A chimeric open reading frame associated with cytoplasmic male sterility in alloplasmic wheat with Triticum timopheevi mitochondria is present in several Triticum and Aegilops species, barley, and rye

Abstract Background Cytoplasmic male sterility (CMS) is a trait associated with non-functional pollen or anthers, caused by the interaction between mitochondrial and nuclear genes. Findings A Tadukan-type CMS line (TAA) and a restorer line (TAR) were obtained by successive backcrossing between the Oryza sativa cultivars Tadukan (a cytoplasmic donor) and Taichung 65 (a recurrent pollen parent). Using Illumina HiSeq, we determined whole-genome sequences of the mitochondria of TAA and screened the mitochondrial genome for the presence of open reading frame (orf) genes specific to this genome. One of these orf genes, orf312, showed differential expression patterns in TAA and TAR anthers at the meiotic and mature stages, with transcript amounts in TAR being less than those in TAA. The orf312 gene is similar to the previously described orf288, a part of which is among the components comprising WA352, a chimeric CMS-associated gene of wild-abortive-type CMS. Conclusions The orf312 gene is a promising candidate for CMS-associated gene in TAA.

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Mitochondrial RNA editing truncates a chimeric open reading frame associated with S male-sterility in maize

Current Genetics ◽

10.1007/s00294-002-0344-5 ◽

2002 ◽

Vol 42 (3) ◽

pp. 179-184 ◽

Cited By ~ 31

Author(s):

Larbi Gallagher ◽

Stephanie Betz ◽

Christine Chase

Keyword(s):

Male Sterility ◽

Rna Editing ◽

Open Reading Frame ◽

Mitochondrial Rna ◽

Reading Frame

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Sequence and expression of a fused mitochondrial gene, associated with Petunia cytoplasmic male sterility, compared with normal mitochondrial genes in fertile and sterile plants

Philosophical Transactions of the Royal Society of London Series B Biological Sciences ◽

10.1098/rstb.1988.0043 ◽

1988 ◽

Vol 319 (1193) ◽

pp. 199-208 ◽

Cited By ~ 12

Keyword(s):

Cytoplasmic Male Sterility ◽

Male Sterility ◽

Somatic Hybrids ◽

Mitochondrial Gene ◽

Transcript Abundance ◽

Coding Region ◽

S1 Nuclease ◽

Reading Frame ◽

Gene Transcripts ◽

Flanking Sequences

The mitochondrial genome of plants specifies the trait known as cytoplasmic male sterility (CMS). By comparing recombinant mitochondrial genomes present in Petunia CMS and fertile somatic hybrids, a mitochondrial DNA (mtDNA) region associated with the sterility phenotype was identified. This CMS-associated mtDNA region carries a gene fusion (termed Pcf ) which contains coding region homologous to an ATP synthase proteolipid gene ( atp 9), a cytochrome oxidase subunit II gene ( cox ll), and an unidentified reading frame, designated urf S. The 5' flanking sequences are identical to those of a normal atp 9 gene in the CMS mtDNA; the source of the 3' flanking sequences has not been identified. S1 nuclease protection experiments have identified three Pcf gene transcripts, whose termini map to the same 5' locations as three transcripts of two normal atp 9 genes in CMS and fertile plants. In a fertile Petunia line, an additional transcribed atp 9 gene with a divergent 5' flanking region was identified. Although transcript abundance of homologous atp9 genes did not vary significantly between leaves and anthers in CMS and fertile lines, Pcf gene transcripts were four to five times higher in anthers than in leaves of the CMS plants.

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Influence of nuclear background on transcription of a chimeric gene (orf256) and coxI in fertile and cytoplasmic male sterile wheats

Genome ◽

10.1139/g94-028 ◽

1994 ◽

Vol 37 (2) ◽

pp. 203-209 ◽

Cited By ~ 12

Author(s):

Jiasheng Song ◽

Charles Hedgcoth

Keyword(s):

Mitochondrial Dna ◽

Open Reading Frame ◽

Mitochondrial Rna ◽

Coding Region ◽

Male Sterile ◽

Reading Frame ◽

Cytoplasmic Male Sterile ◽

Triticum Timopheevi ◽

Nuclear Background ◽

Flanking Region

Crosses between Triticum timopheevi, as maternal donor, and T. aestivum can lead to cytoplasmic male sterile (cms) plants. The T. timopheevi derived mitochondrial DNA from parental, cms, and fertility-restored lines differs from that of T. aestivum derived mtDNA in the coxI gene region. Our previous results for cms lines showed that there is an open reading frame, orf256, upstream from coxI in T. timopheevi derived mtDNA that is not present in T. aestivum DNA. The 5′ flanking region and the first 33 nucleotides of the coding region of orf256 are identical to the corresponding region of T. aestivum coxI, whereas the rest of orf256, including the 3′ flank, is not related to coxI. Also, the organization of orf256 and coxI on a HindIII fragment from T. timopheevi derived mtDNA are identical in T. timopheevi, cms, and fertility-restored lines. We now report that the DNA sequence of orf256 is identical in T. timopheevi, cms, and fertility-restored lines. Major transcripts in cms and fertility-restored lines encode both orf256 and coxI with 5′ termini like coxI mRNA of T. aestivum, whereas parental mitochondria from T. timopheevi have major transcripts with 5′ termini within the orf256 coding region. Mitochondria from cms and fertility-restored lines have the potential to produce a protein that would not be present in parental T. timopheevi or in T. aestivum.Key words: cytoplasmic male sterility, wheat, mitochondrial DNA, mitochondrial RNA, coxI.

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