Expression of foreign proteins in Escherichia coli by fusing with an archaeal FK506 binding protein

2004 ◽  
Vol 64 (1) ◽  
pp. 99-105 ◽  
Author(s):  
A. Ideno ◽  
M. Furutani ◽  
T. Iwabuchi ◽  
T. Iida ◽  
Y. Iba ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Binding Protein ◽  
Fk506 Binding Protein ◽  
Foreign Proteins

scholarly journals FK506 Binding Protein from the Hyperthermophilic Archaeon Pyrococcus horikoshii Suppresses the Aggregation of Proteins in Escherichia coli

2002 ◽  
Vol 68 (2) ◽  
pp. 464-469 ◽  
Author(s):  
Akira Ideno ◽  
Masahiro Furutani ◽  
Yoshitaka Iba ◽  
Yoshikazu Kurosawa ◽  
Tadashi Maruyama
Keyword(s):  
Escherichia Coli ◽  
Protein Folding ◽  
Binding Protein ◽  
Fab Fragment ◽  
Ppiase Activity ◽  
Pyrococcus Horikoshii ◽  
E Coli ◽  
Fk506 Binding Protein ◽  
Soluble Recombinant Protein

ABSTRACT The 29-kDa FK506 binding protein (FKBP) gene is the only peptidyl-prolyl cis-trans isomerase (PPIase) gene in the genome of Pyrococcus horikoshii. We characterized the function of this FKBP (PhFKBP29) and used it to increase the production yield of soluble recombinant protein in Escherichia coli. The PPIase activity (k cat/Km ) of PhFKBP29 was found to be much lower than that of other archaeal 16- to 18-kDa FKBPs by a chymotrypsin-coupled assay of the oligo-peptidyl substrate at 15�C. Besides this low PPIase activity, PhFKBP29 showed chaperone-like protein folding activity which enhanced the refolding yield of chemically unfolded rhodanese in vitro. In addition, it suppressed thermal protein aggregation in a temperature range of 45 to 100�C. When the PhFKBP29 gene was coexpressed with the recombinant Fab fragment gene of the anti-hen egg lysozyme antibody in the cytoplasm of E. coli, whose expressed product tended to form an inactive aggregate in E. coli, it improved the yield of the soluble Fab fragments with antibody specificity. PhFKBP29 exerted protein folding and aggregation suppression in E. coli cells.

An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein

Gene ◽  
1988 ◽  
Vol 74 (2) ◽  
pp. 365-373 ◽  
Author(s):  
Claude V. Maina ◽  
Paul D. Riggs ◽  
Andres G. Grandea ◽  
Barton E. Slatko ◽  
Laurie S. Moran ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Binding Protein ◽  
Maltose Binding Protein ◽  
Foreign Proteins

Autoantibodies to FK506 Binding Protein 12 (FKBP12) in Autoimmune Diseases

Autoimmunity ◽  
1999 ◽  
Vol 29 (3) ◽  
pp. 159-170 ◽  
Author(s):  
Nobuhiko Shinkura ◽  
Iwao Ikai ◽  
Akira Yamauchl ◽  
Tetsuro Hirose ◽  
Yasuhiro Kawap ◽  
...  
Keyword(s):  
Autoimmune Diseases ◽  
Binding Protein ◽  
Fk506 Binding Protein
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