scholarly journals IMPROVED PLANT REGENERATION OF SOLANUM AND LYCOPERSICON GENOTYPES FROM LONG-TERM CALLUS CULTURE.

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1121b-1121
Author(s):  
Chang-Yeon Yu ◽  
John Masiunas
Keyword(s):  
Plant Regeneration ◽  
Shoot Regeneration ◽  
Callus Cultures ◽  
Solanum Nigrum ◽  
Regeneration Capacity ◽  
Regeneration Medium ◽  
Solanum Ptycanthum ◽  
Shoot Differentiation ◽  
Solanum Nigrum L

Repeated callus sub-culture reduce the regeneration capacity in many species. Our studies determined the effect of genotype and medium on regeneration of several Solanum and Lycopersicon genotypes from long-term callus cultures. In the first study, 13 genotypes were transferred to regeneration medium, including: Murashige and Skoog plus Gamborg Vitamins (MG); Murashige and Skoog (MS); Gamborg (GM); and white (WM). The greatest shoot regeneration was on the MG medium, containing the highest levels of thiamine. Shoot differentiation was greatest with 0.2 mg/l IAA and 2 mg/l BA. No plants were regenerated on GM or WM medium. In a second study, the effect of thiamine (0 to 200 mg/l) on shoot regeneration of the L. peruvianum genotypes PI199380, PI126945, PI251301, and PI128652, along with Solanum ptycanthum, Solanum nigrum, and L. esculentum `Diego' was evaluated. Shoot regeneration of Solanum ptycanthum, Solanum nigrum, L. peruvianum PI 199380 and PI25301 was best with 20 mg/l of thiamine.

scholarly journals The effect of carbon source on plant regeneration in tomato

2011 ◽  
Vol 32 (No. 1) ◽  
pp. 6-8 ◽  
Author(s):  
J. Gubiš ◽  
Z. Lajchová ◽  
L. Klčová
Keyword(s):  
Plant Regeneration ◽  
Carbon Source ◽  
Shoot Regeneration ◽  
Regeneration Capacity ◽  
Hypocotyl Explants ◽  
Cotyledon Explants ◽  
Tomato Cultivars ◽  
Number Of Shoots

 The effect of different tomato cultivars and different sugar types (sucrose, glucose and maltose) and concentrations (1.0, 2.0 and 3.0%) on shoot regeneration from aseptically grown hypocotyl and cotyledon explants were studied. Among sugar types, sucrose at a concentration of 3.0% induced the highest number of shoots from both types of explants. In hypocotyl explants, cv. Premium showed the best regeneration capacity (0.23 shoots per explant), and in cotyledon explants, cv. Hana produced the maximal number of shoots (0.43 or 0.37 for media with 2.0% or 3.0% sucrose, respectively).  

scholarly journals Effect of chemical elicitors on secondary metabolite induction of Solanum nigrum In vitro.

2014 ◽  
Vol 8 (3) ◽  
pp. 28-34
Author(s):  
Baan M. Abdulrazzak ◽  
Saadia H. Mahmood ◽  
Kadhim M. Ibrahim
Keyword(s):  
Salicylic Acid ◽  
Secondary Metabolites ◽  
Jasmonic Acid ◽  
Callus Cultures ◽  
Solanum Nigrum ◽  
Naphthalene Acetic Acid ◽  
Leaf Discs ◽  
Methanolic Extracts ◽  
Solanum Nigrum L

This project aimed to increase the production of some secondary metabolites using chemical elicitors in tissue cultures of Solanum nigrum L. plants. The quality and quantity of phytochemicals were estimated using methanolic extracts of dried leaves and callus were analyzed using HPLC. Callus was initiated from leaf discs cultured on Murashig and Skoog (MS) medium supplemented with Naphthalene acetic acid (NAA) at concentrations of 1.0, 2.0, 3.0, 4.0 or 5.0 mg/l and BA at 0.5, 1.0, 1.5, 2.0 or 2.5 mg/l for S. nigrum callus initiation. The same combination was used for callus maintenance for the plant. The results showed an increase in the concentration of secondary metabolites in methanol extracts induced from leaves. Callus cultures induced from leaf discs were treated with some chemical stimuli such as jasmonic acid and salicylic acid, (2,4,6, 8 mg/l jasmonic acid), or (50, 100, 150, 200 mg/l salicylic acid). Result showed that there were significant differences between the various treatment, the best concentration of jasmonic acid to stimulate and increase the production was 8 mg/l for S. nigrum. Moreover, Salicylic acid at 100 mg/l stimulated the production of secondary metabolites in callus culture of S. nigrum.

scholarly journals Effect of Genotype and Explant Type on Shoot Regeneration in Tomato (Lycopersicon esculentum Mill.) in vitro

2011 ◽  
Vol 39 (No. 1) ◽  
pp. 9-14 ◽  
Author(s):  
J. Gubiš ◽  
Z. Lajchová ◽  
J. Faragó ◽  
Z. Jureková
Keyword(s):  
Acetic Acid ◽  
Lycopersicon Esculentum ◽  
Shoot Regeneration ◽  
Explant Type ◽  
Regeneration Capacity ◽  
Regeneration Medium ◽  
Lycopersicon Esculentum Mill ◽  
Shoot Primordia ◽  
Indole 3 Acetic Acid

The regeneration capacity of six types of explants (segments from hypocotyl, cotyledons, epicotyl, leaf, internodes and petiole) was compared in 13 cultivars of tomato (Lycopersicon esculentum Mill). Explants were cultured on a regeneration medium containing 1 mg/l zeatin and 0.1 mg/l indole-3-acetic acid. The number of shoot primordia and shoots with 1 or more fully developed leaves was evaluated after 6 weeks. The regeneration capacity was significantly influenced by cultivars and explant types. The total number of shoot primordia produced in all types of explants was highest in the cultivars Hana and Premium and lowest in UC 82 and Money Marker. Cv. Hana also produced the highest number of shoots. The most responsive explants in most cultivars were hypocotyls and epicotyls with up to 100% regeneration and mean production of 6.3 and 6.5 shoot primordia per explant, respectively.  

scholarly journals Influence of Seedling Pretreatment and Explant Type on Watermelon Shoot Organogenesis

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 514F-515 ◽  
Author(s):  
Michael E. Compton
Keyword(s):  
Plant Regeneration ◽  
Shoot Regeneration ◽  
Shoot Organogenesis ◽  
General Procedure ◽  
Shoot Formation ◽  
Light Environment ◽  
Explant Type ◽  
Regeneration Medium ◽  
Cotyledon Explants

Several methods have been published on shoot regeneration from watermelon cotyledon explants. The major differences in regeneration protocols include the light environment in which seeds are germinated and the cotyledon region used. The purpose of these experiments was to compare the two main protocols for plant regeneration and develop one general procedure. To fulfill this objective, seeds were germinated in vitro in darkness or 16-hr light photoperiod for 7 days. Cotyledon explants from four watermelon cultivars (`Crimson Sweet', `Minilee', `Sweet Gem', and `Yellow Doll') were prepared from both dark- and light-grown seedlings. Apical and basal halves were obtained by making a cut across the cotyledon width. Apical and basal quarters were made, for comparison, by cutting apical and basal halves longitudinally. All explants were incubated on shoot regeneration medium for 6 weeks followed by a 3-week cycle on shoot elonga-tion medium. The percentage of cotyledons with shoots was 1.7-fold greater for cotyledons derived from seedings incubated in darkness than those germinated in light. Shoot formation was about 10-fold greater for explants from cotyledon basal halves and quarters than apical halves and quarters. According to these results, the best watermelon regeneration protocol should consists of basal explants from in vitro-germinated seedlings incubated in the dark for 7 days.

scholarly journals Identification of Shoot Differentiation-Related Genes in Populus euphratica Oliv.

Genes ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 1034
Author(s):  
Fu ◽  
Dong ◽  
Tan ◽  
Yin ◽  
Zhang ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
Cellular Response ◽  
De Novo ◽  
Expression Patterns ◽  
Populus Euphratica ◽  
Distinct Species ◽  
Regeneration Capacity ◽  
Shoot Differentiation ◽  
Regeneration Mechanism ◽  
Plant Pathogen Interaction

De novo shoot regeneration is one of the important manifestations of cell totipotency in organogenesis, which reflects a survival strategy organism evolved when facing natural selection. Compared with tissue regeneration, and somatic embryogenesis, de novo shoot regeneration denotes a shoot regeneration process directly from detatched or injured tissues of plant. Studies on plant shoot regeneration had identified key genes mediating shoot regeneration. However, knowledge was derived from Arabidopsis; the regeneration capacity is hugely distinct among species. To achieve a comprehensive understanding of the shoot regeneration mechanism from tree species, we select four genetic lines of Populus euphratica from a natural population to be sequenced at transcriptome level. On the basis of the large difference of differentiation capacity, between the highly differentiated (HD) and low differentiated (LD) groups, the analysis of differential expression identified 4920 differentially expressed genes (DEGs), which were revealed in five groups of expression patterns by clustering analysis. Enrichment showed crucial pathways involved in regulation of regeneration difference, including “plant hormone signal transduction”, “cell differentiation”, "cellular response to auxin stimulus", and “auxin-activated signaling pathway”. The expression of nine genes reported to be associated with shoot regeneration was validated using quantitative real-time PCR (qRT-PCR). For the specificity of regeneration mechanism with P. euphratica, large amount of DEGs involved in "plant-pathogen interaction", ubiquitin-26S proteosome mediated proteolysis pathway, stress-responsive DEGs, and senescence-associated DEGs were summarized to possibly account for the differentiation difference with distinct genotypes of P. euphratica. The result in this study helps screening of key regulators in mediating the shoot differentiation. The transcriptomic characteristic in P. euphratica further enhances our understanding of key processes affecting the regeneration capacity of de novo shoots among distinct species.

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