Quantitative Detection and Differentiation of Free-Living Amoeba Species Using SYBR Green–Based Real-Time PCR Melting Curve Analysis

2006 ◽  
Vol 53 (6) ◽  
pp. 506-509 ◽  
Author(s):  
Jonas Behets ◽  
Priscilla Declerck ◽  
Yasmine Delaedt ◽  
Lieve Verelst ◽  
Frans Ollevier
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Curve Analysis ◽  
Sybr Green ◽  
Quantitative Detection ◽  
Melting Curve Analysis ◽  
Free Living ◽  
Free Living Amoeba

A novel duplex SYBR Green real-time PCR with melting curve analysis method for beef adulteration detection

2021 ◽  
Vol 338 ◽  
pp. 127932
Author(s):  
Jiapeng Li ◽  
Yixuan Wei ◽  
Jinchun Li ◽  
Ruixi Liu ◽  
Suigen Xu ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Curve Analysis ◽  
Sybr Green ◽  
Melting Curve Analysis ◽  
Analysis Method

scholarly journals High-throughput avian molecular sexing by SYBR green-based real-time PCR combined with melting curve analysis

2008 ◽  
Vol 8 (1) ◽  
pp. 12 ◽  
Author(s):  
Hsueh-Wei Chang ◽  
Chun-An Cheng ◽  
De-Leung Gu ◽  
Chia-Che Chang ◽  
San-Hua Su ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Curve Analysis ◽  
Sybr Green ◽  
Melting Curve Analysis ◽  
Molecular Sexing

Molecular detection of Mycoplasma haemomuris subspecies using dnaK-targeted real-time PCR with SYBR Green I and melting curve analysis

2016 ◽  
Vol 30 (5) ◽  
pp. 291-293
Author(s):  
Hiroshi Satoh ◽  
Daiki Imai ◽  
Shigeru Sato ◽  
Kazuhisa Furuhama ◽  
Ryô Harasawa
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Molecular Detection ◽  
Melting Curve ◽  
Curve Analysis ◽  
Sybr Green ◽  
Sybr Green I ◽  
Melting Curve Analysis

Rapid identification of clinically relevant Nocardia species using real-time PCR with SYBR Green and melting-curve analysis

2006 ◽  
Vol 55 (12) ◽  
pp. 1711-1715 ◽  
Author(s):  
Mubarak Alfaresi ◽  
Abida Elkosh
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Rapid Identification ◽  
Curve Analysis ◽  
Sybr Green ◽  
Cross Reaction ◽  
Melting Curve Analysis ◽  
Bacterial Strains ◽  
Nocardia Species

The objective of this study was to develop and evaluate a rapid new method of identifying clinically relevant Nocardia species. DNA extracted from different Nocardia strains was used in a real-time PCR assay with SYBR Green and melting-curve analysis to identify Nocardia species. Ten control strains and four bacterial strains of closely related genera were employed, and samples from 28 patients were used. All Nocardia strains were identified correctly, and there was no cross-reaction with strains from genera closely related to Nocardia. The sensitivity and specificity of the method were 90 and 100 %, respectively. This method can be used to rapidly detect Nocardia species in culture, without cross-reaction with other closely related genera.

Duplex Real-time PCR assay and SYBR green I melting curve analysis for molecular identification of HPV genotypes 16, 18, 31, 35, 51 and 66

2015 ◽  
Vol 29 (1) ◽  
pp. 13-18 ◽  
Author(s):  
D. Tsakogiannis ◽  
M. Papacharalampous ◽  
E. Toska ◽  
Z. Kyriakopoulou ◽  
T.G. Dimitriou ◽  
...  
Keyword(s):  
Real Time ◽  
Molecular Identification ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Curve Analysis ◽  
Sybr Green ◽  
Sybr Green I ◽  
Melting Curve Analysis ◽  
Pcr Assay ◽  
Hpv Genotypes

scholarly journals Genotyping of Entamoeba histolytica by Real-Time Polymerase Chain Reaction with Sybr Green I and Melting Curve Analysis

1970 ◽  
Vol 4 (1) ◽  
pp. 53-60
Author(s):  
SMM Rahman ◽  
R Haque ◽  
S Roy ◽  
MMH Mondal
Keyword(s):  
Real Time ◽  
Melting Temperature ◽  
Entamoeba Histolytica ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Curve Analysis ◽  
Sybr Green ◽  
Sybr Green I ◽  
Melting Curve Analysis ◽  
Intestinal Amoebiasis

In this study, for the detection of distinct genotype of E. histolytica of human, a nested Real-Time PCR amplification of SREHP gene using SYBR Green I and melting curve analysis was done. A total of 60 specimens (stool and liver aspirate specimens), which were found Entamoeba histolytica positive by E. histolytica specific ELISA and ssrRNA gene PCR, were selected and the experiment was conducted during the period of July 2003 to June 2004. After melting curve analysis of amplified PCR products from these isolates of stool and liver aspirate specimens, 5 genotypes were found belonging to the melting temperatures 84°C, 83°C, 82°C, 81°C and 79°C. All these 5 genotypes were present in intestinal amoebiasis patients and when the genotypes from intestinal amoebiasis patients were compared with the genotypes of amoebic liver amoebiasis patients, the genotype 84°C melting temperature was found to be absent in amoebic liver amoebiasis patients. For both the cases of intestinal and amoebic liver amoebiasis patients the genotype belonging to 83°C melting temperature was more prevalent than the other genotypes which suggest that this genotype is more responsible for the development of amoebiasis. In comparison to conventional PCR method where we found 23 different banding patterns, the Real-Time PCR and melting curve analysis method was found to be more reliable for the detection of distinct genotypes of E. histolytica because with this method we found only 5 genotypes. In conclusion, this Real-Time PCR using SYBR Green I and melting curve analysis for the genotyping of E. histolytica, excludes the need of post PCR manipulations and would be helpful for the rapid detection and screening of E. histolytica genotypes among endemic population and also for the epidemiological study. Key words: Entamoeba histolytica, genotype, diarrhoea, Real-Time PCR, melting curve analysis doi:10.3329/bjvm.v4i1.1526 Bangl. J. Vet. Med. (2006). 4 (1): 53-60

Sign in / Sign up

Export Citation Format

Share Document