scholarly journals Correction to: Asymmetric flow field-flow fractionation coupled to surface plasmon resonance detection for analysis of therapeutic proteins in blood serum

2021 ◽  
Author(s):  
Mats Leeman ◽  
Willem M. Albers ◽  
Radoslaw Bombera ◽  
Johana Kuncova-Kallio ◽  
Jussipekka Tuppurainen ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Flow Field ◽  
Blood Serum ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Therapeutic Proteins ◽  
Field Flow Fractionation ◽  
Asymmetric Flow ◽  
Flow Fractionation ◽  
Resonance Detection

scholarly journals Asymmetric flow field-flow fractionation coupled to surface plasmon resonance detection for analysis of therapeutic proteins in blood serum

2020 ◽  
Author(s):  
Mats Leeman ◽  
Willem M. Albers ◽  
Radoslaw Bombera ◽  
Johana Kuncova-Kallio ◽  
Jussipekka Tuppurainen ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Flow Field ◽  
Blood Serum ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Biological Samples ◽  
Specific Activity ◽  
Field Flow Fractionation ◽  
Asymmetric Flow ◽  
Flow Fractionation

Abstract Coupling of surface plasmon resonance (SPR) detection to asymmetric flow field-flow fractionation (AF4) offers the possibility to study active fractions of bio-separations on real samples, such as serum and saliva, including the assessment of activity of possibly aggregated species. The coupling of SPR with AF4 requires the possibility to select fractions from a fractogram and redirect them to the SPR. The combination of SPR with chromatography-like methods also requires a mechanism for regeneration of the receptor immobilised onto the SPR sensor surface. In recent work, the combination of size exclusion chromatography (SEC) with SPR was pioneered as a successful methodology for identification, characterisation and quantification of active biocomponents in biological samples. In this study, the approach using AF4 is evaluated for the antibody trastuzumab in buffer and serum. The particular object of this study was to test the feasibility of using AF4 in combination with SPR to detect and quantify proteins and aggregates in complex samples such as blood serum. Also, in the investigation, three different immobilisation methods for the receptor HER-2 were compared, which involved (1) direct binding via EDC/NHS, the standard approach; (2) immobilisation via NTA-Ni-Histag complexation; and (3) biotin/avidin-linked chemistry using a regenerable form of avidin. The highest specific activity was obtained for the biotin-avidin method, while the lowest specific activity was observed for the NTA-Ni-Histag linkage. The data show that AF4 can separate trastuzumab monomers and aggregates in blood serum and that SPR has the ability to selectively monitor the elution. This is an encouraging result for automated analysis of complex biological samples using AF4-SPR.

scholarly journals Limitations of Nanoparticles Size Characterization by Asymmetric Flow Field‑Fractionation Coupled with Online Dynamic Light Scattering

2021 ◽  
Author(s):  
Francesco Giorgi ◽  
Judith M. Curran ◽  
Douglas Gilliland ◽  
Rita La Spina ◽  
Maurice Whelan ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Flow Field ◽  
Field Flow Fractionation ◽  
Asymmetric Flow ◽  
Uv Detector ◽  
Elution Time ◽  
On Line ◽  
Real Size ◽  
Flow Fractionation

AbstractThe development of reliable protocols suitable for the characterisation of the physical properties of nanoparticles in suspension is becoming crucial to assess the potential biological as well as toxicological impact of nanoparticles. Amongst sizing techniques, asymmetric flow field flow fractionation (AF4) coupled to online size detectors represents one of the most robust and flexible options to quantify the particle size distribution in suspension. However, size measurement uncertainties have been reported for on-line dynamic light scattering (DLS) detectors when coupled to AF4 systems. In this work we investigated the influence of the initial concentration of nanoparticles in suspension on the sizing capability of the asymmetric flow field-flow fractionation technique coupled with an on-line dynamic light scattering detector and a UV–Visible spectrophotometer (UV) detector. Experiments were performed with suspensions of gold nanoparticles with a nominal diameter of 40 nm and 60 nm at a range of particle concentrations. The results obtained demonstrate that at low concentration of nanoparticles, the AF4-DLS combined technique fails to evaluate the real size of nanoparticles in suspension, detecting an apparent and progressive size increase as a function of the elution time and of the concentration of nanoparticles in suspension.

Molar Mass Characterization of Crude Lignosulfonates by Asymmetric Flow Field-Flow Fractionation

2018 ◽  
Vol 7 (1) ◽  
pp. 216-223 ◽  
Author(s):  
Irina Sulaeva ◽  
Philipp Vejdovszky ◽  
Ute Henniges ◽  
Arnulf Kai Mahler ◽  
Thomas Rosenau ◽  
...  
Keyword(s):  
Flow Field ◽  
Molar Mass ◽  
Field Flow Fractionation ◽  
Asymmetric Flow ◽  
Flow Fractionation

Surface plasmon resonance detection of small molecule using split aptamer fragments

2011 ◽  
Vol 156 (2) ◽  
pp. 893-898 ◽  
Author(s):  
Qing Wang ◽  
Jiahao Huang ◽  
Xiaohai Yang ◽  
Kemin Wang ◽  
Leiliang He ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Small Molecule ◽  
Plasmon Resonance ◽  
Resonance Detection
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