Polymerase chain reaction/direct sequence analysis of the cytochrome b gene in Salmo salar

Aquaculture ◽  
1991 ◽  
Vol 95 (3-4) ◽  
pp. 225-233 ◽  
Author(s):  
H.P. McVeigh ◽  
S.E. Bartlett ◽  
W.S. Davidson
Keyword(s):  
Polymerase Chain Reaction ◽  
Sequence Analysis ◽  
Cytochrome B ◽  
Salmo Salar ◽  
Cytochrome B Gene ◽  
Direct Sequence ◽  
Chain Reaction ◽  
Direct Sequence Analysis ◽  
Polymerase Chain

Identification of Atlantic Salmon (Salmo salar) and Rainbow Trout (Oncorhynchus mykiss) by Using Polymerase Chain Reaction Amplification and Restriction Analysis of the Mitochondrial Cytochrome b Gene

1998 ◽  
Vol 61 (4) ◽  
pp. 482-486 ◽  
Author(s):  
ESTHER CARRERA ◽  
TERESA GARCÍA ◽  
ANA CÉSPEDES ◽  
ISABEL GONZÁLEZ ◽  
BERNABÉ SANZ ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Rainbow Trout ◽  
Atlantic Salmon ◽  
Oncorhynchus Mykiss ◽  
Cytochrome B ◽  
Salmo Salar ◽  
Cytochrome B Gene ◽  
Chain Reaction ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Polymerase Chain

Restriction site analysis of polymerase chain reaction (PCR) products from a conserved region of the cytochrome b gene has been used for the identification of fresh and smoked samples of Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss). Digestion of the 359-bp PCR product with the endonucleases EcoRV and TaqI yielded specific banding patterns for salmon and trout. This genetic marker can be very useful for detecting fraudulent substitution of the cheaper smoked trout for the more expensive smoked salmon.

Polymerase Chain Reaction–Restriction Fragment Length Polymorphism Analysis of a Short Fragment of the Cytochrome b Gene for Identification of Flatfish Species

1998 ◽  
Vol 61 (12) ◽  
pp. 1684-1685 ◽  
Author(s):  
ANA CÉSPEDES ◽  
TERESA GARCÍA ◽  
ESTHER CARRERA ◽  
ISABEL GONZÁLEZ ◽  
BERNABÉ SANZ ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cytochrome B ◽  
Pcr Amplification ◽  
Cytochrome B Gene ◽  
Polymorphism Analysis ◽  
Chain Reaction ◽  
Greenland Halibut ◽  
Universal Primer ◽  
Pcr Products ◽  
Polymerase Chain

Restriction site analysis of polymerase chain reaction (PCR) products from a conserved region of the cytochrome b gene has been used for the specific identification of sole (Solea solea), European plaice (Pleuronectes platessa), flounder (Platichthys flesus), and Greenland halibut (Reinhardtius hippoglossoides). PCR amplification of the cytochrome b gene using a universal primer together with a primer specifically designed as a part of this study produced a 201-bp fragment in all species analyzed. Digestions of the PCR products with Sau3Al, BsmAl, Rsal, and Mn/l endonucleases, followed by agarose gel electrophoresis of the digested PCR products, yielded specific profiles that enabled direct identification of each species analyzed.

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