Primary Cultures of Embryonic Chick Lens Cells as a Model System to Study Lens Gap Junctions and Fiber Cell Differentiation

2012 ◽  
Vol 245 (7) ◽  
pp. 357-368 ◽  
Author(s):  
Linda S. Musil
Keyword(s):  
Cell Differentiation ◽  
Gap Junctions ◽  
Primary Cultures ◽  
Model System ◽  
Fiber Cell ◽  
Embryonic Chick ◽  
Lens Cells ◽  
Chick Lens

scholarly journals Dye transfer between cells of the embryonic chick lens becomes less sensitive to CO2 treatment with development.

1982 ◽  
Vol 92 (3) ◽  
pp. 694-705 ◽  
Author(s):  
S M Schuetze ◽  
D A Goodenough
Keyword(s):  
Gap Junctions ◽  
Developmental Stage ◽  
Fluorescent Dyes ◽  
Morphological Changes ◽  
Freeze Fracture ◽  
Developmental State ◽  
High Pco2 ◽  
Dye Transfer ◽  
Lens Cells ◽  
Chick Lens

During the 3-h developmental stage 14 in the chick, intercellular transfer of iontophoresed fluorescent dyes becomes less sensitive to the lowering of intracellular pH by either CO2 or acetate ions. Up to developmental state 14, dye transfer between lens cells is reversibly blocked by exposure to 50% CO2. Beyond stage 14, dye transfer between these cells is no longer reversibly blocked by elevated pCO2. Electronic coupling is present throughout lens development and is not reversibly blocked by high pCO2 at any stage. The gap junctions joining the lens cells show morphological changes at developmental stage 14. Up to stage 14, all gap junctions observed between chick lens cells have connexon assemblies that appear condensed or crystalline following routine freeze-fracture microscopy. Beyond stage 14, chick lens cells express gap junctions with both the condensed assemblies and the dispersed assemblies characteristic of adult lens gap-junction structure.

Effect of X-Irradiation and Vitamin C on DNA Degradation and Endogenous DNase in Embryonic Chick Lens Cells

1987 ◽  
Vol 6 (11) ◽  
pp. 1275-1281 ◽  
Author(s):  
J. R. Trevithick ◽  
E. Chaudun ◽  
A. S. Muel ◽  
Y. Courtois ◽  
M. F. Counis
Keyword(s):  
Vitamin C ◽  
Dna Degradation ◽  
Embryonic Chick ◽  
Lens Cells ◽  
X Irradiation ◽  
Chick Lens

Lipid composition of chick lens fiber cell gap junctions

1982 ◽  
Vol 2 (9) ◽  
pp. 569-578 ◽  
Author(s):  
Jose Alcala ◽  
Malkhan Katar ◽  
Harry Maisel
Keyword(s):  
Gap Junctions ◽  
Lipid Composition ◽  
Fiber Cell ◽  
Lens Fiber ◽  
Lens Fiber Cell ◽  
Chick Lens

Contacts between pigmented retina epithelial cells in culture

1976 ◽  
Vol 22 (2) ◽  
pp. 371-383 ◽  
Author(s):  
C.A. Middleton ◽  
S.M. Pegrum
Keyword(s):  
Gap Junctions ◽  
Tight Junctions ◽  
Primary Cultures ◽  
Embryonic Chick ◽  
Isolated Cells ◽  
Gradual Reduction ◽  
Extensive Region ◽  
Cytoplasmic Filaments ◽  
Junctional Complexes ◽  
Number Of Cells

The behaviour of primary cultures of dissociated embryonic chick pigmented retina epithelial (PRE) cells has been investigated. Isolated PRE cells have a mean speed of locomotion of 7–16 mum/h. Collisions between the cells normally result in the development of stable contacts between the cells involved. This leads to a gradual reduction in the number of isolated cells and an increase in the number of cells incorporated into islands. Ultrastructural observations of islands of cells after 24 h in culture show that junctional complexes are present between the cells. These complexes consist of 2 components: (a) an apically situated region of focal tight junctions and/or gap junctions, and (b) a more ventrally located zonula adhaerens with associated cytoplasmic filaments forming a band running completely around the periphery of each cell. The intermembrane gap in the region of the zonula is 6-0-12-0 nm. The junctional complexes become more differentiated with time and after 48 h in culture consist of an extensive region of tight junctions and/or gap junctions and a more specialized zonula adhaerens. It is suggested that the development of junctional complexes may be responsible for the stable contacts that the cells display in culture.

Protein synthesis in embryonic chick lens cells

1967 ◽  
Vol 23 (3) ◽  
pp. 577-IN25 ◽  
Author(s):  
Ronald Reeder ◽  
Eugene Bell
Keyword(s):  
Protein Synthesis ◽  
Embryonic Chick ◽  
Lens Cells ◽  
Chick Lens
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