Green fluorescent protein as a visual marker for wheat transformation

2000 ◽  
Vol 19 (11) ◽  
pp. 1069-1075 ◽  
Author(s):  
M. C. Jordan
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Wheat Transformation ◽  
Green Fluorescent ◽  
Visual Marker

scholarly journals Green fluorescent protein as a visual marker in ap-nitrophenol degradingMoraxellasp.

1998 ◽  
Vol 164 (1) ◽  
pp. 187-193 ◽  
Author(s):  
Odile Tresse ◽  
Deena Errampalli ◽  
Magdalena Kostrzynska ◽  
Kam T Leung ◽  
Hung Lee ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Green Fluorescent ◽  
Visual Marker

Agrobacterium tumefaciens-mediated transformation of wheat using suspension cells as a model system and green fluorescent protein as a visual marker

2001 ◽  
Vol 28 (8) ◽  
pp. 807 ◽  
Author(s):  
Brian Weir ◽  
Xu Gu ◽  
Mingbo Wang ◽  
Narayana Upadhyaya ◽  
Adrian R. Elliott ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Agrobacterium Tumefaciens ◽  
Fluorescent Protein ◽  
Model System ◽  
35S Promoter ◽  
Suspension Cells ◽  
Agrobacterium Strain ◽  
Agrobacterium Mediated Transformation ◽  
Green Fluorescent ◽  
Visual Marker

Conditions for Agrobacterium-mediated transformation of wheat (Triticum aestivum L.) were defined using wheat suspension cells as a model system and green fluorescent protein (GFP) as a visual marker. Different strains of Agrobacterium tumefaciens were compared using established wheat cell suspension cultures, where the frequency of cell clusters showing transient activity of GFP ranged from 2 to 52%. High levels of transient GFP activity and stable transformed callus lines were obtained with plasmid pTO134 containing a gfp gene with an enhanced CaMV 35S promoter and a bar gene with a 35S promoter in combination with Agrobacterium strain AGL0. These results suggest that the important variables in Agrobacterium-mediated transformation of wheat cells include media composition, Agrobacterium strain, plasmid vector and the addition of virulence-inducing agents such as acetosyringone. The conditions deemed optimal for transformation of wheat suspension cell lines were applied to scutella isolated from immature embryos and scutella-derived calli. Transient GFP expression in these tissues ranged from 10 to 75% and, while quite variable among and within cultivars, stably transformed scutellum-derived callus was obtained. Further studies with scutellum-derived calli suggested that variables such as duration of pre-inoculation culture and co-cultivation, as well as co-cultivation temperature, were also important. Optimisation of these variables resulted in the recovery of transformed wheat plants at a transformation frequency of 1.8%, which is comparable with other reports.

The Green Fluorescent Protein (GFP)

2013 ◽  
Vol 05 (01) ◽  
pp. 101-102
Author(s):  
Phunlerd Piyaraj
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Green Fluorescent
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