Experimental and computational approaches yield a high-resolution, 1-Mb physical map of the region harboring the mouse t haplotype sterility factor, tcs1

2001 ◽  
Vol 12 (8) ◽  
pp. 668-670 ◽  
Author(s):  
Antonio Planchart ◽  
John C. Schimenti
Keyword(s):  
High Resolution ◽  
Physical Map ◽  
Computational Approaches ◽  
T Haplotype

scholarly journals Atomic model of microtubule-bound tau

2018 ◽  
Author(s):  
Elizabeth H. Kellogg ◽  
Nisreen M.A. Hejab ◽  
Simon Poepsel ◽  
Kenneth H. Downing ◽  
Frank DiMaio ◽  
...  
Keyword(s):  
High Resolution ◽  
Physiological Role ◽  
Biochemical Data ◽  
Disease Pathogenesis ◽  
Developmentally Regulated ◽  
Computational Approaches ◽  
Selective Stabilization ◽  
Tubulin Binding ◽  
Future Treatments ◽  
Β Tubulin

AbstractTau is a developmentally regulated protein found in axons, whose physiological role is to stabilize and bundle microtubules (MTs). Hyper-phosphorylation of tau is thought to cause its detachment from MTs and subsequent aggregation into pathological fibrils that have been implicated in Alzheimer’s disease pathogenesis. Despite its known MT binding role, there is no consensus regarding which tau residues are crucial for tau-MT interactions, where on the MT tau binds, and how binding results in MT stabilization. We have used cryo-EM to visualize the interaction of different tau constructs with MTs at high resolution (3.2-4.8 Å) and used computational approaches to generate atomic models of tau-tubulin interactions. Our work shows that the highly conserved tubulin-binding repeats within tau adopt very similar structures in their interactions with the MT. Each tau repeat binds the MT exterior and adopts an extended structure along the crest of the protofilament (PF), interacting with both α- and β-tubulin, thus stabilizing the interface between tubulin dimers. Our structures agree with and explain previous biochemical data concerning the effect of phosphorylation on MT affinity and lead to a model in which tau repeats bind in tandem along a PF, tethering together tubulin dimers and stabilizing longitudinal polymerization interfaces. These structural findings could establish a basis of future treatments aiming at the selective stabilization of tau-MT interactions.

Construction of a high-resolution RH map of the human 2q35 region on TNG panel and comparison with a physical map of the porcine homologous region 15q25

2001 ◽  
Vol 12 (5) ◽  
pp. 380-386 ◽  
Author(s):  
Annie Robic ◽  
Jin-Tae Jeon ◽  
Virginie Rey ◽  
Valérie Amarger ◽  
Patrick Chardon ◽  
...  
Keyword(s):  
High Resolution ◽  
Physical Map ◽  
Homologous Region

A High-Resolution STS, EST, and Gene-Based Physical Map of the Hereditary Paraganglioma Region on Chromosome 11q23

Genomics ◽  
1997 ◽  
Vol 44 (2) ◽  
pp. 214-221 ◽  
Author(s):  
Bora E. Baysal ◽  
Evert M. van Schothorst ◽  
Joan E. Farr ◽  
Michael R. James ◽  
Peter Devilee ◽  
...  
Keyword(s):  
High Resolution ◽  
Physical Map ◽  
Chromosome 11Q23

scholarly journals HAPPY mapping in a plant genome: reconstruction and analysis of a high-resolution physical map of a 1.9 Mbp region ofArabidopsis thalianachromosome 4

2002 ◽  
Vol 1 (1) ◽  
pp. 23-31 ◽  
Author(s):  
Madan Thangavelu ◽  
Allan B. James ◽  
Alan Bankier ◽  
Glenn J. Bryan ◽  
Paul H. Dear ◽  
...  
Keyword(s):  
High Resolution ◽  
Physical Map ◽  
Plant Genome ◽  
Genome Reconstruction

A high-resolution physical map of equine homologs of HSA19 shows divergent evolution compared with other mammals

2005 ◽  
Vol 16 (8) ◽  
pp. 631-649 ◽  
Author(s):  
Candice Brinkmeyer-Langford ◽  
Terje Raudsepp ◽  
Eun-Joon Lee ◽  
Glenda Goh ◽  
Alejandro A. Schäffer ◽  
...  
Keyword(s):  
High Resolution ◽  
Physical Map ◽  
Divergent Evolution

scholarly journals Construction of a high-density, high-resolution genetic map and its integration with BAC-based physical map in channel catfish

DNA Research ◽  
2014 ◽  
Vol 22 (1) ◽  
pp. 39-52 ◽  
Author(s):  
Y. Li ◽  
S. Liu ◽  
Z. Qin ◽  
G. Waldbieser ◽  
R. Wang ◽  
...  
Keyword(s):  
High Resolution ◽  
Genetic Map ◽  
Channel Catfish ◽  
Physical Map ◽  
High Density

scholarly journals A 3-Mb High-Resolution BAC/PAC Contig of 12q22 Encompassing the 830-kb Consensus Minimal Deletion in Male Germ Cell Tumors

1999 ◽  
Vol 9 (7) ◽  
pp. 662-671
Author(s):  
Vundavalli V.V.S. Murty ◽  
Kate Montgomery ◽  
Shipra Dutta ◽  
Shashi Bala ◽  
Beatrice Renault ◽  
...  
Keyword(s):  
High Resolution ◽  
Germ Cell ◽  
Sequence Data ◽  
Physical Map ◽  
Molecular Genetic ◽  
Germ Cell Tumors ◽  
Suppressor Gene ◽  
Male Germ Cell ◽  
Gene Map ◽  
Link Type

Cytogenetic and molecular genetic analyses have shown that the 12q22 region is recurrently deleted in male germ cell tumors (GCTs), suggesting that this site may harbor a tumor suppressor gene (TSG). Previous loss of heterozygosity (LOH) analyses identified a consensus minimal deleted region between the markers D12S377 andD12S296, and a YAC clone contig covering the region was generated. Here, we describe a high-resolution sequence-ready physical map of this contig covering a 3-Mb region. The map comprised of 52 cosmids, 49 PACs, and 168 BACs that were anchored to the previous YAC contig; 99 polymorphic, nonpolymorphic, EST, and gene-based markers are now placed on this map in a unique order. Of these, 61 markers were isolated in the present study, including one that was polymorphic. In addition, we have narrowed the minimal deletion to ∼830 kb betweenD12S1716 (proximal) and P382A8-AG (distal) by LOH analysis of 108 normal-tumor DNAs from GCT patients using 21 polymorphic STSs. These physical and deletion maps should prove useful for identification of the candidate TSG in GCTs, provide framework to generate complete DNA sequence, and ultimately generate a gene map of this segment of the chromosome 12.[The sequence data described in this paper have been submitted to the Genome Survey Sequence under accession nos. AQ254896–AQ254955 andAQ269251–AQ269266. Online supplementary material is available athttp://www.genome.org]

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