CTAB electrophoresis and immunoblotting: a new method for the determination of soy protein in meat products

1997 ◽  
Vol 205 (3) ◽  
pp. 224-226 ◽  
Author(s):  
Monika Körs ◽  
H. Steinhart
Keyword(s):  
Soy Protein ◽  
Meat Products ◽  
New Method

Improved Enzyme-Linked Immunosorbent Assay for Determination of Soy Protein in Meat Products

1987 ◽  
Vol 70 (3) ◽  
pp. 582-587
Author(s):  
James H Rittenburg ◽  
Alexandra Adams ◽  
John Palmer ◽  
John C Allen
Keyword(s):  
Sample Preparation ◽  
Immunosorbent Assay ◽  
Soy Protein ◽  
Meat Products ◽  
Enzyme Linked Immunosorbent Assay ◽  
Liquid Form ◽  
Protein Levels ◽  
Wet Weight ◽  
Meat Samples

Abstract An Indirect, Competitive Enzyme-Linked Immunosorbent Assay (Elisa) Has Been Developed For Quantitation Of Soy Protein In Meat Products. The Methodology Allows Rapid Aqueous Extraction Of Meat Samples Into A Liquid Form Suitable For Assay. The Assay Is Highly Specific For Soy Protein And Is Designed To Measure Soy Protein Levels Between 1 And 10% Of The Wet Weight Of The Sample. Standardized, Stabilized Reagents For Carrying Out The Procedure Are Commercially Available In A Kit. The Analysis, Including Sample Preparation, Can Be Completed Within A Workday, And The Actual Immunoassay In Less Than 60 Min.

Determination of Soy in Meat

1987 ◽  
Vol 70 (1) ◽  
pp. 85-90
Author(s):  
David B Berkowitz ◽  
Donald W Webert
Keyword(s):  
Soy Protein ◽  
Protein Conformation ◽  
Gel Electrophoresis ◽  
Protein Denaturation ◽  
Sodium Dodecylsulfate ◽  
Meat Products ◽  
Soy Flour ◽  
Soy Proteins ◽  
Immunological Methods

Abstract A number of methods may be used for determining soy flour in meat products. Highly purified soy products are more difficult to determine because the nonprotein components used to quantify the flour are reduced. Immunoassays have been used to directly measure protein content of soy products. Immunological methods for determination of soy proteins in meat are complicated by changes in the structure of the soy proteins during processing. These changes alter the available epitopes, changing the immunoreactivity of soy proteins. The epitopes available are dictated by the details of the processing. Other workers circumvented this problem by denaturing the soy protein with urea and mercaptoethanol, and then removing these agents by dialysis; whatever the initial protein conformation, all soy samples came to the same final conformation after the denaturing agents were removed. The assay used antibody made against the "renatured protein." These steps made the assay long and laborious. Attempts to develop a rapid assay were complicated by the same protein denaturation problems. Sodium dodecylsulfate gel electrophoresis coupled with immunoblotting may be the best quantitative approach.

Soxtec Fat Analyzer for Determination of Total Fat in Meat: Collaborative Study

1992 ◽  
Vol 75 (2) ◽  
pp. 288-292 ◽  
Author(s):  
Max L Foster ◽  
Sharon E Gonzales
Keyword(s):  
Collaborative Study ◽  
Meat Products ◽  
New Method ◽  
Pork Sausage ◽  
Beef Patties ◽  
Soxhlet Method ◽  
Repeatability And Reproducibility ◽  
Total Fat ◽  
Improved Performance

Abstract A new method for determination of total fat in meat and meat products, which uses a commercially available extraction system, was collaboratively studied by 11 laboratories. The study compared the new Soxtec solvent extraction with the Soxhlet method. The new method reduces extraction time, enables recovery of 60-70% of the solvent, and improves safety through external heating. Each laboratory received 6 samples: canned ham, ground beef, frankfurters, fresh pork sausage, hard salami, and beef patties with added soy. Laboratories were Instructed to analyze each sample In duplicate on each of 2 days by both the Soxhlet and Soxtec methods. In general, results for the Soxtec system showed Improved performance. For all samples used in Soxtec analysis, ranges of values for repeatability and reproducibility were Sr, 0.106-0.764; RSDr, 1.01-2.44%; SR, 0.112- 0.799; and RSDR, 1.53-2.84%. The method was adopted first action by AOAC International.

A new method for the determination of nitrates

1960 ◽  
Vol 23 ◽  
pp. 227-232 ◽  
Author(s):  
P WEST ◽  
G LYLES
Keyword(s):  
New Method

A New Method for the Determination of Plasma Activators of Fibrinolysis

1977 ◽  
Vol 37 (02) ◽  
pp. 210-215 ◽  
Author(s):  
R Margalit ◽  
E Gidron ◽  
Y Shalitin
Keyword(s):  
New Method ◽  
Effective Activator

SummaryThe term “effective activator” of plasminogen is proposed, to denote the resultant of activator-antiactivator interaction, and a method for the determination of the level of these activators is described. By adding axcess plasminogen to the euglobulin fraction of plasma the influence of the level of endogenous plasminogen and of the antiplasmin is eliminated. It is shown that the level of fibrinogen has very little bearing on the results. An effective activator unit is defined as equal to 1 CTA unit of urokinase activity on a fibrinogen-plasminogen substrate.

The Plasmin Inhibitors of Plasma

1964 ◽  
Vol 12 (01) ◽  
pp. 119-125 ◽  
Author(s):  
Y Shamash ◽  
A Rimon
Keyword(s):  
Human Plasma ◽  
New Method ◽  
Normal Amount ◽  
Caseinolytic Activity ◽  
Before And After ◽  
Plasmin Inhibitors

SummaryA new method for the assay of plasmin inhibitors in human plasma is described. The method consists of determination of the caseinolytic activity of a standard plasmin solution before and after incubation with the inhibitor, with lysine added to the mixture as a stabilizer of plasmin. Using this method, it was found that plasma contains enough inhibitors to inactivate 30 caseinolytic units of plasmin, or 10 times the normal amount of plasminogen in human plasma.

AGGLUTINATION INHIBITION REACTIONS FOR THE DETERMINATION OF GONADOTROPHINS

1969 ◽  
Vol 62 (1_Suppl) ◽  
pp. S95-S112 ◽  
Author(s):  
A. H. W. M. Schuurs
Keyword(s):  
Immune System ◽  
Quantitative Determination ◽  
Haemagglutination Inhibition ◽  
Latex Agglutination ◽  
Literature Survey ◽  
New Method ◽  
Test Fluid ◽  
Main Application ◽  
Test Systems

ABSTRACT Various techniques for sensitising erythrocytes and latex particles with gonadotrophins, particularly with HCG, are described. The haemagglutination inhibition reactions are generally interpreted by means of »erythrocyte settling patterns«. By a new method of evaluating these patterns a relatively precise quantitative determination is possible. Latex agglutination inhibition reactions on slides are particularly suitable as rapid qualitative tests. In cases where the maximum attainable sensitivity of the agglutination inhibition tests is insufficient, e. g. for determining LH concentrations in urine, the hormone in the test fluid has to be concentrated or extracted. An alternative method is a modified haemagglutination inhibition test for large volumes which is applicable to unconcentrated urine. Due to non-specific inhibitions the above-mentioned tests cannot be applied to unprocessed serum. Agglutination inhibition tests with HCG are already well advanced, pregnancy diagnosis being their main application. Now that highly purified HCG is available, a satisfactory specificity for these tests can be attained. If the immune system for HCG is used for estimating LH, it has to meet additional specificity requirements. Furthermore, the measure of cross-reaction and the choice of standard merit special attention. Finally, a literature survey is given of test systems in which LH and FSH were used as antigens.

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