scholarly journals Insecticidal Activity ofBacillus thuringiensisStrains Isolated from Soil and Water

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Edyta Konecka ◽  
Jakub Baranek ◽  
Anita Hrycak ◽  
Adam Kaznowski

We attempted to search novelBacillus thuringiensisstrains that produce crystals with potential utility in plant protection and with higher activity than strains already used in biopesticide production. SevenB. thuringiensissoil and water isolates were used in the research. We predicted the toxicity of their crystals bycrygene identification employing PCR method. The isolate MPU B63 with interesting, according to us, genes content was used in evaluating its crystal toxicity againstCydia pomonellacaterpillars. The strain MPU B63 was cultured from water sample and hadcry1Ab,cry1B, andcry15genes. The LC50crystals of MPU B63 were compared to LC50of commercial bioinsecticide Foray determined againstC. pomonella(codling moth). The activity of MPU B63 inclusions against codling moth larvae was approximately 24-fold higher than that of Foray. The results are a promising introduction for further study evaluating the potential usefulness of isolate MPU B63 crystals in plant protection.

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 5094
Author(s):  
Soledad Quiroz-Carreño ◽  
Edgar Pastene-Navarrete ◽  
Cesar Espinoza-Pinochet ◽  
Evelyn Muñoz-Núñez ◽  
Luis Devotto-Moreno ◽  
...  

The Chilean plants Discaria chacaye, Talguenea quinquenervia (Rhamnaceae), Peumus boldus (Monimiaceae), and Cryptocarya alba (Lauraceae) were evaluated against Codling moth: Cydia pomonella L. (Lepidoptera: Tortricidae) and fruit fly Drosophila melanogaster (Diptera: Drosophilidae), which is one of the most widespread and destructive primary pests of Prunus (plums, cherries, peaches, nectarines, apricots, almonds), pear, walnuts, and chestnuts, among other. Four benzylisoquinoline alkaloids (coclaurine, laurolitsine, boldine, and pukateine) were isolated from the above mentioned plant species and evaluated regarding their insecticidal activity against the codling moth and fruit fly. The results showed that these alkaloids possess acute and chronic insecticidal effects. The most relevant effect was observed at 10 µg/mL against D. melanogaster and at 50 µg/mL against C. pomonella, being the alteration of the feeding, deformations, failure in the displacement of the larvae in the feeding medium of D. melanogaster, and mortality visible effects. In addition, the docking results show that these type of alkaloids present a good interaction with octopamine and ecdysone receptor showing a possible action mechanism.


2013 ◽  
Vol 46 (1) ◽  
pp. 83-88
Author(s):  
Ramona Beşleagă ◽  
M. Tălmaciu ◽  
E. Cârdei ◽  
Nela Tălmaciu ◽  
G. Corneanu

Abstract In recent decades, positive changes have been occurred on the fruit crops protection, because of new synthetic or natural substances, more effective and less toxic. In 2011, at Fruit Growing Research and Development Station Iaşi, Romania, was tested a phitosanitary program that aimed especially to control the main pest of apple, being introduced the next generation of plant protection products, such as: Coragen, Proteus, Calypso, Decis 25 WG. The research was carried out for Idared, Golden delicious and Florina, on tow variants for each variety. Phytosanitary treatments applied for pest control were supplemented with fungicides used to combat major apple deseases. Until blooming tow treatments were applied for each variant, and after the petals fall, treatments were carried out at warning. Observation and measurements were performed after the treatments and they showed, at the end of each generation, the percentage of attacked fruits by the main apple pests. These produsts applied in a few treatments have provided a strong efficacy in combating major apple pests: codling moth (Cydia pomonella L.), fruit skin moth (Adoxophyes reticulana Hb.), leaf miner moth (Phyllonorycter sp.) and mites (Panonychus sp.). To combat lepidoptera, best result were obtained with Calypso 480 SC and Coragen insecticides, and also, products such as Decis 25 WG and Proteus, have ensured a good health in apple plantation.


2012 ◽  
Vol 45 (1) ◽  
pp. 59-64
Author(s):  
Ramona Beşleagă ◽  
E. Cârdei ◽  
M. Tălmaciu

Results on the Efectiveness of Plant Protection Products Tested at the Fruit Growing Research and Development Station Iaşi, Romania During 2010-2011, at the Fruit Growing Research and Development Station (FGRDS) Iaşi, Romania, were carried aut research on testing the effectiveness of plant protection products. The experiment was conducted in experimental polygon, in an apple orchard, the variety Idared. Pests on witch observations were made were: Cydia pomonella L., Adoxophyes reticulana, Panonychus ulmi, Phyllonorycter spp., and the products tested were: Affirm SG 095 and Voliam Targo SC 063. The products Affirm SC 095 was tested against the apple skin moth (Adoxophyes reticulana) and apple codling moth (Cydia pomonella L.), and the product Voliam Targo SC 063 was tested against red mites (Panonychus ulmi) and the leaves miners (Phyllonorycter spp.) apple codling moth (Cydia pomonella L.). As standard products Coragen and Vertimec 1.8 EC were used at the rates of 0.15 l/ha and 0.75/ha respectively.


2021 ◽  
Vol 14 (2) ◽  
pp. 99-107
Author(s):  
I. Idris ◽  
K. Hussian

Summary The codling moth [Cydia pomonella L. (Lepidoptera: Tortricidae)] is the main pest in most apple orchards in Syria. It causes billions of dollars in loss of fruit crops every year. The present work examined the effects of gamma radiation and Bacillus thuringiensis (BT) on F1 progeny of C. pomonella. The experimental design was based on two factors, namely F1 offspring produced by males irradiated at a dose of 150 Gy, and artificial diet of BT-treated larvae. The first offspring of unirradiated and irradiated C. pomonella males, F1, were from parents treated with a commercial formulation of Bacillus thuringiensis BT (Dipel® 2X). F1 progeny of unirradiated was significantly less susceptible to BT than that of irradiated parents. The results showed high mortality in F1 progeny of C. pomonella when gamma radiation and BT were applied together. The LC50 in F1 progeny was 2.5 ppm for irradiated parents, while it was 13 ppm for unirradiated ones. A significant reduction in the fecundity and egg hatchability of F1 progeny of irradiated parents compared to unirradiated ones was observed. This study demonstrated that for F1 progeny the combination between Inherited Sterility Technique and BT can be useful to achieve an integrated pest management program of codling moth.


2021 ◽  
Vol 117 (1) ◽  
pp. 1
Author(s):  
Abdelkader Nabil TIFFRENT ◽  
Nadia LOMBARKIA

<p>Codling moth (<em>Cydia pomonella</em> [L.]) is the most serious pest of apple worldwide. Its control still relies largely on insecticide applications. To deal with this situation, it becomes essential to design eco-friendly control systems to minimize chemical treatments. In this context, the effect of spraying of fructose (100 ppm), glucose (100 ppm) and insecticide (Deltamethrin), on the Golden Delicious variety against <em>C. pomonella</em> larval damages, was studied in an orchard located in Batna province (Algeria). The results of this study showed that codling moth own four generations in the study area. It is a very important pest with about 59.19 ± 1.15 % of damaged fruits at harvest. The spraying of glucose alone, fructose alone and the chemical insecticide alone causes a significant increase in the percentages of healthy fruit at harvest compared to the untreated control. The use of fructose and glucose<strong> </strong>has significantly reduced the percentage of damaged fruits at harvest followed by the spraying of the insecticide which produces the lowest percentage. The Abbott’s efficacy of glucose treatments was 23.75 ± 2.6 % compared to the insecticide 37.6 ± 2.55 %; and fructose 15.54 ± 3.01 %. The use of sugars is a completely innovative way in the field of plant protection. These first results demonstrate a promising alternative to conventional programs.</p><p><br /><strong></strong></p>


2006 ◽  
Vol 92 (2) ◽  
pp. 96-99 ◽  
Author(s):  
Rumyana Boncheva ◽  
Stefan Dukiandjiev ◽  
Ivan Minkov ◽  
Ruud A. de Maagd ◽  
Samir Naimov

2000 ◽  
Vol 48 (1) ◽  
pp. 88-92 ◽  
Author(s):  
Jesús Avilla ◽  
Antoni Teixidò ◽  
Carlos Velázquez ◽  
Nelson Alvarenga ◽  
Esteban Ferro ◽  
...  

Author(s):  
A. A. Tsygichko ◽  
A. M. Asaturova ◽  
M. D. Pavlova ◽  
N. S. Tomashevich

The insecticidal activity of strains from DBK of the Federal State Budget Scientific Institution VNIIBZR “State Collection of Entomoacarifagi and Microorganisms” was studied with respect to the test object Galleria mellonella L. and with respect to the target insect Cydia pomonella L. It was revealed that strain BZR 14 is promising for further study and development of a laboratory sample based on it.


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