Simultaneous Amplification of Two Bacterial Genes: More Reliable Method of Helicobacter pylori Detection in Microbial Rich Dental Plaque Samples

2010 ◽  
Vol 62 (1) ◽  
pp. 78-83 ◽  
Author(s):  
Saima Chaudhry ◽  
Muhammad Idrees ◽  
Mateen Izhar ◽  
Arshad Kamal Butt ◽  
Ayyaz Ali Khan
Keyword(s):  
Helicobacter Pylori ◽  
Dental Plaque ◽  
Reliable Method ◽  
Bacterial Genes

scholarly journals Helicobacter pylori colonization and oral health in children

2017 ◽  
Vol 12 (2) ◽  
pp. 41-44
Author(s):  
Vasile Valeriu LUPU ◽  
◽  
Gabriela PĂDURARU ◽  
Anca ADAM ◽  
Ana-Maria DĂBULEANU ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Oral Health ◽  
Gastric Mucosa ◽  
Oral Cavity ◽  
Gold Standard ◽  
Dental Plaque ◽  
Negative Bacterium ◽  
Gram Negative ◽  
Standard Triple Therapy ◽  
H Pylori

Helicobacter pylori (H. pylori) is a microaerophilic gram-negative bacterium infecting approximately one half of the world’s population. The oral cavity and dental plaque may be a reservoir for H. pylori infection. Diagnosis of H. pylori infection in children differs from that of adults. Although H. pylori has long been known to be detected in the oral cavity, the significance of such findings are controversial. Oral H. pylori may play an important role in re-infection of the gastric mucosa. The gold standard for eradicating H. pylori infection is standard triple therapy. The studies have shown promising results in the management of both oral and gastric H. pylori.

scholarly journals Prevalence ofHelicobacter pylori vacAGenotypes andcagAGene in Dental Plaque of Asymptomatic Mexican Children

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Alejandra Mendoza-Cantú ◽  
Víctor Hugo Urrutia-Baca ◽  
Cynthia Sofía Urbina-Ríos ◽  
Myriam Angélica De la Garza-Ramos ◽  
Martha Elena García-Martínez ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Oral Cavity ◽  
Dental Plaque ◽  
Gastrointestinal Disease ◽  
Oral Infection ◽  
Rrna Gene ◽  
Mexican Children ◽  
Asymptomatic Children ◽  
Dyspeptic Symptoms ◽  
H Pylori

The variability inHelicobacter pylori vacAandcagAgenes has been related to the progression of the gastrointestinal disease; also the presence ofH. pyloriin the oral cavity has been associated with periodontal disease in adults, but, in children without dyspeptic symptoms, little is known about this. We evaluated the prevalence ofH. pyloriand the presence ofvacA/cagAgenotypes in the oral cavity of Mexican children without dyspeptic symptoms. The gingival status was measured, and dental plaque samples (n=100) were taken. 38% of children were positive forH. pylori16S rRNA gene by qPCR. A significant association betweenH. pylorioral infection and gingival status was observed (P<0.001). In 34.6% (9/26) of mild gingivitis cases,s1m2genotype was found, whiles1m1was typed in 50% (3/6) of moderate gingivitis. ThecagAprevalence amongH. pylori-positive children was 80.8% (21/26), 83.3% (5/6), and 16.7% (1/6) of cases of mild gingivitis, moderate gingivitis, and nongingivitis, respectively (P<0.001). Thes1m1/cagA+ combinational genotype was the most detected in children with gingivitis. Our results suggest that the prevalence ofH. pyloriand detection ofvacA/cagAgenotypes-associated gastrointestinal disease in the oral cavity could be related to the progression of gingivitis in asymptomatic children.

Randomized clinical trial of Helicobacter pylori from dental plaque

2001 ◽  
Vol 88 (2) ◽  
pp. 206-206 ◽  
Author(s):  
A. K. Butt ◽  
A. A. Khan ◽  
B. A. Suleman ◽  
R. Bedi
Keyword(s):  
Clinical Trial ◽  
Helicobacter Pylori ◽  
Randomized Clinical Trial ◽  
Dental Plaque

scholarly journals Dental plaque of children as probable Helicobacter pylori reservoir

2014 ◽  
Vol 8 (35) ◽  
pp. 3220-3229 ◽  
Author(s):  
F. Gonzalez-Salazar ◽  
J.G. Gerardo-Aviles ◽  
Garza J. Castro ◽  
J.N. Garza-Gonzalez ◽  
M.E. Hernandez-Garcia ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Dental Plaque

Detection of cytotoxin genotypes of Helicobacter pylori in stomach, saliva and dental plaque

2009 ◽  
Vol 54 (7) ◽  
pp. 684-688 ◽  
Author(s):  
Denise G. Silva ◽  
Roy H. Stevens ◽  
Jacyara M.B. Macedo ◽  
Rodolpho M. Albano ◽  
Marcio E.V. Falabella ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Dental Plaque

The influence of Helicobacter pylori eradication therapy on the presence of H. pylori antigens in dental plaque and saliva

2016 ◽  
Vol 6 (1) ◽  
pp. 19-24
Author(s):  
DB. Namiot ◽  
K. Leszczyńska ◽  
A. Namiot ◽  
A. Kemona ◽  
R. Bucki ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Oral Cavity ◽  
Dental Plaque ◽  
Eradication Therapy ◽  
Oral Infection ◽  
Helicobacter Pylori Eradication ◽  
Immunological Method ◽  
H Pylori ◽  
Helicobacter Pylori Eradication Therapy

Purpose: The aim of this study was to evaluate the presence of H. pylori antigens in the oral cavity (dental plaque and saliva) of patients undergoing systemic eradication therapy. Materials and methods: The study was conducted in 49 subjects with H. pylori stomach infection. H. pylori antigens in dental plaque and saliva were evaluated with immunological method. Results: In subjects with initial H. pylori oral infection, the presence of H. pylori antigens in the oral cavity 6 weeks after successful or unsuccessful H. pylori eradication therapy in the stomach was 47.0% and 50.0%, respectively. In subjects without initial oral infection with H. pylori, the presence of H. pylori antigens in the oral cavity 6 weeks after successful and unsuccessful eradication therapy in the stomach was 30.0% and 20.0%, respectively. Conclusions: The immunological method detecting H. pylori antigens in the dental plaque and saliva cannot be recommended to evaluate the efficacy of H. pylori eradication in the oral cavity.

Brush Cytology: A Reliable Method to Detect Helicobacter pylori

1996 ◽  
Vol 22 (4) ◽  
pp. 317-321 ◽  
Author(s):  
Mauro Dalla Libera ◽  
Paolo Pazzi ◽  
Giuliano Carli ◽  
Edgardo Contato ◽  
Isotta Piva ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Reliable Method ◽  
Brush Cytology

The frequency of Helicobacter pylori in dental plaque is possibly underestimated

2015 ◽  
Vol 60 (5) ◽  
pp. 782-788 ◽  
Author(s):  
Negin Amiri ◽  
Ramin Abiri ◽  
Masoumeh Eyvazi ◽  
Mohammad Reza Zolfaghari ◽  
Amirhooshang Alvandi
Keyword(s):  
Helicobacter Pylori ◽  
Dental Plaque

Domestic flies, not dental plaque, may act as reservoirs for Helicobacter pylori infection and reinfection

1998 ◽  
Vol 114 ◽  
pp. A137
Author(s):  
AC Gough ◽  
MM Ozmen ◽  
JN Primrose ◽  
CD Johnson
Keyword(s):  
Helicobacter Pylori ◽  
Dental Plaque ◽  
Helicobacter Pylori Infection

scholarly journals Detection of Helicobacter pylori in dental plaque by reverse transcription-polymerase chain reaction.

1993 ◽  
Vol 31 (4) ◽  
pp. 783-787 ◽  
Author(s):  
A M Nguyen ◽  
L Engstrand ◽  
R M Genta ◽  
D Y Graham ◽  
F A el-Zaatari
Keyword(s):  
Polymerase Chain Reaction ◽  
Helicobacter Pylori ◽  
Reverse Transcription ◽  
Dental Plaque ◽  
Chain Reaction ◽  
Polymerase Chain
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