scholarly journals Polyamine Triggering of Exocytosis in Paramecium Involves an Extracellular Ca 2+ /(Polyvalent Cation)-Sensing Receptor, Subplasmalemmal Ca-Store Mobilization and Store-Operated Ca 2+ -Influx via Unspecific Cation Channels

2000 ◽  
Vol 174 (2) ◽  
pp. 141-156 ◽  
Author(s):  
N. Klauke ◽  
M.-P. Blanchard ◽  
H. Plattner
Keyword(s):  
Cation Channels ◽  
Polyvalent Cation ◽  
Cation Sensing

Localization of the extracellular Ca2+/polyvalent cation-sensing protein in rat kidney

1998 ◽  
Vol 274 (3) ◽  
pp. F611-F622 ◽  
Author(s):  
Daniela Riccardi ◽  
Amy E. Hall ◽  
Naibedya Chattopadhyay ◽  
Jason Z. Xu ◽  
Edward M. Brown ◽  
...  
Keyword(s):  
Rat Kidney ◽  
Regional Distribution ◽  
Receptor Expression ◽  
Receptor Protein ◽  
Thick Ascending Limb ◽  
Polyvalent Cation ◽  
Amino Acid Region ◽  
Oxide Synthase ◽  
Brain Nitric Oxide ◽  
Cation Sensing

We previously identified transcripts encoding a G protein-coupled, extracellular calcium/polyvalent cation-sensing receptor, RaKCaR, in rat kidney (D. Riccardi, J. Park, W.-S. Lee, G. Gamba, E. M. Brown, and S. C. Hebert. Proc. Natl. Acad. Sci. USA 92: 131–135, 1994), which was proposed to provide the mechanism for modulating a variety of renal functions in response to changes in extracellular Ca2+ (E. M. Brown. In: Handbook of Physiology. Bethesda, MD: Am. Physiol. Soc., 1992, sect. 8, vol. 2, chapt. 39, p. 1841–1916; and S. C. Hebert. Kidney Int. 50: 2129–2139, 1996). Here, we examine the cellular and regional distribution of receptor protein by immunofluorescence microscopy using a polyclonal antibody raised against a 22 amino acid region of the NH2 terminus of the receptor. The most intense fluorescence was seen at the basolateral border of cortical thick ascending limb cells. Basolateral staining for the receptor was also detected in medullary thick ascending limbs, in macula densa cells identified by costaining with antibody to brain nitric oxide synthase, NOS-B1, and in distal convoluted tubule cells distinguished by costaining for the apical thiazide-sensitive Na+-Cl−cotransporter. Apical anti-RaKCaR staining was detected at the base of the brush border of proximal tubules with decreasing intensity from S1 to S3 segments. In cortical collecting ducts, anti-RaKCaR staining was detected in some, but not all, type A intercalated cells identified by costaining with anti-H+-ATPase and anti-AE1 Cl−/[Formula: see text]exchanger antibodies. The present study demonstrates that RaKCaR protein is expressed in many different nephron segments and that the polarity of receptor expression varies with cell type along the nephron. These results suggest potential roles for the extracellular Ca2+/polyvalent cation-sensing receptor in responding to both circulating and urinary concentrations of divalent minerals and potentially other polyvalent cations (e.g., aminoglycoside antibiotics) to modulate nephron function.

scholarly journals Molecular and Functional Identification of a Ca2+(Polyvalent Cation)-sensing Receptor in Rat Pancreas

1999 ◽  
Vol 274 (29) ◽  
pp. 20561-20568 ◽  
Author(s):  
Jason I. E. Bruce ◽  
Xuesong Yang ◽  
Carole J. Ferguson ◽  
Austin C. Elliott ◽  
Martin C. Steward ◽  
...  
Keyword(s):  
Functional Identification ◽  
Polyvalent Cation ◽  
Rat Pancreas ◽  
Cation Sensing

Polyvalent Cation-Sensing Mechanism Increased Na+-Independent Mg2+ Transport in Renal Epithelial Cells

2001 ◽  
Vol 287 (3) ◽  
pp. 671-674 ◽  
Author(s):  
Akira Ikari ◽  
Kumiko Nakajima ◽  
Kazuya Kawano ◽  
Yasunobu Suketa
Keyword(s):  
Epithelial Cells ◽  
Polyvalent Cation ◽  
Renal Epithelial Cells ◽  
Sensing Mechanism ◽  
Cation Sensing

scholarly journals Activation of a polyvalent cation-sensing receptor decreases magnesium transport via claudin-16

2008 ◽  
Vol 1778 (1) ◽  
pp. 283-290 ◽  
Author(s):  
Akira Ikari ◽  
Chiaki Okude ◽  
Hayato Sawada ◽  
Yohei Sasaki ◽  
Yasuhiro Yamazaki ◽  
...  
Keyword(s):  
Polyvalent Cation ◽  
Magnesium Transport ◽  
Cation Sensing

Nickel Enhances Collagen-induced Platelet Activation Acting by Increasing the Organization of the Cytoskeleton

1998 ◽  
Vol 79 (02) ◽  
pp. 395-399 ◽  
Author(s):  
Fabio Pulcinelli ◽  
Silvia Sebastiani ◽  
Marzia Pesciotti ◽  
Pasquale Pignatelli ◽  
James Daniel ◽  
...  
Keyword(s):  
Platelet Aggregation ◽  
Platelet Adhesion ◽  
Cell Types ◽  
Cytochalasin D ◽  
Selective Activation ◽  
Polyvalent Cation ◽  
Induce Platelet Aggregation ◽  
Triton X ◽  
Adhesion Of Platelets ◽  
Cation Sensing

SummaryIn keratinocytes, osteoclasts and enterocytes, Ni2+ acts as an agonist working through selective activation of the polyvalent cation-sensing receptor.We report here that while Ni2+ alone had no direct ability to induce platelet aggregation or secretion, Ni2+ pretreatment produced these responses when platelets were stimulated with subthreshold concentrations of collagen. In addition, pretreatment with Ni2+ significantly enhanced collagen-induced phospholipase C activation and calcium mobilization. Platelet adhesion to collagen was increased and the inhibition of collagen-induced adhesion normally seen after cytochalasin D treatment was significantly diminished.When Ni2+ was added to platelets alone, tyrosine phosphorylation of p60src was increased. Moreover, Ni2+ enhanced the amount of protein, especially actin, found in the low-speed Triton X-100 insoluble cyto-skeleton. Our results indicate that nickel, possibly acting via a platelet cation sensing receptor analogous to that which has been described in other cell types, may cause a rapid tyrosine kinase-dependent cyto-skeleton reorganization leading to enhanced adhesion of platelets to collagen and increasing collagen-dependent responses.

scholarly journals Cloning and functional expression of a rat kidney extracellular calcium/polyvalent cation-sensing receptor.

1995 ◽  
Vol 92 (1) ◽  
pp. 131-135 ◽  
Author(s):  
D. Riccardi ◽  
J. Park ◽  
W. S. Lee ◽  
G. Gamba ◽  
E. M. Brown ◽  
...  
Keyword(s):  
Rat Kidney ◽  
Functional Expression ◽  
Extracellular Calcium ◽  
Polyvalent Cation ◽  
Cation Sensing

scholarly journals Mg2+/Ca2+sensing inhibits hormone-stimulated Mg2+ uptake in mouse distal convoluted tubule cells

1998 ◽  
Vol 275 (3) ◽  
pp. F353-F360 ◽  
Author(s):  
Brian W. Bapty ◽  
Long-Jun Dai ◽  
Gordon Ritchie ◽  
Lucie Canaff ◽  
Geoffrey N. Hendy ◽  
...  
Keyword(s):  
Distal Convoluted Tubule ◽  
Dependent Manner ◽  
Cellular Mechanisms ◽  
Polyvalent Cation ◽  
Concentration Dependent Manner ◽  
Nephron Segment ◽  
The Mean ◽  
Free Media ◽  
Cation Sensing ◽  
Camp Formation

The distal convoluted tubule plays a significant role in renal magnesium conservation. An immortalized mouse distal convoluted tubule (MDCT) cell line has been extensively used to study the cellular mechanisms of magnesium transport in this nephron segment. MDCT cells possess an extracellular polyvalent cation-sensing mechanism responsive to Mg2+, Ca2+, and neomycin. The present studies determined the effect of Mg2+/Ca2+sensing on hormone-mediated cAMP formation and Mg2+ uptake in MDCT cells. MDCT cells were Mg2+ depleted by culturing in Mg2+-free media for 16 h, and Mg2+ uptake was measured by microfluorescence after placing the depleted cells in 1.5 mM MgCl2. The mean rate of Mg2+ uptake was 164 ± 5 nM/s in control MDCT cells. Activation of Mg2+/Ca2+sensing with neomycin did not affect basal Mg2+ uptake (155 ± 5 nM/s). We have previously reported that treatment of MDCT cells with either glucagon or arginine vasopressin (AVP) stimulated Mg2+ entry. In the present studies, the addition of extracellular Mg2+ or Ca2+ inhibited glucagon- and AVP-stimulated cAMP formation and Mg2+ uptake in concentration-dependent manner with half-maximal concentrations of ∼1.5 and 3.0 mM, respectively. Exogenous cAMP or forskolin stimulated Mg2+ uptake in the presence of Mg2+/Ca2+sensing activation. We infer from these studies that Mg2+/Ca2+-sensing mechanisms located in the distal convoluted tubule may play a role in control of distal magnesium absorption.

Cation channels of the TRPC family contribute to development of nephropathy and retinopathy in the STZ model

2015 ◽  
Vol 10 (S 01) ◽  
Author(s):  
M Freichel ◽  
D Schumacher ◽  
C Matka ◽  
I Mathar ◽  
U Kriebs ◽  
...  
Keyword(s):  
Cation Channels
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