Patented natural avocado sugars modulate the HBD-2 expression in human keratinocytes through the involvement of protein kinase C and protein tyrosine kinases

2009 ◽  
Vol 302 (3) ◽  
pp. 201-209 ◽  
Author(s):  
Iole Paoletti ◽  
Elisabetta Buommino ◽  
Laura Tudisco ◽  
Caroline Baudouin ◽  
Philippe Msika ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Tyrosine Kinases ◽  
Human Keratinocytes ◽  
Protein Tyrosine Kinases ◽  
Protein Tyrosine ◽  
Kinase C

scholarly journals Mechanisms for Induction of L-Selectin Loss from T Lymphocytes by a Cryptococcal Polysaccharide, Glucuronoxylomannan

1999 ◽  
Vol 67 (1) ◽  
pp. 220-229 ◽  
Author(s):  
Zhao Ming Dong ◽  
Lydgia Jackson ◽  
Juneann W. Murphy
Keyword(s):  
T Cells ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
T Lymphocytes ◽  
Tyrosine Kinases ◽  
Jurkat Cells ◽  
Protein Tyrosine Kinases ◽  
Protein Tyrosine ◽  
Herbimycin A ◽  
Kinase C

ABSTRACT Disseminated cryptococcosis is accompanied by cryptococcal polysaccharides in the serum and the lack of cellular infiltrates in infected tissues. Cryptococcal polysaccharides given intravenously to mice inhibit the influx of T lymphocytes into the sites of cell-mediated immune response. The focus here was to determine whether cryptococcal polysaccharides modulate the expression of molecules, such as L-selectin, that are important in extravasation of T cells. Cryptococcal glucuronoxylomannan (GXM), but not galactoxylomannan or mannoprotein, was found to cause loss of L-selectin from freshly isolated human T cells of both CD4 and CD8 subsets and from Jurkat cells. With the signaling-pathway inhibitors staurosporine (which inhibits protein kinase C) and herbimycin A (which inhibits protein tyrosine kinases), we showed that GXM or the cryptococcal culture filtrate antigen CneF directly induces L-selectin loss from CD4+ and CD8+ T cells via a herbimycin A-sensitive pathway(s) presumably involving one or more protein tyrosine kinases but not via a pathway involving protein kinase C. Loss of L-selectin from the T cells before the T cells have a chance to bind to L-selectin ligands on endothelial cells would be expected to prevent T-cell migration into inflamed tissues and/or lymph organs.

Sign in / Sign up

Export Citation Format

Share Document