scholarly journals Characterization of Vitamin B12-Binding Proteins Isolated from Human Milk and Saliva by Affinity Chromatography

1974 ◽  
Vol 249 (22) ◽  
pp. 7220-7227
Author(s):  
Robert L. Burger ◽  
Robert H. Allen
Keyword(s):  
Human Milk ◽  
Vitamin B12 ◽  
Affinity Chromatography ◽  
Binding Proteins

Rabbit antibodies against the low molecular weight folate binding protein from human milk. Use for immunological characterization of human folate binding proteins in an enzyme-linked immunosorbent assay (ELISA)

1987 ◽  
Vol 7 (7) ◽  
pp. 553-557 ◽  
Author(s):  
Mimi Høier-Madsen ◽  
Steen Ingemann Hansen ◽  
Jan Holm
Keyword(s):  
Molecular Weight ◽  
Human Milk ◽  
Immunosorbent Assay ◽  
Binding Proteins ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Low Molecular Weight ◽  
Folate Binding Protein ◽  
Folate Binding Proteins

Antibodies to a low molecular weight folate binding protein isolated from human milk were raised in rabbits and used for development of a two-site enzyme-linked immunosorbent assay (ELISA) for immunological characterization of human folate binding proteins (FBPs). The high and low molecular weight FBPs from human milk were immunologically indistinguishable. Furthermore, the FBPs in human urine and cerebrospinal fluid showed a cross-reactivity of 70% and 30%, respectively. No cross-reactivity of the FBP from cow's milk was observed.

scholarly journals Functional and phylogenetic characterization of noncanonical vitamin B12–binding proteins in zebrafish suggests involvement in cobalamin transport

2018 ◽  
Vol 293 (45) ◽  
pp. 17606-17621 ◽  
Author(s):  
Courtney R. Benoit ◽  
Abigail E. Stanton ◽  
Aileen C. Tartanian ◽  
Andrew R. Motzer ◽  
David M. McGaughey ◽  
...  
Keyword(s):  
Vitamin B12 ◽  
Binding Proteins ◽  
Phylogenetic Characterization
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