Increased riboflavin production by manipulation of inosine 5′-monophosphate dehydrogenase in Ashbya gossypii

2015 ◽  
Vol 99 (22) ◽  
pp. 9577-9589 ◽  
Author(s):  
Rubén M. Buey ◽  
Rodrigo Ledesma-Amaro ◽  
Mónica Balsera ◽  
José María de Pereda ◽  
José Luis Revuelta
Keyword(s):  
Ashbya Gossypii ◽  
Riboflavin Production ◽  
Monophosphate Dehydrogenase

Effects of sirtuins on the riboflavin production in Ashbya gossypii

2021 ◽  
Author(s):  
Tatsuya Kato ◽  
Junya Azegami ◽  
Mai Kano ◽  
Hesham A. El Enshasy ◽  
Enoch Y. Park
Keyword(s):  
Ashbya Gossypii ◽  
Riboflavin Production

Isolation of Ashbya gossypii mutant for an improved riboflavin production targeting for biorefinery technology

2007 ◽  
Vol 103 (2) ◽  
pp. 468-476 ◽  
Author(s):  
E.Y. Park ◽  
J.H. Zhang ◽  
S. Tajima ◽  
L. Dwiarti
Keyword(s):  
Ashbya Gossypii ◽  
Riboflavin Production

scholarly journals Isolation and characterization of an Ashbya gossypii mutant for improved riboflavin production

2012 ◽  
Vol 43 (2) ◽  
pp. 441-448 ◽  
Author(s):  
Shiping Wei ◽  
James Hurley ◽  
Zhenglong Jiang ◽  
Siwen Wang ◽  
Yuanyuan Wang
Keyword(s):  
Ashbya Gossypii ◽  
Riboflavin Production ◽  
Isolation And Characterization

scholarly journals The improvement of riboflavin production in Ashbya gossypii via disparity mutagenesis and DNA microarray analysis

2011 ◽  
Vol 91 (5) ◽  
pp. 1315-1326 ◽  
Author(s):  
Enoch Y. Park ◽  
Yoko Ito ◽  
Masashi Nariyama ◽  
Takashi Sugimoto ◽  
Dwiarti Lies ◽  
...  
Keyword(s):  
Microarray Analysis ◽  
Dna Microarray ◽  
Ashbya Gossypii ◽  
Riboflavin Production ◽  
Dna Microarray Analysis

Improved riboflavin production with Ashbya gossypii from vegetable oil based on 13C metabolic network analysis with combined labeling analysis by GC/MS, LC/MS, 1D, and 2D NMR

2018 ◽  
Vol 47 ◽  
pp. 357-373 ◽  
Author(s):  
Susanne Katharina Schwechheimer ◽  
Judith Becker ◽  
Lindsay Peyriga ◽  
Jean-Charles Portais ◽  
Daniel Sauer ◽  
...  
Keyword(s):  
Network Analysis ◽  
Metabolic Network ◽  
Vegetable Oil ◽  
2D Nmr ◽  
Ashbya Gossypii ◽  
Riboflavin Production ◽  
Metabolic Network Analysis

Yap1-dependent oxidative stress response provides a link to riboflavin production in Ashbya gossypii

2012 ◽  
Vol 49 (9) ◽  
pp. 697-707 ◽  
Author(s):  
Andrea Walther ◽  
Jürgen Wendland
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Oxidative Stress Response ◽  
Ashbya Gossypii ◽  
Riboflavin Production

scholarly journals Genomic analysis of a riboflavin-overproducing Ashbya gossypii mutant isolated by disparity mutagenesis

2019 ◽  
Author(s):  
Tatsuya Kato ◽  
Junya Azegami ◽  
Ami Yokomori ◽  
Hideo Dohra ◽  
Hesham A. El Enshasy ◽  
...  
Keyword(s):  
Genomic Analysis ◽  
Tca Cycle ◽  
Nucleotide Metabolism ◽  
Repair System ◽  
Ashbya Gossypii ◽  
Riboflavin Production ◽  
Dna Mismatch ◽  
Pyrimidine Nucleotide ◽  
Dna Mismatch Repair System ◽  
Genes Encoding

Abstract Background: Ashbya gossypii naturally overproduces riboflavin and has been utilized for industrial riboflavin production. To improve riboflavin production, various approaches have been developed. In this study, to investigate the change in metabolism of a riboflavin-overproducing mutant, namely, the W122032 strain (MT strain) that was isolated by disparity mutagenesis, genomic analysis was carried out. Results: In the genomic analysis, 33 homozygous and 1377 heterozygous mutations were detected. Among these heterozygous mutations, the proportion of mutated reads in each gene was different, ranging from 21 to 75%. These results suggest that the MT strain exhibits a mixture of diploidy, triploidy and tetraploidy, or contains multiple nuclei containing different mutations. We tried to isolate haploid spores from the MT strain to prove it ploidy, but these spores were never isolated. This result indicate MT strain does not produce its spores. Homozygous and heterozygous mutations were found in genes encoding enzymes involved in amino acid metabolism, the TCA cycle, purine and pyrimidine nucleotide metabolism and the DNA mismatch repair system. Especially, genes encoding enzymes in mitochondria had many homozygous and heterozygous mutations intensively. These results suggest that the overproduction of riboflavin in the MT strain may be associated with mitochondrial dysfunction. Conclusion: This study provides new insights into riboflavin production in A. gossypii and the usefulness of disparity mutagenesis for the creation of new types of mutants for metabolic engineering.

Riboflavin production by Ashbya gossypii

2011 ◽  
Vol 34 (4) ◽  
pp. 611-618 ◽  
Author(s):  
Tatsuya Kato ◽  
Enoch Y. Park
Keyword(s):  
Ashbya Gossypii ◽  
Riboflavin Production
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