Immunohistochemical studies on clustered pericapillary bodies in the spinal cord of post-poliomyelitis patients

1996 ◽  
Vol 92 (2) ◽  
pp. 164-169 ◽  
Author(s):  
Akito Ikemoto ◽  
A. Hirano
Keyword(s):  
Spinal Cord ◽  
Immunohistochemical Studies

Immunohistochemical studies of cholecystokininlike peptides and their relation to 5-HT, CGRP, and bombesin immunoreactivities in the brainstem and spinal cord of lampreys

1988 ◽  
Vol 271 (1) ◽  
pp. 1-18 ◽  
Author(s):  
Lennart Brodin ◽  
James T. Buchanan ◽  
Tomas Hökfelt ◽  
Sten Grillner ◽  
Jens F. Rehfeld ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Immunohistochemical Studies

scholarly journals Intraspinal Transplantation of hNT Neurons in the Lesioned Adult Rat Spinal Cord

2004 ◽  
Vol 31 (1) ◽  
pp. 87-96 ◽  
Author(s):  
Sean Dennis Christie ◽  
Damaso Sadi ◽  
Ivar Mendez
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Neuron Specific Enolase ◽  
Neural Transplantation ◽  
Adult Rat ◽  
Tissue Source ◽  
Human Neurons ◽  
Cord Injury ◽  
Female Wistar Rats ◽  
Immunohistochemical Studies

Background:The role of neural transplantation as a restorative strategy for spinal cord injury continues to be intensely investigated. Ideally, the tissue source for transplantation must be readily available, free of disease and able to survive and mature following implantation into the adverse environment created by the injury. We have studied the use of a commercially available cell line of cultured human neurons (hNT neurons) as a tissue source for neural transplantation in spinal cord injury.Methods:Following a left lateral thoracic hemisection, 54 immunosuppressed, female Wistar rats were randomly allocated into different treatment groups; hemisection only or hemisection and hNT cell transplantation (via a bridge, double or triple graft). Grafting occurred three days after spinal cord injury. After thirteen weeks the animals were sacrificed and tissue sections were stained with human neuron specific enolase and human specific neural cell adhesion molecule.Results:Immunohistochemical evidence of graft survival was displayed in 66.7% of the surviving, grafted animals. Fibre outgrowth, greatest in the bridge and triple grafts, was observed in both rostral and caudal directions essentially bridging the lesion. Double grafts were smaller, displaying less fibre outgrowth, which did not cross the lesion. Long fibre outgrowth was evident up to 2 cm from the graft as assessed by tracing and immunohistochemical studies.Conclusion:Bridge and triple grafts displayed greater growth and enabled the hNT graft to essentially bridge the lesion. This suggests that hNT neurons have the potential to structurally reconnect the proximal and distal spinal cord across the region of injury.

Expression of high molecular weight tau in the central and peripheral nervous systems

1993 ◽  
Vol 105 (3) ◽  
pp. 729-737
Author(s):  
I.S. Georgieff ◽  
R.K. Liem ◽  
D. Couchie ◽  
C. Mavilia ◽  
J. Nunez ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Molecular Weight ◽  
Nervous System ◽  
High Molecular Weight ◽  
Tumor Cells ◽  
Apparent Molecular Weight ◽  
In Vitro Transcription ◽  
The Central Nervous System ◽  
Immunohistochemical Studies

Using a novel PCR approach, we have cloned a cDNA encoding the entire high molecular weight tau molecule from rat dorsal root ganglia. The resulting 2080 bp cDNA differs from low molecular weight rat brain tau by the insertion of a novel 762 bp region (exon 4a) between exons 4 and 5. This cDNA clone is identical in sequence with a high molecular weight tau (HMW) cDNA from rat PC12 tumor cells and is closely related to a HMW tau cDNA from mouse N115 tumor cells. In vitro transcription/translation produces a protein that migrates on SDS-PAGE with the same apparent molecular weight as HMW tau purified from rat sciatic nerve. The HMW tau protein is generated from an 8 kb mRNA, which can be detected by northern blots in peripheral ganglia, but not in brain. A more sensitive assay using PCR and Southern blot analysis demonstrates the presence of exon 4a in spinal cord and in retina. In combination with immunohistochemical studies of spinal cord, these data suggest that HMW tau, though primarily in the peripheral nervous system, is also expressed in limited areas of the central nervous system, although its presence cannot be detected in the cerebral cortices.

Immunohistochemical studies of cholecystokinin-like immunoreactivity in the rat spinal cord using a monoclonal antibody

1986 ◽  
Vol 15 (2) ◽  
pp. 198 ◽  
Author(s):  
R.G. Williams ◽  
Andrea Varro ◽  
R. Dimaline ◽  
G.J. Dockray
Keyword(s):  
Spinal Cord ◽  
Monoclonal Antibody ◽  
Rat Spinal Cord ◽  
Immunohistochemical Studies

Chapter 75 Human fetal cortices and spinal cord transplanted to the anterior chamber of immunodeficient nude rats: immunohistochemical studies

1988 ◽  
pp. 583-590 ◽  
Author(s):  
Lars Olson ◽  
Ingrid Strömberg ◽  
Marc Bygdeman ◽  
Andreas Henschen ◽  
Barry Hoffer ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Anterior Chamber ◽  
Nude Rats ◽  
Immunohistochemical Studies

Immunohistochemical Studies on the Calbindin D-28K and Parvalbumin Positive Neurons in the Brain Stem and Spinal Cord after Transection of Spinal Cord of Rats

2002 ◽  
Vol 15 (4) ◽  
pp. 305 ◽  
Author(s):  
Jong Joong Kim ◽  
In Youb Chang ◽  
Yoon Young Chung ◽  
Sang Pil Yoon ◽  
Jeong Seok Moon ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Brain Stem ◽  
Calbindin D ◽  
Immunohistochemical Studies ◽  
The Brain
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