50%) followed by that of Eucalyptus camaldulensis (24%). The extracts of V. nilotica and E. camaldulensis were coated on urea and applied to soil in the next step. At 21st day, 239% and 116% more urea-N was recovered from soil treated with V. nilotica and E. camaldulensis extracts coated urea, respectively, as compared to uncoated urea. Conclusively, these results indicated that the coating of V. nilotica and E. camaldulensis extracts on urea prills prolonged urea persistence in soil owing to minimum urea hydrolysis, probably, the extracts of V. nilotica and E. camaldulensis showed their urease inhibition potential. The results of this study provide a base line for the identification of new soil urease inhibitor compounds from plant materials in future.

The evaluation of protease enzyme effectivenes in broiler chicken diet containing jack bean seed (Canavalia ensiformis) with different protein level toward internal organ size

This study was conducted with the intention of evaluating the use of the protease enzyme in broiler feed containing jack bean seed (Canavalia ensiformis) with the different levels of protein towards the internal organs of broiler chicken starter. Bird samples used were 24 broilers of the male lohmann strain taken from 120 chickens. The study design used was a factorial completely randomized design (FCRD) with 2 factors treatment and 3 replications (each replication used 2 samples of bird). The first factor was the level of protein (CP 22% and CP 19.5%) and the second factor was the use of enzymes (non-protease and protease). Data were analyzed by using analysis of variance (ANOVA). The result showed that the use of low protein levels in broiler feed containing jack bean seed significantly (P <0.05) increased the percentage of pancreatic weights but could be improved by the use of protease enzymes. Levels of protein treatment did not significantly affect the percentage of heart, liver and bile weights. The use of protease enzymes significantly (P <0.05) decreased the percentage of pancreatic and bile weights but did not significantly affect the percentage of liver and heart weights.

Hybrid Multivalent Jack Bean α-Mannosidase Inhibitors: The First Example of Gold Nanoparticles Decorated with Deoxynojirimycin Inhitopes

Among carbohydrate-processing enzymes, Jack bean α-mannosidase (JBα-man) is the glycosidase with the best responsiveness to the multivalent presentation of iminosugar inhitopes. We report, in this work, the preparation of water dispersible gold nanoparticles simultaneously coated with the iminosugar deoxynojirimycin (DNJ) inhitope and simple monosaccharides (β-d-gluco- or α-d-mannosides). The display of DNJ at the gold surface has been modulated (i) by using an amphiphilic linker longer than the aliphatic chain used for the monosaccharides and (ii) by presenting the inhitope, not only in monomeric form, but also in a trimeric fashion through combination of a dendron approach with glyconanotechnology. The latter strategy resulted in a strong enhancement of the inhibitory activity towards JBα-man, with a Ki in the nanomolar range (Ki = 84 nM), i.e., more than three orders of magnitude higher than the monovalent reference compound.

Strategies to terminate summer cover crops for weed management in no-tillage vegetable production in southeast Brazil

Cover crop residue left on the soil surface as organic mulch in no-tillage crop production provides several environmental benefits, including weed suppression. Thus, many farmers who use cover crops attempt to reduce the use of agricultural inputs, especially herbicides. Therefore, our objectives were to study the potential of different cover crop species to suppress weeds and produce an in situ organic mulch, and evaluate the effect of the organic mulch with and without spraying glyphosate on weed suppression for vegetable (tomato (Solanum lycopersicum L. and broccoli (Brassica oleracea L. var. botrytis) growth and yield. Five cover crop treatments (sunn hemp (Crotalaria juncea L.), jack bean [Canavalia ensiformis (L.) DC.], pearl millet [Pennisetum glaucum (L.) R. Br.], grain sorghum [Sorghum bicolor (L.) Moench ssp. bicolor] and a no-cover crop (control)) were used in the main plots; and spraying or no spraying glyphosate on the flattened cover crop in the sub plots of split-plot experimental design. Organic mulch from pearl millet, sorghum and sunn hemp resulted in lower weed biomass during the early season of both tomato and broccoli than jack bean and no-cover crop (control). Spraying glyphosate after roller crimping reduced weed biomass by 103 g m−2 and 20 g m−2 by 45 and 60 days after transplanting (DAT) of tomato, respectively and resulted in a better tomato yield compared to non spraying. Glyphosate reduced weed biomass by 110 g m−2 in the early season of broccoli (30 DAT), but did not affect yield. Terminating high biomass cover crops with a roller crimper is a promising technique for weed management in vegetable crops, which has the potential to reduce or even eliminate the need for herbicide.