scholarly journals Production of thermostable extracellular α-amylase by a moderate thermophilic Bacillus licheniformis isolated from Qinarje Hot Spring (Ardebil prov. of Iran)

2017 ◽  
Vol 118 (4) ◽  
Author(s):  
Ali Deljou ◽  
Iman Arezi
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Bacillus Licheniformis ◽  
Bacterial Growth ◽  
Enzyme Production ◽  
Hot Spring ◽  
Basal Medium ◽  
Thermophilic Bacterium ◽  
Inoculum Size ◽  
Amylase Production

Background and Purpose: Amylases are most important industrial enzymes that account for about 30% of the world’s food, feed, fermentation, textile, detergent and cellulosic industries. This study aimed at optimum production of thermostable α-amylase via moderate thermophilic bacterium (Bacillus licheniformis) which was recently isolated from Qinarje Hot spring.Material and Methods: Initially, ability of bacterium for amylase activity was determined by starch hydrolysis test using Gram’s iodine staining. Then bacterial growth pattern and amylase production curves in basal production medium were graphically determined at different time intervals. Finally, effect of different temperature, pH, carbon source, nitrogen source, minerals and inoculum size were studied on bacterial growth and amylase production using turbidimetric and DNS method, respectively.Results: Optimum enzyme production achieved after 84 hours of inoculation from cultures growing at 40 ˚C and pH 9.0 in a medium containing 0.03% (w/v) of CaCl2, compared to the basal medium, results showed that the best enzyme production happened with inoculum size of 4% (v/v). The addition of 1% (w/v) rice husk (as a Carbon source) enhanced enzyme productivity up to 160% and substitution of the peptone and yeast extract with 1% (w/v) of tryptone (as a Nitrogen source) increased the α-amylase production up to 160%.Conclusion: Our findings show that B. licheniformis-AZ2 strain has an ability to produce the thermostable α-amylase which is suitable in starch processing and food industries. To be commercialized, further investigation is required for enhancement of the enzyme production.Keywords: Bacillus licheniformis; Optimization; Basal medium; Agricultural by-products.

Stimulation of dextranase production by oxidized dextran

1970 ◽  
Vol 16 (9) ◽  
pp. 841-844 ◽  
Author(s):  
Robert G. Brown
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Enzyme Production ◽  
Inorganic Nitrogen ◽  
Penicillium Funiculosum ◽  
Inorganic Nitrogen Source ◽  
Oxidized Dextran ◽  
Excess Glucose ◽  
Stimulation Of

Penicillium funiculosum, Penicillium lilacinum, and Spicaria violacea produced excellent yields of dextranase if ketodextran replaced dextran as a carbon source. Ketodextrans I and II having degrees of substitution of 2 and 20% respectively were used in this study. P. funiculosum grew equally well on dextran and ketodextran I but less well on ketodextran II. Addition of a readily metabolizable carbohydrate such as glucose, sucrose, or galactose stimulated growth on ketodextran II, resulting in better dextranase production. However, excess glucose reversed this increase in enzyme production. Replacement of an inorganic nitrogen source with an organic one further stimulated dextranase production during growth of P. funiculosum on ketodextran II.

Synthesis of Thermostable Amylase Enzyme from Bacillus licheniformis and it's Optimization to Different Sources

2013 ◽  
Vol 760 ◽  
pp. 73-78
Author(s):  
Anima Nanda ◽  
T. Sudhakar ◽  
B.K. Nayak ◽  
J. Prem Kumar
Keyword(s):  
Bacillus Licheniformis ◽  
Enzyme Production ◽  
Organic Nitrogen ◽  
Sodium Dodecyl ◽  
Nitrogen Sources ◽  
Enzyme Productivity ◽  
Amylase Production ◽  
Organic Nitrogen Source ◽  
Different Sources ◽  
Thermostable Amylase

S: - Among the six isolated amylase producing strains,Bacillus licheniformis(B1), the thermostable strain was selected from the soil of a paddy field. Its enzyme productivity and activity were evaluated. The activity of enzyme was calculated as 27.77 IU/ml. Effects of various carbon and organic nitrogen sources, and C/N ratio on enzyme production were examined. Maximum α - amylase production was obtained in medium containing 1% starch. Fructose supported the maximum amylase production among all the sugar studied. Of the organic nitrogen sources tested, peptone was found to be the best organic nitrogen source for excess yield of the enzyme. The optimum C/N ratio was found to be 1:1. The α amylase exhibited activity at a wide pH and temperature range and activity were found to be optimal at pH 6 and 40 °C respectively. The molecular weight of α amylase was calculated by sodium dodecyl sulphate gel electrophoresis and found to be around 29,000 Daltons.

Research on microbial lipid production from potato starch wastewater as culture medium by Lipomyces starkeyi

2013 ◽  
Vol 67 (8) ◽  
pp. 1802-1808 ◽  
Author(s):  
Jun-Xian Liu ◽  
Qin-Yan Yue ◽  
Bao-Yu Gao ◽  
Yan Wang ◽  
Qian Li ◽  
...  
Keyword(s):  
Cell Growth ◽  
Carbon Source ◽  
Nitrogen Source ◽  
Culture Medium ◽  
Potato Starch ◽  
Inoculum Size ◽  
Microbial Lipid ◽  
Lipomyces Starkeyi ◽  
Starch Wastewater ◽  
Source Concentration

In this paper, potato starch wastewater as culture medium was treated by the oleaginous yeast Lipomyces starkeyi to biosynthesize microbial lipid. The result indicated that carbon source types, carbon source concentration, nitrogen source types, nitrogen source concentration, inoculum size, and cultivation time all had a significant effect on cell growth and microbial lipid accumulation in batch cultures. A measure of 120 g/L of glucose concentration, 3.0 g/L of (NH4)2SO4 concentration, 10% inoculum size, and incubation time 96 h cultivated in a shaking flask at 30 °C were found to be the optimal conditions not only for cell growth but also for lipid synthesis. Under this condition, the cellular biomass and lipid content could reach 2.59 g/L and 8.88%, respectively. This work provides a new method for effective utilization of potato starch wastewater, which has particular social and economic benefits for yeast treatment technology.

scholarly journals Screening of Culture Conditions for Production of Xylanase from Landfill Soil Bacteria

2019 ◽  
Vol 19 (2) ◽  
pp. 470 ◽  
Author(s):  
Siti Nor Amira Rosli ◽  
Rohaida Che Man ◽  
Nasratun Masngut
Keyword(s):  
Carbon Source ◽  
Moisture Content ◽  
Nitrogen Source ◽  
Incubation Period ◽  
Xylanase Activity ◽  
Nitrogen Sources ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Xylanase Production ◽  
Initial Ph

Culture conditions including initial pH media, incubation period, inoculum size, type of carbon source, type of nitrogen source and its concentration, which affect xylanase production were screened via the one-factor-at-a-time approach. The bacteria used in the production of xylanase was isolated from the landfill site at Sg. Ikan, Kuala Terengganu, Malaysia. Three characterizations of the landfill soil were investigated for their moisture content, ash content, and pH. The culture conditions range used in the experimental work were between 6–30 h for the incubation period, with initial pH between 5–9, inoculum size between 1–20% v/v, carbon, nitrogen sources, and nitrogen source concentration between 1–5% w/v. Xylanase activity was estimated using dinitrosalicylic acid (DNS) based on the release of xylose under standard assay conditions. The landfill soil was observed to have pH between pH 3.4–7.2 with a moisture content between 12.4–33.7% and ash ranged between 3.5–4.3%. Results showed that the highest xylanase activity within studied ranges was recorded at 25.91±0.0641 U/mL with 10% (v/v) inoculum size, 1% (w/v) xylose as sole carbon source, mixture of 1% (w/v) peptone and 0.25% (w/v) ammonium sulphate as nitrogen sources, which was carried out at initial pH of 8.0 for 24 h incubation.

scholarly journals Isolation and Characterisation of Thermophilic Bacillus licheniformis SUNGC2 as Producer of α-Amylase from Malaysian Hot Spring

2020 ◽  
Vol 28 (S2) ◽  
Author(s):  
Marwan Jawad Msarah ◽  
Ayesha Firdose ◽  
Izyanti Ibrahim ◽  
Wan Syaidatul Aqma
Keyword(s):  
Bacillus Licheniformis ◽  
Specific Activity ◽  
Hot Spring ◽  
Ammonium Phosphate ◽  
Fold Increase ◽  
Relative Activity ◽  
Biochemical Properties ◽  
Culture Conditions ◽  
Thermophilic Microorganisms ◽  
Amylase Production

Screening of new source of novel and industrially useful enzymes is a key research pursuit in enzyme biotechnology. The study aims to report the characteristics of novel thermophilic microorganisms isolated from Sungai Klah (SK) Hot Spring, Perak, Malaysia, that can produce α-amylase. The morphological and biochemical properties were examined for SUNGC2 sample. The isolate was further screened for amylase, followed by 16S rRNA and analytical profile index (API) test. This isolate was further subjected to pH optimisation for α-amylase production. It was found that SUNGC2 was an α-amylase producer and was identified as Bacillus licheniformis SUNGC2 with NCBI accession numbers MH062901. The enzyme was found to exhibit an optimum temperature of 50°C and a pH of 7.0. The relative activity of the enzyme was obtained based on the improvement of the culture conditions. The highest amount of amylase production was 24.65 U/mL at pH 7.0, consecutively the growth was also highest at pH 7.0 with a 9.45-fold increase in specific activity by ammonium phosphate precipitation of 80% (w/v). The results showed that the bacteria isolated from the hot spring are a significant source of thermophilic enzymes that are highly promising in biotechnology.

scholarly journals Optimization of conditions for extracellular amylase production from Listeria denitrificans

2013 ◽  
pp. 73-81
Author(s):  
MN Mahmud ◽  
MN Anwar
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Incubation Period ◽  
Yeast Extract ◽  
Medium Ph ◽  
Amylase Production ◽  
Extracellular Amylase

The foodborne Listeria denitrificans was isolated from degraded rice and studied as an amylase producer. This microorganism showed maximum amylase production at temperature 27 ºC and medium pH 7.0, and on 3rd day during incubation period. Maximum amylase production by the isolate was found in medium containing 3.0 % starch as carbon source and 0.50 % yeast extract as nitrogen source. DOI: http://dx.doi.org/10.3329/cujbs.v4i1.13388 The Chittagong Univ. J. B. Sci.,Vol. 4(1&2):73-81, 2009

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