Metabolic engineering of Saccharomyces cerevisiae using the CRISPR/Cas9 system to minimize ethyl carbamate accumulation during Chinese rice wine fermentation

2020 ◽  
Vol 104 (10) ◽  
pp. 4435-4444 ◽  
Author(s):  
Dianhui Wu ◽  
Wenjuan Xie ◽  
Xiaomin Li ◽  
Guolin Cai ◽  
Jian Lu ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Engineering ◽  
Ethyl Carbamate ◽  
Wine Fermentation ◽  
Rice Wine ◽  
Chinese Rice Wine

scholarly journals Chinese Yellow Rice Wine Processing with Reduced Ethyl Carbamate Formation by Deleting Transcriptional Regulator Dal80p in Saccharomyces cerevisiae

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3580
Author(s):  
Tianyu Wei ◽  
Zhihua Jiao ◽  
Jingjin Hu ◽  
Hanghang Lou ◽  
Qihe Chen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethyl Carbamate ◽  
Wine Fermentation ◽  
Yeast Cells ◽  
Transcriptional Level ◽  
Rice Wine ◽  
Chinese Rice Wine ◽  
Positive Regulators ◽  
External Sources ◽  
Urea Metabolism

Ethyl carbamate (EC) is a potential carcinogen that forms spontaneously during Chinese rice wine fermentation. The primary precursor for EC formation is urea, which originates from both external sources and arginine degradation. Urea degradation is suppressed by nitrogen catabolite repression (NCR) in Saccharomyces cerevisiae. The regulation of NCR is mediated by two positive regulators (Gln3p, Gat1p/Nil1p) and two negative regulators (Dal80p/Uga43p, Deh1p/Nil2p/GZF3p). DAL80 revealed higher transcriptional level when yeast cells were cultivated under nitrogen-limited conditions. In this study, when DAL80-deleted yeast cells were compared to wild-type BY4741 cells, less urea was accumulated, and genes involved in urea utilization were up-regulated. Furthermore, Chinese rice wine fermentation was conducted using dal80Δ cells; the concentrations of urea and EC were both reduced when compared to the BY4741 and traditional fermentation starter. The findings of this work indicated Dal80p is involved in EC formation possibly through regulating urea metabolism and may be used as the potential target for EC reduction.

scholarly journals Cloning and Purification of Ornithine Transcarbamoylase from Lactobacillus brevis and Its Application on Regulating Ethyl Carbamate Formation in Chinese rice wine

2020 ◽  
Vol 5 (5) ◽  
pp. 218-225
Author(s):  
Anthony Putera Setiawan ◽  
Dionne Debra Mazvita ◽  
Huang Wendi ◽  
Jin Chengyu
Keyword(s):  
Lactobacillus Brevis ◽  
Ethyl Carbamate ◽  
Arginine Deiminase ◽  
Wine Fermentation ◽  
Flavor Compounds ◽  
Rice Wine ◽  
Chinese Rice Wine ◽  
Volatile Flavor Compounds ◽  
Volatile Flavor ◽  
Ornithine Transcarbamoylase

It was studied that ornithine transcarbamoylase played important roles in ethyl carbamate (EC) formation. Ornithine transcarbamoylase (OTCase) can degrade citrulline, a precursor of EC, through arginine deiminase pathway. Therefore, they are generally added to regulate EC catabolism in rice wine fermentation. In this work, OTCase were added in different concentration and ferment time. It turned out addition of OTCase could reduce EC to some extent, especially for low concentration enzyme addition and mid-stage addition time. Furthermore, the production of amino acids, volatile flavor compounds and sense of taste were not markedly affected. The discoveries reveal that EC can be reduced by supplying OTCase while rice wine leavening. Key words: Ethyl carbamate, Ornithine transcarbamoylase, Rice wine fermentation, Enzyme inhibition

scholarly journals Effect of citrulline metabolism inSaccharomyces cerevisiaeon the formation of ethyl carbamate during Chinese rice wine fermentation

2018 ◽  
Vol 124 (1) ◽  
pp. 77-84 ◽  
Author(s):  
Dianhui Wu ◽  
Xiaomin Li ◽  
Junyong Sun ◽  
Guolin Cai ◽  
Guangfa Xie ◽  
...  
Keyword(s):  
Ethyl Carbamate ◽  
Wine Fermentation ◽  
Rice Wine ◽  
Chinese Rice Wine

scholarly journals Genomic Evolution of Saccharomyces cerevisiae under Chinese Rice Wine Fermentation

2014 ◽  
Vol 6 (9) ◽  
pp. 2516-2526 ◽  
Author(s):  
Yudong Li ◽  
Weiping Zhang ◽  
Daoqiong Zheng ◽  
Zhan Zhou ◽  
Wenwen Yu ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Wine Fermentation ◽  
Rice Wine ◽  
Genomic Evolution ◽  
Chinese Rice Wine
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