scholarly journals Isolation and Characterization of Influenza C Viruses in the Philippines and Japan

2014 ◽  
Vol 53 (3) ◽  
pp. 847-858 ◽  
Author(s):  
Takashi Odagiri ◽  
Yoko Matsuzaki ◽  
Michiko Okamoto ◽  
Akira Suzuki ◽  
Mariko Saito ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Mdck Cells ◽  
Severe Pneumonia ◽  
Respiratory Illness ◽  
The Philippines ◽  
Antigenic Analysis ◽  
Isolation And Characterization ◽  
Gene Sequence Analysis ◽  
Influenza C Virus

From November 2009 to December 2013 in the Philippines, 15 influenza C viruses were isolated, using MDCK cells, from specimens obtained from children with severe pneumonia and influenza-like illness (ILI). This is the first report of influenza C virus isolation in the Philippines. In addition, from January 2008 to December 2013, 7 influenza C viruses were isolated from specimens that were obtained from children with acute respiratory illness (ARI) in Sendai city, Japan. Antigenic analysis with monoclonal antibodies to the hemagglutinin-esterase (HE) glycoprotein showed that 19 strains (12 from the Philippines and 7 from Japan) were similar to the influenza C virus reference strain C/Sao Paulo/378/82 (SP82). Phylogenetic analysis of the HE gene showed that the strains from the Philippines and Japan formed distinct clusters within an SP82-related lineage. The clusters that included the Philippine and Japanese strains were shown to have diverged from a common ancestor around 1993. In addition, phylogenetic analysis of the internal genes showed that all strains isolated in the Philippines and Japan had emerged through reassortment events. The composition of the internal genes of the Philippine strains was different from that of the Japanese strains, although all strains were classified into an SP82-related lineage by HE gene sequence analysis. These observations suggest that the influenza C viruses analyzed here had emerged through different reassortment events; however, the time and place at which the reassortment events occurred were not determined.

scholarly journals Characterization of antigenically and genetically similar influenza C viruses isolated in Japan during the 1999–2000 season

2004 ◽  
Vol 132 (4) ◽  
pp. 709-720 ◽  
Author(s):  
Y. MATSUZAKI ◽  
S. TAKAO ◽  
S. SHIMADA ◽  
K. MIZUTA ◽  
K. SUGAWARA ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Genome Composition ◽  
Antigenic Analysis ◽  
The World ◽  
Influenza C Virus

Between October 1999 and May 2000, a total of 28 strains of influenza C virus were isolated in four Japanese prefectures: Yamagata, Miyagi, Saitama and Hiroshima. Antigenic analysis showed that the 28 isolates were divided into three distinct antigenic groups, and viruses belonging to different antigenic groups were co-circulating in each of the four prefectures. Phylogenetic analysis of the seven protein genes demonstrated that the viruses having a similar genome composition spread in various areas of Japan during the same period. Furthermore, phylogenetic analysis showed that most of the influenza C viruses isolated in various areas of the world between the 1970s and 1980s were closely related to the contemporary Japanese viruses in all gene segments. These observations suggest that the influenza C viruses cause epidemics in some communities during the same season and that antigenically and genetically similar influenza C viruses spread throughout Japan and may be circulating worldwide.

scholarly journals Isolation and Characterization of Lactic Acid Bacteria in Philippine Fermented Milkfish Chanos chanos-Rice Mixture (Burong Bangus)

2018 ◽  
Vol 6 (2) ◽  
pp. 500-508
Author(s):  
Julie Ann A. Arcales ◽  
Garner Algo L.Alolod
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Enzymatic Activity ◽  
Antimicrobial Property ◽  
Food Products ◽  
The Philippines ◽  
Lactobacillus Delbrueckii ◽  
Bacterial Strains ◽  
Isolation And Characterization

Isolation and characterization of bacteria in food products are important to determine and distinguish the beneficial or harmful effects of microbiota in certain samples. Lactic acid bacteria in food products had long been associated to good factors as food preservatives and with added fermentation metabolites. This study isolated and characterized lactic acid bacteria from burong bangus. The culture and purification process of bacteria isolation resulted to 4 strains of lactic acid bacteria namely Enterococcus faecalis, Tetragenococcus muriaticus, Lactobacillus delbrueckii subp. delbrueckii and Carnobacterium divergens. High enzymatic activity were observed with E. faecalis particularly on lipase and protease assay. While C. divergens have no enzymatic activity against lipase, protease, amylase and cellulase. The antimicrobial property of L. delbrueckii is only susceptible to amoxicillin unlike the other three bacteria isolates. No antagonistic activity were observed with the four bacterial strains against Bacillus subtilis, Staphylococcus aureus and Escherichia coli. The result of this study showed promising benefits to the industry especially in developing countries like the Philippines because population are not yet so aware of this organisms and the benefits that can be derived through their consumption.

scholarly journals Molecular characterization of porcine epidemic diarrhoea virus (PEDV) in Poland reveals the presence of swine enteric coronavirus (SeCoV) sequence in S gene

PLoS ONE ◽  
2021 ◽  
Vol 16 (10) ◽  
pp. e0258318
Author(s):  
Marta Antas ◽  
Monika Olech ◽  
Anna Szczotka-Bochniarz
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Rna Virus ◽  
Economic Losses ◽  
High Morbidity ◽  
Antigenic Analysis ◽  
S Gene ◽  
Porcine Epidemic Diarrhoea Virus ◽  
Diarrhoea Virus

Porcine epidemic diarrhoea (PED) is a highly contagious enteric viral disease of pigs with a high morbidity and mortality rate, which ultimately results in huge economic losses in the pig production sector. The etiological agent of this disease is the porcine epidemic diarrhoea virus (PEDV) which is an enveloped, positive single-stranded RNA virus. The aim of this study was to perform molecular characterization of PEDV to identify the strains circulating in Poland. In this study, 662 faecal samples from 2015 to 2021 were tested with reverse transcription quantitative real-time PCR (RT-qPCR) and the results showed that 3.8% of the tested samples revealed a positive result for PEDV. A phylogenetic analysis of the complete genome and complete S gene sequences showed that Polish PEDV strains belonged to the G1b (S-INDEL) subgroup and were closely related to the European PEDV strains isolated from 2014 to 2019. Furthermore, RDP4 analysis revealed that the Polish PEDV strains harboured a recombinant fragment of ~400 nt in the 5’ end of S gene with PEDV and swine enteric coronavirus (SeCoV) being the major and minor parents, respectively. Antigenic analysis showed that the aa sequences of neutralizing epitopes were conserved among the Polish PEDV strains. Only one strain, #0100/5P, had a unique substitution in the COE epitope. However, Polish PEDV strains showed several substitutions, especially in the COE antigen, as compared to the classical strain CV777. To the best of our knowledge, this is the first report concerning the molecular characterization of porcine epidemic diarrhoea virus strains, as well as the first phylogenetic analysis for PEDV in Poland.

Isolation and characterization of rad51 orthologs from Coprinus cinereus and Lycopersicon esculentum, and phylogenetic analysis of eukaryotic recA homologs

1997 ◽  
Vol 31 (2) ◽  
pp. 144-157 ◽  
Author(s):  
Natalie Yeager Stassen ◽  
J. M. Logsdon Jr. ◽  
G. J. Vora ◽  
Hildo H. Offenberg ◽  
Jeffrey D. Palmer ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Lycopersicon Esculentum ◽  
Coprinus Cinereus ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of hantavirus carried by Apodemus peninsulae in Jilin, China

2007 ◽  
Vol 88 (4) ◽  
pp. 1295-1301 ◽  
Author(s):  
Yong-Zhen Zhang ◽  
Yang Zou ◽  
Lai-Shun Yao ◽  
Guang-Wei Hu ◽  
Zhan-Shen Du ◽  
...  
Keyword(s):  
Cell Culture ◽  
Phylogenetic Analysis ◽  
Eastern China ◽  
Immunofluorescence Assay ◽  
Haemorrhagic Fever ◽  
Apodemus Peninsulae ◽  
Isolation And Characterization ◽  
North Eastern ◽  
Field Mice

To provide a better understanding of hantavirus epidemiology in China, Korean field mice (Apodemus peninsulae) and striped field mice (Apodemus agrarius) were captured in Jilin province, China, where haemorrhagic fever with renal syndrome (HFRS) is endemic. Hantavirus antigens were detected in eight of the 130 A. peninsulae individuals and in four of the 193 A. agrarius individuals by using an immunofluorescence assay. Partial S and M segments were amplified from all of the antigen-positive samples. Furthermore, two hantaviruses (CJAp89 and CJAp93) were isolated successfully in cell culture and the entire S and M segments were amplified from one of them (CJAp93). Phylogenetic analysis of these sequences (partial or complete) showed that hantaviruses carried by A. peninsulae and A. agrarius form two distinct lineages, although viruses carried by A. peninsulae are similar to those isolated previously from A. agrarius in China and from HFRS patients in Russia. However, the viruses detected in A. peninsulae in China are genetically different from those detected in A. peninsulae in other countries. These data suggest that A. peninsulae is also a natural host for HTNV in north-eastern China.

scholarly journals Isolation and characterization of a rare group A rotavirus G13P[18] strain from a diarrhoeic foal in Japan

2020 ◽  
Vol 101 (8) ◽  
pp. 800-805
Author(s):  
Manabu Nemoto ◽  
Hidekazu Niwa ◽  
Hiroshi Kida ◽  
Tohru Higuchi ◽  
Yasuhiro Orita ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Group A Rotavirus ◽  
Isolation And Characterization ◽  
Genotype Constellation ◽  
Group A ◽  
First Time ◽  
Rare Group

A rare genotype G13P[18] group A rotavirus (RVA/Horse-tc/JPN/MK9/2019/G13P[18]) was isolated from a diarrhoeic foal for the first time in 28 years. The genotype constellation of the virus was assigned to G13-P[18]-I6-R9-C9-M6-A6-N9-T12-E14-H11 and was the same as that of the first isolated strain, RVA/Horse-tc/GBR/L338/1991/G13P[18]. Phylogenetic analysis suggests that the virus is related to RVA/Horse-tc/GBR/L338/1991/G13P[18] and is distant from typical equine rotaviruses of the G3P[12] and G14P[12] genotypes.

scholarly journals Co-Isolation and Characterization of Two Pandoraviruses and a Mimivirus from a Riverbank in Japan

Viruses ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 1123 ◽  
Author(s):  
Motohiro Akashi ◽  
Masaharu Takemura
Keyword(s):  
Phylogenetic Analysis ◽  
Dna Analysis ◽  
Virus Isolation ◽  
Random Amplified Polymorphic Dna ◽  
Isolation And Characterization ◽  
The Family ◽  
Complete Sequences ◽  
Infected Amoeba ◽  
Giant Viruses

Giant viruses, like pandoraviruses and mimiviruses, have been discovered from diverse environments, and their broad global distribution has been established. Here, we report two new isolates of Pandoravirus spp. and one Mimivirus sp., named Pandoravirus hades, Pandoravirus persephone, and Mimivirus sp. isolate styx, co-isolated from riverbank soil in Japan. We obtained nearly complete sequences of the family B DNA polymerase gene (polB) of P. hades and P. persephone; the former carried two known intein regions, while the latter had only one. Phylogenetic analysis revealed that the two new pandoravirus isolates are closely related to Pandoravirus dulcis. Furthermore, random amplified polymorphic DNA analysis revealed that P. hades and P. persephone might harbor different genome structures. Based on phylogenetic analysis of the partial polB sequence, Mimivirus sp. isolate styx belongs to mimivirus lineage A. DNA staining suggested that the Pandoravirus spp. asynchronously replicates in amoeba cells while Mimivirus sp. replicates synchronously. We also observed that P. persephone- or Mimivirus sp. isolate styx-infected amoeba cytoplasm is extruded by the cells. To the best of our knowledge, we are the first to report the isolation of pandoraviruses in Asia. In addition, our results emphasize the importance of virus isolation from soil to reveal the ecology of giant viruses.

scholarly journals Isolation and Characterization of Diverse Halobenzoate-Degrading Denitrifying Bacteria from Soils and Sediments

2000 ◽  
Vol 66 (8) ◽  
pp. 3446-3453 ◽  
Author(s):  
Bongkeun Song ◽  
Norberto J. Palleroni ◽  
Max M. Häggblom
Keyword(s):  
Electron Acceptor ◽  
Cellular Fatty Acid ◽  
Denitrifying Bacteria ◽  
Rrna Gene ◽  
Terminal Electron Acceptor ◽  
Ecological Sites ◽  
Isolation And Characterization ◽  
Gene Sequence Analysis ◽  
Soils And Sediments

ABSTRACT Denitrifying bacteria capable of degrading halobenzoates were isolated from various geographical and ecological sites. The strains were isolated after initial enrichment on one of the monofluoro-, monochloro-, or monobromo-benzoate isomers with nitrate as an electron acceptor, yielding a total of 33 strains isolated from the different halobenzoate-utilizing enrichment cultures. Each isolate could grow on the selected halobenzoate with nitrate as the terminal electron acceptor. The isolates obtained on 2-fluorobenzoate could use 2-fluorobenzoate under both aerobic and denitrifying conditions, but did not degrade other halobenzoates. In contrast, the 4-fluorobenzoate isolates degraded 4-fluorobenzoate under denitrifying conditions only, but utilized 2-fluorobenzoate under both aerobic and denitrifying conditions. The strains isolated on either 3-chlorobenzoate or 3-bromobenzoate could use 3-chlorobenzoate, 3-bromobenzoate, and 2- and 4-fluorobenzoates under denitrifying conditions. The isolates were identified and classified on the basis of 16S rRNA gene sequence analysis and their cellular fatty acid profiles. They were placed in nine genera belonging to either the α-, β-, or γ-branch of theProteobacteria, namely, Acidovorax,Azoarcus, Bradyrhizobium,Ochrobactrum, Paracoccus,Pseudomonas, Mesorhizobium,Ensifer, and Thauera. These results indicate that the ability to utilize different halobenzoates under denitrifying conditions is ubiquitously distributed in theProteobacteria and that these bacteria are widely distributed in soils and sediments.

scholarly journals Isolation and characterization of a cDNA clone corresponding to the mouse t-complex gene Tcp-1x

1991 ◽  
Vol 57 (2) ◽  
pp. 147-152 ◽  
Author(s):  
Keith Dudley ◽  
Francis Shanahan ◽  
M. Burtenshaw ◽  
E. P. Evans ◽  
S. Ruddy ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Protein A ◽  
Northern Blotting ◽  
Chromosome 17 ◽  
T Complex ◽  
Isolation And Characterization ◽  
Gene Sequence Analysis ◽  
Acrosome Formation

SummaryThe mouse t complex on chromosome 17 is known to harbour many genes which have an important role in spermatogenesis. One of these, Tcp-1 has been cloned and shown to code for a protein probably essential for acrosome formation. During the isolation of a cDNA for Tcp-1 two other homologous sequences were recognized and described as Tcp-1x and Tcp-1y. In this paper we describe the isolation of a cDNA which has been shown by in situ hybridization to correspond to the Tcp-1x gene. Sequence analysis has confirmed that a 140 bp region of homology between Tcp-1 and Tcp-1x lies in the 3′ portion of both genes. Northern blotting has revealed that the Tcp-1x gene is expressed abundantly in liver where two transcripts are detectable and hybrid selection shows that the gene codes for a 37 kDa protein. A search of the DNA databases has failed to find any significant homology between Tcp-1x and any other sequences apart from Tcp-1.

Sign in / Sign up

Export Citation Format

Share Document