Isolation and characterization of rad51 orthologs from Coprinus cinereus and Lycopersicon esculentum, and phylogenetic analysis of eukaryotic recA homologs

To provide a better understanding of hantavirus epidemiology in China, Korean field mice (Apodemus peninsulae) and striped field mice (Apodemus agrarius) were captured in Jilin province, China, where haemorrhagic fever with renal syndrome (HFRS) is endemic. Hantavirus antigens were detected in eight of the 130 A. peninsulae individuals and in four of the 193 A. agrarius individuals by using an immunofluorescence assay. Partial S and M segments were amplified from all of the antigen-positive samples. Furthermore, two hantaviruses (CJAp89 and CJAp93) were isolated successfully in cell culture and the entire S and M segments were amplified from one of them (CJAp93). Phylogenetic analysis of these sequences (partial or complete) showed that hantaviruses carried by A. peninsulae and A. agrarius form two distinct lineages, although viruses carried by A. peninsulae are similar to those isolated previously from A. agrarius in China and from HFRS patients in Russia. However, the viruses detected in A. peninsulae in China are genetically different from those detected in A. peninsulae in other countries. These data suggest that A. peninsulae is also a natural host for HTNV in north-eastern China.

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Isolation and Characterization of Influenza C Viruses in the Philippines and Japan

Journal of Clinical Microbiology ◽

10.1128/jcm.02628-14 ◽

2014 ◽

Vol 53 (3) ◽

pp. 847-858 ◽

Cited By ~ 14

Author(s):

Takashi Odagiri ◽

Yoko Matsuzaki ◽

Michiko Okamoto ◽

Akira Suzuki ◽

Mariko Saito ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Mdck Cells ◽

Severe Pneumonia ◽

Respiratory Illness ◽

The Philippines ◽

Antigenic Analysis ◽

Isolation And Characterization ◽

Gene Sequence Analysis ◽

Influenza C Virus

From November 2009 to December 2013 in the Philippines, 15 influenza C viruses were isolated, using MDCK cells, from specimens obtained from children with severe pneumonia and influenza-like illness (ILI). This is the first report of influenza C virus isolation in the Philippines. In addition, from January 2008 to December 2013, 7 influenza C viruses were isolated from specimens that were obtained from children with acute respiratory illness (ARI) in Sendai city, Japan. Antigenic analysis with monoclonal antibodies to the hemagglutinin-esterase (HE) glycoprotein showed that 19 strains (12 from the Philippines and 7 from Japan) were similar to the influenza C virus reference strain C/Sao Paulo/378/82 (SP82). Phylogenetic analysis of the HE gene showed that the strains from the Philippines and Japan formed distinct clusters within an SP82-related lineage. The clusters that included the Philippine and Japanese strains were shown to have diverged from a common ancestor around 1993. In addition, phylogenetic analysis of the internal genes showed that all strains isolated in the Philippines and Japan had emerged through reassortment events. The composition of the internal genes of the Philippine strains was different from that of the Japanese strains, although all strains were classified into an SP82-related lineage by HE gene sequence analysis. These observations suggest that the influenza C viruses analyzed here had emerged through different reassortment events; however, the time and place at which the reassortment events occurred were not determined.

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Isolation and characterization of mutations that affect nuclear migration for dikaryosis in Coprinus cinereus

Current Genetics ◽

10.1007/s00294-003-0466-4 ◽

2004 ◽

Vol 45 (3) ◽

pp. 149-156 ◽

Cited By ~ 15

Author(s):

Rika Makino ◽

Takashi Kamada

Keyword(s):

Nuclear Migration ◽

Coprinus Cinereus ◽

Isolation And Characterization

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Isolation and characterization of a rare group A rotavirus G13P[18] strain from a diarrhoeic foal in Japan

Journal of General Virology ◽

10.1099/jgv.0.001437 ◽

2020 ◽

Vol 101 (8) ◽

pp. 800-805

Author(s):

Manabu Nemoto ◽

Hidekazu Niwa ◽

Hiroshi Kida ◽

Tohru Higuchi ◽

Yasuhiro Orita ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Group A Rotavirus ◽

Isolation And Characterization ◽

Genotype Constellation ◽

Group A ◽

First Time ◽

Rare Group

A rare genotype G13P[18] group A rotavirus (RVA/Horse-tc/JPN/MK9/2019/G13P[18]) was isolated from a diarrhoeic foal for the first time in 28 years. The genotype constellation of the virus was assigned to G13-P[18]-I6-R9-C9-M6-A6-N9-T12-E14-H11 and was the same as that of the first isolated strain, RVA/Horse-tc/GBR/L338/1991/G13P[18]. Phylogenetic analysis suggests that the virus is related to RVA/Horse-tc/GBR/L338/1991/G13P[18] and is distant from typical equine rotaviruses of the G3P[12] and G14P[12] genotypes.

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Co-Isolation and Characterization of Two Pandoraviruses and a Mimivirus from a Riverbank in Japan

Viruses ◽

10.3390/v11121123 ◽

2019 ◽

Vol 11 (12) ◽

pp. 1123 ◽

Cited By ~ 2

Author(s):

Motohiro Akashi ◽

Masaharu Takemura

Keyword(s):

Phylogenetic Analysis ◽

Dna Analysis ◽

Virus Isolation ◽

Random Amplified Polymorphic Dna ◽

Isolation And Characterization ◽

The Family ◽

Complete Sequences ◽

Infected Amoeba ◽

Giant Viruses

Giant viruses, like pandoraviruses and mimiviruses, have been discovered from diverse environments, and their broad global distribution has been established. Here, we report two new isolates of Pandoravirus spp. and one Mimivirus sp., named Pandoravirus hades, Pandoravirus persephone, and Mimivirus sp. isolate styx, co-isolated from riverbank soil in Japan. We obtained nearly complete sequences of the family B DNA polymerase gene (polB) of P. hades and P. persephone; the former carried two known intein regions, while the latter had only one. Phylogenetic analysis revealed that the two new pandoravirus isolates are closely related to Pandoravirus dulcis. Furthermore, random amplified polymorphic DNA analysis revealed that P. hades and P. persephone might harbor different genome structures. Based on phylogenetic analysis of the partial polB sequence, Mimivirus sp. isolate styx belongs to mimivirus lineage A. DNA staining suggested that the Pandoravirus spp. asynchronously replicates in amoeba cells while Mimivirus sp. replicates synchronously. We also observed that P. persephone- or Mimivirus sp. isolate styx-infected amoeba cytoplasm is extruded by the cells. To the best of our knowledge, we are the first to report the isolation of pandoraviruses in Asia. In addition, our results emphasize the importance of virus isolation from soil to reveal the ecology of giant viruses.

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