Shoot regeneration in long-term callus cultures derived from mature flowering plants of Cyclamen persicum Mill.

1996 ◽  
Vol 15 (7) ◽  
pp. 545-548
Author(s):  
Willy Dillen ◽  
Ingrid Dijkstra ◽  
Johan Oud
Keyword(s):  
Shoot Regeneration ◽  
Callus Cultures ◽  
Flowering Plants ◽  
Cyclamen Persicum

Shoot regeneration in long-term callus cultures derived from mature flowering plants of Cyclamen persicum Mill.

1996 ◽  
Vol 15 (7) ◽  
pp. 545-548 ◽  
Author(s):  
Willy Dillen ◽  
Ingrid Dijkstra ◽  
Johan Oud
Keyword(s):  
Shoot Regeneration ◽  
Callus Cultures ◽  
Flowering Plants ◽  
Cyclamen Persicum

scholarly journals IMPROVED PLANT REGENERATION OF SOLANUM AND LYCOPERSICON GENOTYPES FROM LONG-TERM CALLUS CULTURE.

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1121b-1121
Author(s):  
Chang-Yeon Yu ◽  
John Masiunas
Keyword(s):  
Plant Regeneration ◽  
Shoot Regeneration ◽  
Callus Cultures ◽  
Solanum Nigrum ◽  
Regeneration Capacity ◽  
Regeneration Medium ◽  
Solanum Ptycanthum ◽  
Shoot Differentiation ◽  
Solanum Nigrum L

Repeated callus sub-culture reduce the regeneration capacity in many species. Our studies determined the effect of genotype and medium on regeneration of several Solanum and Lycopersicon genotypes from long-term callus cultures. In the first study, 13 genotypes were transferred to regeneration medium, including: Murashige and Skoog plus Gamborg Vitamins (MG); Murashige and Skoog (MS); Gamborg (GM); and white (WM). The greatest shoot regeneration was on the MG medium, containing the highest levels of thiamine. Shoot differentiation was greatest with 0.2 mg/l IAA and 2 mg/l BA. No plants were regenerated on GM or WM medium. In a second study, the effect of thiamine (0 to 200 mg/l) on shoot regeneration of the L. peruvianum genotypes PI199380, PI126945, PI251301, and PI128652, along with Solanum ptycanthum, Solanum nigrum, and L. esculentum `Diego' was evaluated. Shoot regeneration of Solanum ptycanthum, Solanum nigrum, L. peruvianum PI 199380 and PI25301 was best with 20 mg/l of thiamine.

scholarly journals Florigen governs shoot regeneration

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yaarit Kutsher ◽  
Michal Fisler ◽  
Adi Faigenboim ◽  
Moshe Reuveni
Keyword(s):  
Nitric Oxide ◽  
Shoot Regeneration ◽  
Ectopic Expression ◽  
Flowering Plants ◽  
Regeneration Capacity ◽  
No Donor ◽  
Tobacco Leaves ◽  
Age Related ◽  
Delayed Flowering

AbstractIt is widely known that during the reproductive stage (flowering), plants do not root well. Most protocols of shoot regeneration in plants utilize juvenile tissue. Adding these two realities together encouraged us to study the role of florigen in shoot regeneration. Mature tobacco tissue that expresses the endogenous tobacco florigen mRNA regenerates poorly, while juvenile tissue that does not express the florigen regenerates shoots well. Inhibition of Nitric Oxide (NO) synthesis reduced shoot regeneration as well as promoted flowering and increased tobacco florigen level. In contrast, the addition of NO (by way of NO donor) to the tissue increased regeneration, delayed flowering, reduced tobacco florigen mRNA. Ectopic expression of florigen genes in tobacco or tomato decreased regeneration capacity significantly. Overexpression pear PcFT2 gene increased regeneration capacity. During regeneration, florigen mRNA was not changed. We conclude that florigen presence in mature tobacco leaves reduces roots and shoots regeneration and is the possible reason for the age-related decrease in regeneration capacity.

Stability of Nuclear DNA Content in Long-Term Callus Cultures of Loblolly Pine

1985 ◽  
pp. 319-319
Author(s):  
Chandrasekaran I. Franklin ◽  
Ralph L. Mott ◽  
Jerome P. Miksche
Keyword(s):  
Loblolly Pine ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Callus Cultures

Long-Term Anoxia Tolerance in Flowering Plants

2011 ◽  
pp. 219-246 ◽  
Author(s):  
Robert M. M. Crawford
Keyword(s):  
Flowering Plants ◽  
Anoxia Tolerance

Cyclamen callus culture

1969 ◽  
Vol 47 (12) ◽  
pp. 2065-2067 ◽  
Author(s):  
J. R. Loewenberg
Keyword(s):  
Callus Culture ◽  
Defined Medium ◽  
Callus Cultures ◽  
Cyclamen Persicum

Cyclamen persicum callus cultures have been established on a defined medium. The tissue requires an auxin and a cytokinin. Adenine, while not required, greatly stimulates growth. The callus grows more vigorously in the dark than in light. After more than 6 years of subculture, the callus retains the capacity to form roots and shoots.

In vitro shoot regeneration and polyacetylene production in callus cultures of Lobelia erinus L.

1996 ◽  
Vol 149 (1-2) ◽  
pp. 153-156 ◽  
Author(s):  
Norie Tanaka ◽  
Koichiro Shimomura ◽  
Kanji Ishimaru
Keyword(s):  
Shoot Regeneration ◽  
Callus Cultures ◽  
In Vitro Shoot Regeneration

Plantlet regeneration from long-term callus cultures of Citrus acida Roxb. and the uniformity of regenerated plants

1999 ◽  
Vol 82 (1-2) ◽  
pp. 159-169 ◽  
Author(s):  
Bipasha Chakravarty ◽  
B.C Goswami
Keyword(s):  
Callus Cultures ◽  
Plantlet Regeneration ◽  
Regenerated Plants

In vitro shoot regeneration from mature tissue of wild Cyclamen persicum Mill.

2000 ◽  
Vol 86 (4) ◽  
pp. 323-333 ◽  
Author(s):  
Nabila S. Karam ◽  
Mohannad Al-Majathoub
Keyword(s):  
Shoot Regeneration ◽  
Cyclamen Persicum ◽  
In Vitro Shoot Regeneration
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