The Crystal Proteins from Bacillus thuringiensis subsp. thompsoni Display a Synergistic Activity Against the Codling Moth, Cydia pomonella

2000 ◽  
Vol 40 (3) ◽  
pp. 200-204 ◽  
Author(s):  
Cécile Rang ◽  
Lawrence A. Lacey ◽  
Roger Frutos
Keyword(s):  
Bacillus Thuringiensis ◽  
Cydia Pomonella ◽  
Codling Moth ◽  
Synergistic Activity ◽  
Crystal Proteins ◽  
Bacillus Thuringiensis Subsp

scholarly journals Biochemical, immunological and toxicological characteristics of the crystal proteins of Bacillus thuringiensis subsp. medellin

1996 ◽  
Vol 91 (2) ◽  
pp. 231-237 ◽  
Author(s):  
Sergio Orduz ◽  
Thais Diaz ◽  
Nora Restrepo ◽  
Maria M Patiño ◽  
Martha C Tamayo
Keyword(s):  
Bacillus Thuringiensis ◽  
Crystal Proteins ◽  
Bacillus Thuringiensis Subsp

scholarly journals Solubilization, Activation, and Insecticidal Activity of Bacillus thuringiensis Serovar thompsoni HD542 Crystal Proteins

2008 ◽  
Vol 74 (23) ◽  
pp. 7145-7151 ◽  
Author(s):  
Samir Naimov ◽  
Rumyana Boncheva ◽  
Rumyana Karlova ◽  
Stefan Dukiandjiev ◽  
Ivan Minkov ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Pieris Rapae ◽  
Insect Pest ◽  
Polyacrylamide Gel Electrophoresis ◽  
Cydia Pomonella ◽  
Sodium Dodecyl ◽  
Crystal Proteins ◽  
Stable Product ◽  
Insect Gut

ABSTRACT Cry15Aa protein, produced by Bacillus thuringiensis serovar thompsoni HD542 in a crystal together with a 40-kDa accompanying protein, is one of a small group of nontypical, less well-studied members of the Cry family of insecticidal proteins and may provide an alternative for the more commonly used Cry proteins in insect pest management. In this paper, we describe the characterization of the Cry15Aa and 40-kDa protein's biochemical and insecticidal properties and the mode of action. Both proteins were solubilized above pH 10 in vitro. Incubation of solubilized crystal proteins with trypsin or insect midgut extracts rapidly processed the 40-kDa protein to fragments too small to be detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, whereas the Cry15 protein yielded a stable product of approximately 30 kDa. Protein N-terminal sequencing showed that Cry15 processing occurs exclusively at the C-terminal end. Cry15 protein showed in vitro hemolytic activity, which was greatly enhanced by preincubation with trypsin or insect gut extract. Larvae of the lepidopteran insects Manduca sexta, Cydia pomonella, and Pieris rapae were susceptible to crystals, and presolubilization of the crystals enhanced activity to P. rapae. Activity for all three species was enhanced by preincubation with trypsin. Larvae of Helicoverpa armigera and Spodoptera exigua were relatively insensitive to crystals, and activity against these insects was not enhanced by prior solubilization or trypsin treatment. The 40-kDa crystal protein showed no activity in the insects tested, nor did its addition or coexpression in Escherichia coli increase the activity of Cry15 in insecticidal and hemolytic assays.

scholarly journals Effects of gamma radiation and Bacillus thuringiensis on F1 progeny of Cydia pomonella

2021 ◽  
Vol 14 (2) ◽  
pp. 99-107
Author(s):  
I. Idris ◽  
K. Hussian
Keyword(s):  
Bacillus Thuringiensis ◽  
Gamma Radiation ◽  
Cydia Pomonella ◽  
Codling Moth ◽  
Management Program ◽  
Fruit Crops ◽  
Egg Hatchability ◽  
F1 Progeny ◽  
Two Factors ◽  
Inherited Sterility

Summary The codling moth [Cydia pomonella L. (Lepidoptera: Tortricidae)] is the main pest in most apple orchards in Syria. It causes billions of dollars in loss of fruit crops every year. The present work examined the effects of gamma radiation and Bacillus thuringiensis (BT) on F1 progeny of C. pomonella. The experimental design was based on two factors, namely F1 offspring produced by males irradiated at a dose of 150 Gy, and artificial diet of BT-treated larvae. The first offspring of unirradiated and irradiated C. pomonella males, F1, were from parents treated with a commercial formulation of Bacillus thuringiensis BT (Dipel® 2X). F1 progeny of unirradiated was significantly less susceptible to BT than that of irradiated parents. The results showed high mortality in F1 progeny of C. pomonella when gamma radiation and BT were applied together. The LC50 in F1 progeny was 2.5 ppm for irradiated parents, while it was 13 ppm for unirradiated ones. A significant reduction in the fecundity and egg hatchability of F1 progeny of irradiated parents compared to unirradiated ones was observed. This study demonstrated that for F1 progeny the combination between Inherited Sterility Technique and BT can be useful to achieve an integrated pest management program of codling moth.

scholarly journals Insecticidal Activity ofBacillus thuringiensisStrains Isolated from Soil and Water

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Edyta Konecka ◽  
Jakub Baranek ◽  
Anita Hrycak ◽  
Adam Kaznowski
Keyword(s):  
Bacillus Thuringiensis ◽  
Water Sample ◽  
Insecticidal Activity ◽  
Plant Protection ◽  
Cydia Pomonella ◽  
Codling Moth ◽  
Gene Identification ◽  
Potential Utility ◽  
Pcr Method ◽  
Soil And Water

We attempted to search novelBacillus thuringiensisstrains that produce crystals with potential utility in plant protection and with higher activity than strains already used in biopesticide production. SevenB. thuringiensissoil and water isolates were used in the research. We predicted the toxicity of their crystals bycrygene identification employing PCR method. The isolate MPU B63 with interesting, according to us, genes content was used in evaluating its crystal toxicity againstCydia pomonellacaterpillars. The strain MPU B63 was cultured from water sample and hadcry1Ab,cry1B, andcry15genes. The LC50crystals of MPU B63 were compared to LC50of commercial bioinsecticide Foray determined againstC. pomonella(codling moth). The activity of MPU B63 inclusions against codling moth larvae was approximately 24-fold higher than that of Foray. The results are a promising introduction for further study evaluating the potential usefulness of isolate MPU B63 crystals in plant protection.

Activity of Bacillus thuringiensis δ-endotoxins against codling moth (Cydia pomonella L.) larvae

2006 ◽  
Vol 92 (2) ◽  
pp. 96-99 ◽  
Author(s):  
Rumyana Boncheva ◽  
Stefan Dukiandjiev ◽  
Ivan Minkov ◽  
Ruud A. de Maagd ◽  
Samir Naimov
Keyword(s):  
Bacillus Thuringiensis ◽  
Cydia Pomonella ◽  
Codling Moth
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