Preparation of yeast mitochondrial DNA for direct sequence analysis

2008 ◽  
Vol 54 (2) ◽  
pp. 105-109 ◽  
Author(s):  
Matus Valach ◽  
Lubomir Tomaska ◽  
Jozef Nosek
Keyword(s):  
Mitochondrial Dna ◽  
Sequence Analysis ◽  
Direct Sequence ◽  
Direct Sequence Analysis ◽  
Yeast Mitochondrial Dna

Direct Sequence Analysis of Human Herpesvirus 6 (HHV-6) Sequences from Infants and Comparison of HHV-6 Sequences from Mother/Infant Pairs

1995 ◽  
Vol 21 (4) ◽  
pp. 1017-1019 ◽  
Author(s):  
N. M. van Loon ◽  
S. Gummuluru ◽  
D. J. Sherwood ◽  
R. Marentes ◽  
C. B. Hall ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Human Herpesvirus ◽  
Direct Sequence ◽  
Human Herpesvirus 6 ◽  
Direct Sequence Analysis ◽  
Herpesvirus 6

scholarly journals Direct sequence analysis of the 14q+ and 18q- chromosome junctions in follicular lymphoma

Blood ◽  
1990 ◽  
Vol 76 (1) ◽  
pp. 131-135 ◽  
Author(s):  
F Cotter ◽  
C Price ◽  
E Zucca ◽  
BD Young
Keyword(s):  
Sequence Analysis ◽  
Direct Sequencing ◽  
Underlying Mechanism ◽  
Chromosome Translocation ◽  
Direct Sequence ◽  
Recombination Signal Sequences ◽  
Direct Sequence Analysis ◽  
Consistent Feature ◽  
Polymerase Chain ◽  
Follicular Lymphomas

Abstract Although the t(14;18) chromosome translocation has been demonstrated to be a highly consistent feature of follicular lymphomas, the underlying mechanism generating this fusion has remained uncertain. To examine this question further, a polymerase chain reaction strategy has been devised to permit the amplification and direct sequencing of the resultant 14q+ and 18q- reciprocal junctions. Direct sequence analysis of amplified 14q+ junctions established that 7 of 11 tumors contained a bcl-2 (mbr) sequence fused to an immunoglobulin JH region (five were J6 and two were J5). One of these junctions had an unusual configuration with the bcl-2 and JH sequences separated by a recognizable DH region. This finding suggests that at least some of the junctional sequences, previously thought of as N insertions, may be fragments of unrecognized DH regions. It was also possible to amplify and sequence 18q- junctions using a primer based on the DH recombination signal sequences. Several 18q- junctions were shown to consist of DH/bcl-2 (either mbr or mcr) fusions. In two tumors the 14q+ and 18q- junctions were fully sequenced, and it was demonstrated that the bcl-2 sequence was conserved during mbr and mcr translocations. This contrasts with previous analyses that demonstrated either loss or duplication of several bases at the breakpoints in the bcl-2 gene.

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