Efficient Expression of Human Lysozyme Through the Increased Gene Dosage and Co-expression of Transcription Factor Hac1p in Pichia pastoris

2020 ◽  
Vol 77 (5) ◽  
pp. 846-854
Author(s):  
Jun Liu ◽  
Qin Han ◽  
Qikun Cheng ◽  
Yuanyuan Chen ◽  
Ruxin Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Pichia Pastoris ◽  
Gene Dosage ◽  
Human Lysozyme ◽  
Efficient Expression

scholarly journals Novel molecular biological tools for the efficient expression of fungal lytic polysaccharide monooxygenases in Pichia pastoris

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Lukas Rieder ◽  
Katharina Ebner ◽  
Anton Glieder ◽  
Morten Sørlie
Keyword(s):  
Pichia Pastoris ◽  
Biomass Conversion ◽  
Single Step ◽  
Additional Advantage ◽  
Lytic Polysaccharide Monooxygenases ◽  
Expression Host ◽  
Shake Flask Cultivation ◽  
Polysaccharide Monooxygenases ◽  
Efficient Expression ◽  
High Yields

Abstract Background Lytic polysaccharide monooxygenases (LPMOs) are attracting large attention due their ability to degrade recalcitrant polysaccharides in biomass conversion and to perform powerful redox chemistry. Results We have established a universal Pichia pastoris platform for the expression of fungal LPMOs using state-of-the-art recombination cloning and modern molecular biological tools to achieve high yields from shake-flask cultivation and simple tag-less single-step purification. Yields are very favorable with up to 42 mg per liter medium for four different LPMOs spanning three different families. Moreover, we report for the first time of a yeast-originating signal peptide from the dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit 1 (OST1) form S. cerevisiae efficiently secreting and successfully processes the N-terminus of LPMOs yielding in fully functional enzymes. Conclusion The work demonstrates that the industrially most relevant expression host P. pastoris can be used to express fungal LPMOs from different families in high yields and inherent purity. The presented protocols are standardized and require little equipment with an additional advantage with short cultivation periods.

Enhanced human lysozyme production by Pichia pastoris via periodic glycerol and dissolved oxygen concentrations control

2021 ◽  
Vol 105 (3) ◽  
pp. 1041-1050
Author(s):  
Luqiang Jia ◽  
Teng Li ◽  
Yixuan Wu ◽  
Chunsen Wu ◽  
Huaxiang Li ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Dissolved Oxygen ◽  
Human Lysozyme ◽  
Oxygen Concentrations

Efficient expression and isolation of recombinant human interleukin-11 (rhIL-11) in Pichia pastoris

2018 ◽  
Vol 146 ◽  
pp. 69-77 ◽  
Author(s):  
Kuo-Ming Yu ◽  
Johnson Yiu-Nam Lau ◽  
Manson Fok ◽  
Yuk-Keung Yeung ◽  
Siu-Ping Fok ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Efficient Expression ◽  
Interleukin 11

scholarly journals Simple and efficient expression of Agaricus meleagris pyranose dehydrogenase in Pichia pastoris

2011 ◽  
Vol 94 (3) ◽  
pp. 695-704 ◽  
Author(s):  
Christoph Sygmund ◽  
Alexander Gutmann ◽  
Iris Krondorfer ◽  
Magdalena Kujawa ◽  
Anton Glieder ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Pyranose Dehydrogenase ◽  
Efficient Expression

scholarly journals Effect of extra N-terminal residues on the stability and folding of human lysozyme expressed in Pichia pastoris

2000 ◽  
Vol 13 (4) ◽  
pp. 299-307 ◽  
Author(s):  
Shuichiro Goda ◽  
Kazufumi Takano ◽  
Yuriko Yamagata ◽  
Yoshio Katakura ◽  
Katsuhide Yutani
Keyword(s):  
Pichia Pastoris ◽  
Human Lysozyme ◽  
The Stability

scholarly journals Transcription factor Mxr1 promotes the expression of Aox1 by repressing glycerol transporter 1 in Pichia pastoris

2017 ◽  
Vol 17 (4) ◽  
Author(s):  
Chunjun Zhan ◽  
Yankun Yang ◽  
Zhenyang Zhang ◽  
Xiang Li ◽  
Xiuxia Liu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Pichia Pastoris
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