scholarly journals Characterization of the family GH54 α-l-arabinofuranosidases in Penicillium funiculosum, including a novel protein bearing a cellulose-binding domain

2010 ◽  
Vol 87 (3) ◽  
pp. 1007-1021 ◽  
Author(s):  
Olivier Guais ◽  
Olivier Tourrasse ◽  
Marion Dourdoigne ◽  
Jean Luc Parrou ◽  
Jean Marie Francois
Keyword(s):  
Binding Domain ◽  
Penicillium Funiculosum ◽  
Cellulose Binding Domain ◽  
The Family ◽  
Cellulose Binding ◽  
Novel Protein

The cellulose-binding domain (CBDCex) of an exoglucanase fromCellulomonas fimi: Production inEscherichia coli and characterization of the polypeptide

1993 ◽  
Vol 42 (4) ◽  
pp. 401-409 ◽  
Author(s):  
Edgar Ong ◽  
Neil R. Gilkes ◽  
Robert C. Miller ◽  
R. Antony J. Warren ◽  
Douglas G. Kilburn
Keyword(s):  
Binding Domain ◽  
Inescherichia Coli ◽  
Cellulose Binding Domain ◽  
Cellulose Binding

scholarly journals Characterization of the cellulose-binding domain of the Clostridium cellulovorans cellulose-binding protein A.

1993 ◽  
Vol 175 (18) ◽  
pp. 5762-5768 ◽  
Author(s):  
M A Goldstein ◽  
M Takagi ◽  
S Hashida ◽  
O Shoseyov ◽  
R H Doi ◽  
...  
Keyword(s):  
Binding Protein ◽  
Protein A ◽  
Binding Domain ◽  
Cellulose Binding Domain ◽  
Clostridium Cellulovorans ◽  
Cellulose Binding

Purification of a Fusion Protein Using the Family VI Cellulose-Binding Domain of Clostridium stercorarium XynAa

1998 ◽  
Vol 864 (1 ENZYME ENGINE) ◽  
pp. 485-488 ◽  
Author(s):  
KAZUO SAKKA ◽  
SHUICHI KARITA ◽  
TETSUYA KIMURA ◽  
KUNIO OHMIYA
Keyword(s):  
Fusion Protein ◽  
Binding Domain ◽  
Cellulose Binding Domain ◽  
The Family ◽  
Cellulose Binding

Characterization of aNeocallimastixpatriciarumxylanase gene and its product

1999 ◽  
Vol 45 (11) ◽  
pp. 970-974 ◽  
Author(s):  
Jin-Hao Liu ◽  
Brent L Selinger ◽  
Cheng-Fang Tsai ◽  
Kuo-Jaon Cheng
Keyword(s):  
Catalytic Domain ◽  
Xylanase Activity ◽  
Binding Domain ◽  
Glycosyl Hydrolases ◽  
Cellulose Binding Domain ◽  
Xylanase Gene ◽  
Neocallimastix Patriciarum ◽  
Cellulose Binding ◽  
Linker Domain

A xylanase gene (xynC) isolated from the anaerobic ruminal fungus Neocallimastix patriciarum was characterized. The gene consists of an N-terminal catalytic domain that exhibited homology to family 11 of glycosyl hydrolases, a C-terminal cellulose binding domain (CBD) and a putative dockerin domain in between. Each domain was linked by a short linker domain rich in proline and alanine. Deletion analysis demonstrated that the CBD was essential for optimal xylanase activity of the enzyme, while the putative dockerin domain may not be required for enzyme function.Key words: xylanase, cellulose binding domain, Neocallimastix patriciarum.

scholarly journals Site-Directed Mutagenesis and Expression of the Soluble Form of the Family IIIa Cellulose Binding Domain from the Cellulosomal Scaffolding Protein of Clostridium cellulovorans

2005 ◽  
Vol 187 (20) ◽  
pp. 7146-7149 ◽  
Author(s):  
Koichiro Murashima ◽  
Akihiko Kosugi ◽  
Roy H. Doi
Keyword(s):  
Binding Domain ◽  
Site Directed Mutagenesis ◽  
Scaffolding Protein ◽  
Soluble Form ◽  
Directed Mutagenesis ◽  
Cellulose Binding Domain ◽  
Binding Studies ◽  
Clostridium Cellulovorans ◽  
The Family ◽  
Cellulose Binding

ABSTRACT The planar and anchoring residues of the family IIIa cellulose binding domain (CBD) from the cellulosomal scaffolding protein of Clostridium cellulovorans were investigated by site-directed mutagenesis and cellulose binding studies. By fusion with maltose binding protein, the family IIIa recombinant wild-type and mutant CBDs from C. cellulovorans were expressed as soluble forms. Cellulose binding tests of the mutant CBDs indicated that the planar strip residues played a major role in cellulose binding and that the anchoring residues played only a minor role.

scholarly journals Humicola grisea var. thermoidea cbh1.2: A new gene in the family of cellobiohydrolases is expressed and encodes a cellulose-binding domain-less enzyme.

1997 ◽  
Vol 43 (2) ◽  
pp. 115-120 ◽  
Author(s):  
Marcio J. Poças-Fonseca ◽  
Beatriz D. Lima ◽  
Marcelo M. Brígido ◽  
Ildinete Silva-Pereira ◽  
Maria Sueli S. Felipe ◽  
...  
Keyword(s):  
Binding Domain ◽  
Cellulose Binding Domain ◽  
The Family ◽  
New Gene ◽  
Humicola Grisea ◽  
Cellulose Binding
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