Yeast genes involved in sulfur and nitrogen metabolism affect the production of volatile thiols from Sauvignon Blanc musts

2012 ◽  
Vol 97 (1) ◽  
pp. 223-235 ◽  
Author(s):  
Michael J. Harsch ◽  
Richard C. Gardner
OENO One ◽  
2015 ◽  
Vol 49 (4) ◽  
pp. 253 ◽  
Author(s):  
Pierre Helwi ◽  
Cécile Thibon ◽  
Aude Habran ◽  
Ghislaine Hilbert ◽  
Sabine Guillaumie ◽  
...  

<p style="text-align: justify;"><strong>Aim</strong>: To determine the effect of vine nitrogen status in interaction with grapevine variety and rootstock on vine development and on the synthesis of 3-sulfanylhexan-1-ol precursors (Glut-3SH and Cys-3SH) in grape berries produced in controlled conditions.</p><p style="text-align: justify;"><strong>Methods and results</strong>: Potted Sauvignon blanc (SB) and Cabernet-Sauvignon (CS) vines, grafted on two different rootstocks (110R and RGM), were irrigated with two nutritive solutions varying only by their nitrogen content (N(-) and N(+)). Vine nitrogen status of N(-) treatment was significantly different from that of N(+) treatment. Secondary leaf area was higher in N(+) treatment and ripening was delayed because of increased vigor. High N status increased Glut-3SH content in berries, while it did not impact Cys-3SH level. Moreover, the concentrations of 3SH precursors were higher in SB berries compared to CS and their synthesis was enhanced in berries produced by vines grafted onto RGM under N(+) treatment.</p><p style="text-align: justify;"><strong>Conclusion</strong>: Glut-3SH content was mainly determined by plant N status. Grapevine variety and rootstock/N treatment interaction also had a significant, although more limited, impact. Cys-3SH level was dependent on berry developmental stage and grapevine variety, but not on vine N status.</p><strong>Significance and impact of the study</strong>: A better understanding of the effect of terroir components on the biosynthesis of the precursors of volatile thiols is gained.


OENO One ◽  
2006 ◽  
Vol 40 (1) ◽  
pp. 1 ◽  
Author(s):  
Xavier Choné ◽  
Valérie Lavigne-Cruège ◽  
Takatoshi Tominaga ◽  
Cornelis Van Leeuwen ◽  
Caroline Castagnède ◽  
...  

<p style="text-align: justify;">Vine nitrogen status influence on S-cysteine conjugate precursors of volatile thiols, glutathione and phenolic content of the berries of Vitis vinifera L. cv. Sauvignon blanc have been assessed. Despite an increase in berry weight, the increase in nitrogen supply in the vine leads to higher cysteine precursor levels in grape juice. We also show that a late addition of nitrogen at berry set leads to a lower level of phenolic compounds in white grapes and to higher glutathione levels. Therefore, in white varieties, and particularly Sauvignon blanc, improving the nitrogen supply of the vine clearly increases its aromatic potential. However, an excess of nitrogen supply would increase the grape sensitivity to Botrytis cinerea and therefore decrease its quality.</p>


OENO One ◽  
2008 ◽  
Vol 42 (3) ◽  
pp. 125 ◽  
Author(s):  
Florian Lacroux ◽  
Olivier Trégoat ◽  
Cornelius Van Leeuwen ◽  
Alexandre Pons ◽  
Takatoshi Tominaga ◽  
...  

<p style="text-align: justify;"><strong>Aims</strong>: It is well known that vine nitrogen deficiency can negatively impact on aroma in white wines. Soil nitrogen fertilization enhances aroma expression, but it also increases vine vigour and susceptibility to grey rot. The aim of this work was to investigate the impact of foliar nitrogen as well as foliar nitrogen and sulphur applications on aromatic expression, vigour and susceptibility to grey rot of Vitis vinifera L. cv. Sauvignon blanc.</p><p style="text-align: justify;"><strong>Methods and results</strong>: The impact of foliar nitrogen (N) and sulphur (S) application on aromatic expression of Vitis vinifera L. cv. Sauvignon blanc has been investigated. On a plot where vine nitrogen status is naturally low and water availability not limiting, foliar nitrogen and sulphur fertilization (10 kg/ha of N and 5 kg/ha of S) increased yeast available nitrogen content. Vine vigour and maturity level of grapes were not modified compared to the control. The wines produced from N+S vines contained more volatile thiols and glutathione. These results were confirmed by a tasting of wines produced with grapes from the experimental plots. Foliar nitrogen fertilization alone (10 kg/ha of N) also increased Sauvignon blanc aromatic expression, but less so than the N + S treatment.</p><p style="text-align: justify;"><strong>Conclusions</strong>: Foliar N and foliar N + S applications can enhance aromatic expression in Vitis vinifera L. cv. Sauvignon blanc wines without increasing vine vigour and infestation by grey rot.</p><p style="text-align: justify;"><strong>Significance and impact of the study</strong>: Vine nitrogen deficiency can negatively impact on grape aroma potential. Soil nitrogen application can increase vine nitrogen status, but it has several drawbacks: it increases vigour and enhances Botrytis susceptibility. This study shows that foliar N and foliar N + S applications can improve vine nitrogen status and enhance aroma expression in Sauvignon blanc wines without the negative impact on vigour and Botrytis susceptibility. Although this study was carried out on Sauvignon blanc vines, it is likely that foliar N or foliar N + S applications will have similar effects on other grapevine varieties containing volatile thiols (Colombard, Riesling, Petit Manseng and Sémillon).</p>


OENO One ◽  
2002 ◽  
Vol 36 (4) ◽  
pp. 205 ◽  
Author(s):  
Isabelle Masneuf-Pomarède ◽  
Marie-Laure Murat ◽  
Gennadi I. Naumov ◽  
Takatoshi Tominaga ◽  
Denis Dubourdieu

<p style="text-align: justify;">We measured ability of some indigenous <em>Saccharomyces bayanus</em> var. <em>uvarum</em> wine yeasts to release volatile thiols from their S-cysteine conjugate precursors, odorous compounds responsible for the characteristic aroma of Sauvignon blanc wines. We also made interspecific hybrids between <em>Saccharomyces cerevisiae</em> and <em>Saccharomyces bayanus</em> var. <em>uvarum</em> strains and verified their hybrid origin with karyotypes and <em>MET2</em> PCR-RFLP analysis. As compared to the parents, some hybrids could release high amounts of volatile thiols from the S-cysteine conjugate precursor without producing excessive amounts of β-phenylethyl alcohol and its acetate. One hybrid was retained for industrial production under a dry form and successfully compared with <em>Saccharomyces cerevisiae</em> strains in experimental tests in different cellars.</p>


Author(s):  
Takatoshi Tominaga ◽  
Anton Furrer ◽  
Robert Henry ◽  
Denis Dubourdieu

1960 ◽  
Vol 19 (1) ◽  
pp. 44-53 ◽  
Author(s):  
G. A. McLaren ◽  
G. C. Anderson ◽  
J. A. Welch ◽  
C. D. Campbell ◽  
G. S. Smith
Keyword(s):  

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