Thermostable ?-amylase production by immobilized Bacillus licheniformis cells in agar gel and on acrylonitrile/acrylamide membranes

1994 ◽  
Vol 41 (5) ◽  
pp. 517-522 ◽  
Author(s):  
A. Tonkova ◽  
V. Ivanova ◽  
E. Dobreva ◽  
M. Stefanova ◽  
D. Spasova
Keyword(s):  
Bacillus Licheniformis ◽  
Agar Gel ◽  
Amylase Production ◽  
Thermostable Amylase

Synthesis of Thermostable Amylase Enzyme from Bacillus licheniformis and it's Optimization to Different Sources

2013 ◽  
Vol 760 ◽  
pp. 73-78
Author(s):  
Anima Nanda ◽  
T. Sudhakar ◽  
B.K. Nayak ◽  
J. Prem Kumar
Keyword(s):  
Bacillus Licheniformis ◽  
Enzyme Production ◽  
Organic Nitrogen ◽  
Sodium Dodecyl ◽  
Nitrogen Sources ◽  
Enzyme Productivity ◽  
Amylase Production ◽  
Organic Nitrogen Source ◽  
Different Sources ◽  
Thermostable Amylase

S: - Among the six isolated amylase producing strains,Bacillus licheniformis(B1), the thermostable strain was selected from the soil of a paddy field. Its enzyme productivity and activity were evaluated. The activity of enzyme was calculated as 27.77 IU/ml. Effects of various carbon and organic nitrogen sources, and C/N ratio on enzyme production were examined. Maximum α - amylase production was obtained in medium containing 1% starch. Fructose supported the maximum amylase production among all the sugar studied. Of the organic nitrogen sources tested, peptone was found to be the best organic nitrogen source for excess yield of the enzyme. The optimum C/N ratio was found to be 1:1. The α amylase exhibited activity at a wide pH and temperature range and activity were found to be optimal at pH 6 and 40 °C respectively. The molecular weight of α amylase was calculated by sodium dodecyl sulphate gel electrophoresis and found to be around 29,000 Daltons.

Thermostable α-amylase production by immobilized Bacillus licheniformis cells in agar gel and on acrylonitrile/acrylamide membranes

1994 ◽  
Vol 41 (5) ◽  
pp. 517-522 ◽  
Author(s):  
A. Tonkova ◽  
V. Ivanova ◽  
E. Dobreva ◽  
M. Stefanova ◽  
D. Spasova
Keyword(s):  
Bacillus Licheniformis ◽  
Agar Gel ◽  
Amylase Production

Thermostable 1-amylase production by Bacillus licheniformis cells immobilized on polyacrylates with cyclic carbonate groups in the side chain

1998 ◽  
Vol 153 (2) ◽  
pp. 157-162 ◽  
Author(s):  
E. Dobreva ◽  
V. Ivanova ◽  
M. Stefanova ◽  
A. Tonkova ◽  
L. Kabaivanova ◽  
...  
Keyword(s):  
Bacillus Licheniformis ◽  
Cyclic Carbonate ◽  
Side Chain ◽  
Amylase Production

Pearl millet, a source of alpha amylase production by Bacillus licheniformis

2005 ◽  
Vol 96 (10) ◽  
pp. 1201-1204 ◽  
Author(s):  
Ikram-ul-Haq ◽  
Hamad Ashraf ◽  
Qadeer M.A. ◽  
Javed Iqbal
Keyword(s):  
Pearl Millet ◽  
Bacillus Licheniformis ◽  
Alpha Amylase ◽  
Amylase Production

scholarly journals Production of thermostable extracellular α-amylase by a moderate thermophilic Bacillus licheniformis isolated from Qinarje Hot Spring (Ardebil prov. of Iran)

2017 ◽  
Vol 118 (4) ◽  
Author(s):  
Ali Deljou ◽  
Iman Arezi
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Bacillus Licheniformis ◽  
Bacterial Growth ◽  
Enzyme Production ◽  
Hot Spring ◽  
Basal Medium ◽  
Thermophilic Bacterium ◽  
Inoculum Size ◽  
Amylase Production

Background and Purpose: Amylases are most important industrial enzymes that account for about 30% of the world’s food, feed, fermentation, textile, detergent and cellulosic industries. This study aimed at optimum production of thermostable α-amylase via moderate thermophilic bacterium (Bacillus licheniformis) which was recently isolated from Qinarje Hot spring.Material and Methods: Initially, ability of bacterium for amylase activity was determined by starch hydrolysis test using Gram’s iodine staining. Then bacterial growth pattern and amylase production curves in basal production medium were graphically determined at different time intervals. Finally, effect of different temperature, pH, carbon source, nitrogen source, minerals and inoculum size were studied on bacterial growth and amylase production using turbidimetric and DNS method, respectively.Results: Optimum enzyme production achieved after 84 hours of inoculation from cultures growing at 40 ˚C and pH 9.0 in a medium containing 0.03% (w/v) of CaCl2, compared to the basal medium, results showed that the best enzyme production happened with inoculum size of 4% (v/v). The addition of 1% (w/v) rice husk (as a Carbon source) enhanced enzyme productivity up to 160% and substitution of the peptone and yeast extract with 1% (w/v) of tryptone (as a Nitrogen source) increased the α-amylase production up to 160%.Conclusion: Our findings show that B. licheniformis-AZ2 strain has an ability to produce the thermostable α-amylase which is suitable in starch processing and food industries. To be commercialized, further investigation is required for enhancement of the enzyme production.Keywords: Bacillus licheniformis; Optimization; Basal medium; Agricultural by-products.

scholarly journals The use of sorghum for thermostable amylase production from Thermoactinomyces thalpophilus

1997 ◽  
Vol 25 (1) ◽  
pp. 13-16 ◽  
Author(s):  
G.C. Uguru ◽  
J.A. Akinyanju ◽  
A. Sani
Keyword(s):  
Amylase Production ◽  
Thermostable Amylase
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